In vitro Shoot Multiplication of Withania coagulans (Stocks) Dunal

Abstract

Withania coagulans (Stocks) Dunal is an important medicinal plant of Solanaceae. Nodal segments obtained from field grown plants were used as explants. 1 -2 cm long nodal segment with a single one bud was cultured on MS containing 2.5 mg/l thidiazuron (TDZ), 0.1 mg/l NAA and 50 mg/l adenine sulphate (AdS) resulted in formation of 5.16 shoots per node. However, vitrification was observed in all explants within one month. On the other hand nodal explants cultured on MS supplemented with 2.50 mg/l meta-topoline (mT) with 0.1 mg/l NAA and 50 mg/l AdS resulted in the formation of 4.50 healthy and uniformly grown shoots per node.

Application of machine learning in in vitro propagation of endemic Lilium akkusianum R. Gämperle

“…professor Blaauw, MS + 1.0 mg/L mT + 0.25 mg/L NAA resulted in a considerably higher shoot number per explant with 17.53 and a mean shoot length of 7.06 cm [ 27 ]. In agreement with previous studies, MS supplemented with 2.50 mg/L mT with 0.1 mg/L NAA enhanced the formation of healthy and uniformly grown shoots per node and better shoot length in Withania coagulans (Stocks) Dunal [ 45 ]. In the in vitro scale culture of Cyrtanthus contractus , a medicinal and ornamental plant, MS medium supplemented with 1–10 μM mT increased the number of shoots per explant, but fresh and dry mass of the shoots decreased in higher concentrations.…”