In vitro Shoot Proliferation and Plant Regeneration of Phlogacanthus thyrsiflorus Nees. a Rare Medicinal Shrub of Bangladesh

Abstract

An efficient protocol was developed for shoot proliferation and plant regeneration of Phlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA + 0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration D. O. I. 10.3329/ptcb.v21i2.10236 Plant Tissue Cult. & Biotech. 21(2): 135-141, 2011 (December)

Virus-Free Plant Regeneration from Shoot Apical Meristem of Coccinia grandis L., an Important Medicinal Plant in Bangladesh

“…Al Khateeb et al [27] observed the maximum number of root induction in Lavandula coronopifolia at half-strength MS with 1.0 mg l 21 IBA. In contrast, Hassan et al [30] reported the highest number of root induction in Phlogacanthus thyrsiflorus on half-strength MS with 0.5 mg l 21 IBA and 0.5 mg l 21 IAA together. Cüce and Sökmen [31] observed the number of root induction in bog bilberry on MS with IBA, IAA, and NAA individually or together.…”

“…Kaushal et al [23], Deshpande and Bhalsing [7], and Haque et al [8] also reported a similar survival rate of hardening for different plantlets that support our present findings. Hassan et al [30] also reported the highest 85% survival rate after the acclimatization of Phlogacanthus thyrsiflorus plantlets in outdoor conditions.…”

Phlogacanthus - An important medicinal plant of North East India: A review

“…(Acanthaceae), a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/L BAP + 0.5 mg/L NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture (Hassan et al, 2011).…”

“…Hassan et al (2011) developed an efficient protocol for shoot proliferation and plant regeneration of P. thyrsiflorus Nees. (Acanthaceae), a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants.…”

Analgesic, anti-inflammatory, and anti-oxidant activities of Phlogacanthus thyrsiflorus leaves

“…It is a gregarious shrub with long orange-red tubular flowers and upright spikes at the end of branches, which are the major characteristics of the plant [3]. Traditionally, PT has been used against gout and rheumatism by the tribal people of Bangladesh [4].…”