Micropropagation and assessment of genetic stability in Celastrus paniculatus: An endangered medicinal plant

Senapati, S. K.; Aparajita, Subhashree; Rout, Gyana Ranjan

doi:10.2478/s11756-013-0187-1

Cited by 25 publications

(13 citation statements)

References 19 publications

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“…The overall banding profile obtained from total of 2,350 bands (number of plants analysed 9 total number of scorable bands from RAPD and ISSR primers) were generated from the mother plant and 24 in vitro raised clones. All banding profiles were monomorphic in nature, similar to those of the mother plants indicating homogeneity among the regenerated culture and genetic uniformity with that of the donor plants similar to results of Senapati et al (2013), Saha et al (2014). This is the first report of genetically sustainable micropropagation protocol of O. canum from nodal explants.…”

Section: Resultsmentioning

confidence: 50%

“…A better analysis of genetic stability of plantlets can be made by using a combination of two types of markers, which amplify (Martins et al 2004). Genetic integrity by RAPD and ISSR analysis has been reported earlier in many medicinal plant species (Senapati et al 2013;Saha et al 2014). Out of a total of 30 RAPD and 17 ISSR primers, 17 RAPD and nine ISSR primers were selected based on their reproducibility (Tables 6, 7).…”

Section: Resultsmentioning

confidence: 98%

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Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims.

Saha

Roy

Sengupta

et al. 2014

Ind J Plant Physiol.

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An efficient and improved plant regeneration protocol has been developed from nodal explants of Ocimum canum Sims., a medicinally important herbaceous plant species belonging to the family Lamiaceae. Murashige and Skoog (MS) basal medium supplemented with 0.5 mg l -1 N 6 -benzyladenine (BA) showed maximum percentage of shoot multiplication (91.1 %) with 5.32 shoots per explant. Rooting of shoots occurred on 1/2 MS medium supplemented with 1.0 mg l -1 indole-3-butyric acid (IBA). Well-developed plantlets, transferred to plastic pots containing sand, soil and compost (2:2:1) showed 80.9 % survival rate under field condition. The genetic fidelity of in vitro raised field-grown plants to the donor plant was ascertained from histological, cytological and two PCR based molecular markers, i.e., random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR). Histological study showed direct organogenesis on epidermal and sub epidermal layer, where as cytological studies showed no visible anomalies within its chromosome compliments. Both RAPD and ISSR analysis revealed high degree of monomorphism, similar to those of the mother plants. This method of in vitro clonal propagation of O. canum Sims. may, therefore, could be an avenue of its sustainable commercial exploitation and conservation.

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Section: Resultsmentioning

confidence: 50%

Section: Resultsmentioning

confidence: 98%

Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims.

Saha

Roy

Sengupta

et al. 2014

Ind J Plant Physiol.

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“…Lal and Singh (2010) found the maximum number of shoots (8.9 ± 0.5) along with hundred per cent bud break in the MS medium supplemented with 1.0 mgl-1 BAP. Senapati et al (2013) observed that the Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/L 6-benzylaminopurine (BAP) and 0.1 mg/L naphthalene acetic acid (NAA) showed maximum percentage of shoot multiplication (83.4%) with 8.2 shoots/explants.…”

Section: Shoot Multiplicationmentioning

confidence: 99%

“…Lal and Singh (2010) proved that the MS half strength medium supplemented with 0.5 mgl-1 NAA best with hundred per cent rooting. Senapati et al, (2013) was achieved Maximum rooting of 73.3% with 4.8 roots/shoot on halfstrength MS media supplemented with 0.5 mg/L indole-3-acetic acid (IAA) and the percentage of survival was 91% after acclimatization.…”

Section: In Vitro Rootingmentioning

confidence: 99%

In vitro Effect of Various Plant Growth Regulator on Micro Propagation of Celastrus paniculatus: An Important Medicinal Plant

Vijay¹,

Shukla²,

Saxena³

2016

Int.J.Curr.Microbiol.App.Sci.

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“…None of the members is listed in the Convention on International Trade in Endangered Species of Wild Flora and Fauna (CITES). In vitro multiplication of threatened members like C. paniculatus has been reported by using different explants (Nair and Seeni 2001 ;De Silva and Senarath 2009 ;Senapati et al 2013 ). Information on regeneration of other threatened important members such as H. africana and C. scandens is not available yet.…”

Section: Threats and Conservationmentioning

confidence: 99%

Climber Plants: Medicinal Importance and Conservation Strategies

Ali

Isah

Dipti

et al. 2015

Biotechnological Strategies for the Conservation of Medicinal and Ornamental Climbers

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Climbing plants are groups of plants that often show unique horticultural uses because of their beauty-imparting features. As the stems are weak, these plants have evolved various climbing devices in order to support growth and development. This climbing habit is predominantly seen in angiosperms and some members of ferns, and Gnetum is the only representative genus of gymnosperm. Several families such as Convolvulaceae, Cucurbitaceae, and Dioscoreaceae are exclusively climbers, while over 50 species of families like Rubiaceae, Fabaceae, Calastraceae, and Apocynaceae are also of climber types. Besides their aesthetic use, the plants are of high medicinal value as almost all contain pharmaceutically active bio-compounds like michellamines A and B (anti-HIV properties) present in Ancistrocladus korupensis , various saponins ( Asparagus racemosus ), diosgenin ( Dioscorea deltoidea ), colchicine ( Gloriosa superba ), cordifolioside A ( Tinospora cordifolia ), momordin ( Momordica balsamina ), protoberberine, syringin, shatavarin I-IV, asparagine, aglycones, etc. Many of these plants are widely used in folk and traditional medicines. The prevalence of diseases and high cost of modern Medicare coupled with increasing load of human population across the globe have resulted in overexploitation of plants/climbers with extinction possibilities. A large number of plants including the above have already been endangered. Therefore, a balanced approach is needed in order to preserve germplasm of climbers for future uses. The application of biotechnological interventions and ex situ conservation approaches like in vitro cryopreservation and setting/strengthening germplasm or seed banks have opened a new vista for preservation of climbers. In this chapter, the history, distribution, and medicinal applications of select climbers have been discussed. Different conservation strategies have also been described in climbers, keeping various challenges in mind to its success.

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Micropropagation and assessment of genetic stability in Celastrus paniculatus: An endangered medicinal plant

Cited by 25 publications

References 19 publications

Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims.

Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims.

In vitro Effect of Various Plant Growth Regulator on Micro Propagation of Celastrus paniculatus: An Important Medicinal Plant

Climber Plants: Medicinal Importance and Conservation Strategies

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