In vitro Short-Time Killing Activity of Povidone-Iodine (Isodine&lt;sup&gt;®&lt;/sup&gt; Gargle) in the Presence of Oral Organic Matter

Yoneyama, Akio; Shimizu, Masaki; Tabata, Makiko; Yashiro, Junko; Takata, Toshihiko; Hikida, Muneo

doi:10.1159/000089207

Cited by 18 publications

(21 citation statements)

References 12 publications

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“…This result was also supported by many reports which indicated that the efficacy of PVP-I declined when organic matter (e.g. blood, fish mucus, amino acids, or simple aromatic compounds) was present Yoneyama et al, 2006). Similar results also had been observed in seawater that the content of molecular iodine reduced within hours when added to seawater (Truesdale, Luther & Canosa-Masb, 1995).…”

Section: Discussionsupporting

confidence: 86%

Peer Review #3 of "Disinfection effect of povidone-iodine in aquaculture water of swamp eel (Monopterus albus) (v0.2)"

Wagner¹

2018

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The swamp eel (Monopterus albus) is an important commercial farmed fish species in China. However, it is susceptible to Aeromonas hydrophila infections, resulting in high mortality and considerable economic loss. Povidone-iodine (PVP-I) is a widely used chemical disinfectant in aquaculture, which can decrease the occurrence of diseases and improve the survival. However, environmental organic matter could affect the bactericidal effectiveness of PVP-I, and the efficacy of PVP-I in aquaculture water is still unknown. In this paper, disinfection assays were conducted to evaluate the effectiveness of PVP-I against the A. hydrophila in different types of water. We found that the effective germicidal concentration of PVP-I in outdoor aquaculture water was 25 ppm for 12 hours. In indoor aquaculture water with 10 5 CFU/mL bacteria, 10 ppm and 20 ppm of PVP-I could kill 99 % and 100 % of the bacteria, respectively. The minimal germicidal concentration of PVP-I in Luria-Bertani broth was 4000 ppm, respectively. Available iodine content assay in LB solutions confirmed that the organic substance had negative impact on the effectiveness of PVP-I, which was consistent with the different efficacy of PVP-I in different water samples. Acute toxicity tests showed that the 24h-LC 50 of PVP-I to swamp eel was 173.82 ppm, which was much lower than the germicidal concentrations in outdoor and indoor aquaculture water, indicating its safety and effectivity to control the A. hydrophila. The results indicated PVP-I can be helpful for preventing the transmission of A. hydrophila in swamp eel aquaculture.

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Section: Discussionsupporting

confidence: 86%

Peer Review #3 of "Disinfection effect of povidone-iodine in aquaculture water of swamp eel (Monopterus albus) (v0.2)"

Wagner¹

2018

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“…Data concerning the penetration of antiseptic agents into these vital tissues is scarce and the real concentrations during application under clinical conditions can only be estimated. Studies used different concentrations ranging from 0.1 % to 10 % [10-12], but no mandatory recommendations are given in literature so far. Laboratory conditions cannot be directly transferred to the situation of the bacterial biofilm in periodontal pockets in vivo .…”

Section: Discussionmentioning

confidence: 99%

Effect of Short-Time Povidone-Iodine Application on Osteoblast Proliferation and Differentiation

Schmidlin¹,

Imfeld²,

Sahrmann³

et al. 2009

TODENTJ

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Background and Objective:Povidone-iodine [polyvinylpyrrolidone-iodine complex (PVP-I)] is a broad-spectrum antimicrobial agent, frequently used in dentistry. In this study we investigated the short- and longterm effects on osteoblast number, viability, and function after short exposure to PVP-I with and without additional bone-morphogenetic protein-2 (BMP-2). Material and Methods:Confluent osteoblast-like cell line (MC3T3-E1, subclone 24) cultures were exposed to pure PVP-I solution (7.7 mg/ml) and dilutions of 1:10, 1:100 and 1:1000 for 10 seconds and washed with phosphate buffer solution. Cell proliferation and viability was determined by MTT and differentiation status by alkaline phosphatase (ALP) activity 6 days after initial plating. In a separate experiment, long-term cell proliferation, viability and function were assessed 4 weeks after PVP-I treatment by MTT and deposited calcium using an Alizarin-red staining test.Results:PVP-I decreased ALP activity substantially. Stimulation by BMP-2 recovered ALP activity to near control levels at 1:100 and 1:1000 dilutions of PVP-I. The MTT assay showed reduced proliferation of the preosteoblastic cells for all treatments, irrespective whether BMP-2 was used or not. Only at PVP-I dilutions of 1:1000 proliferation rate was back to normal levels (95.6±2.4 %). No adverse long-term effect of PVP-I on mineralization of the extracellular matrix (Alizarinred) for dilutions higher than 1:100 was observed. Interestingly, undiluted and 1:10 diluted PVP-I even showed a significant increase in mineral deposition, especially in the presence of BMP-2.Conclusion:Short-time application of PVP-I in concentrations of 1:10 and higher lead to decreased viability and impaired differentiation. However, surviving cells showed good recovery and mineralization potential.

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“…Antiseptic mouth rinse solutions are used in many clinical situations for different therapeutic and prophylactic purposes [11, 12]. Many of those are recommended for homecare practice [11].…”

Section: Introductionmentioning

confidence: 99%

Potentials of Mouthwashes in Disinfecting Cariogenic Bacteria and Biofilms Leading to Inhibition of Caries

Oyanagi¹,

Tagami²,

Matin³

2012

TODENTJ

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Objectives:The aim of this study was to compare the effects of certain commercially available mouthwashes on cariogenic bacteria and biofilms, following the acquisition of inhibition potentials of caries.Materials and Methods:Mouthwashes containing I) chlorhexidine gluconate (CHG; 0.0005% w/v), II) benzethonium chloride (BTC; 0.01% w/v), III) an essential oil (Listerine), and IV) povidone-iodine (PVP-I; 0.035% w/v) were tested on planktonic cariogenic bacteria, biofilms, and an ex vivo caries model. Bacterial aliquots were inoculated with each solution separately and vortexed for 10 seconds at room temperature. Bacterial viability was subsequently investigated by fluorescence microscopy (FM) after staining with a BacLight viability kit and the number of colony-forming units (CFUs) was counted. Similarly, mouthwash solutions were applied to artificial cariogenic biofilms, and bacterial viability of the biofilms was investigated as stated above. Inhibition potentials of two selected mouthwashes of carious lesions were investigated using biofilm-induced caries and a secondary caries model. In all steps, a phosphate-buffered saline (PBS) solution was included as a control.Results:Planktonic cariogenic bacteria and bacteria embedded in biofilms were killed in remarkably large numbers with Listerine and PVP-I treatment compared to PBS and other gargles. CFU counts also showed significant reduction after treatment with Listerine and PVP-I compared to other solutions (P<0.05). Listerine also displayed significant (P<0.05) inhibition effects in preventing the progression of demineralization.Conclusion:Bactericidal potencies of the mouthwashes varied significantly, suggesting that mouthwashes like Listerine can be useful for the prevention of caries and secondary caries.

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In vitro Short-Time Killing Activity of Povidone-Iodine (Isodine<sup>®</sup> Gargle) in the Presence of Oral Organic Matter

Cited by 18 publications

References 12 publications

Peer Review #3 of "Disinfection effect of povidone-iodine in aquaculture water of swamp eel (Monopterus albus) (v0.2)"

Peer Review #3 of "Disinfection effect of povidone-iodine in aquaculture water of swamp eel (Monopterus albus) (v0.2)"

Effect of Short-Time Povidone-Iodine Application on Osteoblast Proliferation and Differentiation

Potentials of Mouthwashes in Disinfecting Cariogenic Bacteria and Biofilms Leading to Inhibition of Caries

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