In vitro sperm maturation in sterlet, Acipenser ruthenus

Dzyuba, Borys; Cosson, Jacky; Boryshpolets, Sergii; Bondarenko, Olga; Dzyuba, Viktoriya; Prokopchuk, Galina; Gazo, Ievgeniia; Rodina, Marek; Linhart, Otomar

doi:10.1016/j.repbio.2014.01.003

Cited by 41 publications

(24 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In carp, results from Redondo et al [29] indicate that an ionic equilibration across the sperm membrane is the main factor responsible for maturation. In case of sturgeon species, it was shown that spermatozoa are not able to become activated at simple contact with external water [30]; sperm cells need a transient contact with urine, the latter getting mixed with milt prior to ejaculation [31] which is enough to render spermatozoa fully motile.…”

Section: The Maturation Stepmentioning

confidence: 99%

See 1 more Smart Citation

Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Cosson¹

2019

Biological Research in Aquatic Science

Self Cite

View full text Add to dashboard Cite

For reproduction, most fish species adopt external fertilization: their spermatozoa are delivered in the external milieu (marine-or freshwater) that represents both a drastic environment and a source of signals that control the motility function. This chapter is an updated overview of the signaling pathways going from external signals such as osmolarity and ionic concentration and their membrane reception to their transduction through the membrane and their final reception at the flagellar axoneme level. Additional factors such as energy management will be addressed as they constitute a limiting factor of the motility period of fish spermatozoa. Modern technologies used nowadays for quantitative description of fish sperm flagella in movement will be briefly described as they are more and more needed for prediction of the quality of sperm used for artificial propagation of many fish species used in aquaculture. The chapter will present some applications of these technologies and the information to which they allow access in some aquaculture species.Biological Research in Aquatic Science 2 the current knowledge with respect to (1) membrane reception of the activation signal and its transduction through the spermatozoon plasma membrane via the external membrane components such as ion channels or aquaporins; (2) cytoplasmic trafficking of the activation signal; (3) final steps of the signaling, including signal transduction to the axonemal machinery, and activation of axonemal dynein motors and regulation of their activity; (4) signaling involved in guidance processes that control the sperm/egg approach and meeting; and (5) pathways supplying energy for the short flagellar motility period of fish spermatozoa. For each step in this signaling process, quantitative methods were developed to evaluate the quality of the sperm samples that are used for aquaculture propagation of many fish species. These methods as well as examples of their usefulness for application to fish artificial reproduction are presented in the last part of this chapter. Fish sperm structure and spermatogenesisThe main traits of the flagellar mechanics of spermatozoa and the main factors that regulate their motility have been described in details in a recent review chapter by Cosson et al. [1]. Structure of fish spermatozoa: a brief presentationCompared to mammalian spermatozoa, the structure of a fish spermatozoon is qualified as "simple sperm", mostly because the flagellum structure does not include additional columns flanking the motor part (axoneme) that are present in mammal sperm. In teleost fish, spermatozoa generally have no acrosome (in contrast to chondrostean such as sturgeon), and the impenetrable chorion presents a micropyle that gives access to the membrane of the oocyte. Spermatozoa often show a spherical nucleus with homogenous, highly condensed chromatin, a nuclear fossa, a midpiece of variable size with or without a cytoplasmic channel, and one or two long flagella [2]. Moreover, fish spermatozoa can be classified into two forms, a...

show abstract

Section: The Maturation Stepmentioning

confidence: 99%

“…The oxidative stress can be evaluated by several methods. Results of these studies show that several protections against the oxidative stress are present in fish sperm cells and in the seminal fluid [31,120].…”

Section: Respiration Oxidative Stress Free Radicals and Dna Damagementioning

confidence: 99%

Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Cosson¹

2019

Biological Research in Aquatic Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…To trigger motility; sperm were diluted in AM with dilution rate 1:50 (Dzyuba, Cosson, Boryshpolets, Bondarenko, Dzyuba, Prokopchuk, Gazo, Rodina & Linhart 2014). Spermiation was stimulated by a single intramuscular injection of LHRH-A 2 (D-Ala6 GnRH Pro9-Net; Ningbo Renjian Pharmaceutical Group, Zhejiang, China) hormone at 5-10 lg kg À1 (Nazari, Modanloo, Ghomi & Ovissipor 2010) body weight 18 h before sperm collection.…”

mentioning

confidence: 99%

Retracted: Motility parameters, adenosine triphosphate content and oxidative stress indices of sterlet,Acipenser ruthenussperm after 6 days of storage

Aramli

Farsi

2015

Aquac Res

View full text Add to dashboard Cite

“…By connecting testicular and renal ducts, antioxidant 243 requirements could be met by renal secretion into urine. In agreement, in the 244 Chondrostei Acipenser ruthenus, full sperm maturation and motility was shown to 245 require dilution by urine(Dzyuba et al, 2014a). An urogenital connection is also present 246 in amphibians(Blün, 1986).…”

mentioning

confidence: 78%

A taxonomically-restricted Uric Acid transporter provides insight into antioxidant equilibrium

Oliveira

Machado

Cardoso

et al. 2018

Preprint

View full text Add to dashboard Cite

Nucleobase-Ascorbate Transporter (NAT) family includes ascorbic acid, nucleobases and uric acid transporters, with a broad evolutionary distribution. In vertebrates, four members have been previously recognized, the ascorbate transporters Slc23a1 and Slc3a2, the nucleobase transporter Slc23a4 and an orphan transporter SLC23A3. Here we identify a fifth member of the vertebrate slc23 complement (slc23a5), expressed in neopterygians (gars and teleosts) and amphibians, and clarify the evolutionary relationships between the novel gene and known slc23 genes. Further comparative analysis puts forward uric acid as the preferred substrate for Slc23a5. Gene expression quantification suggests kidney and testis as major expression sites in Xenopus tropicalis (western clawed frog) and Danio rerio (zebrafish). Additional expression in brain was detected in D. rerio, while in the Neoteleostei Oryzias latipes (medaka) slc23a5 expression is restricted to brain. The biological relevance of the retention of an extra transporter in fish and amphibians is examined: with respect to the (1) antioxidant role of uric and ascorbic acid in seminal fluid and brain, (2) the ability to endogenously synthesize ascorbic acid and (3) the morphological adaptations of the male urogenital system.

show abstract

In vitro sperm maturation in sterlet, Acipenser ruthenus

Cited by 41 publications

References 14 publications

Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Fish Sperm Physiology: Structure, Factors Regulating Motility, and Motility Evaluation

Retracted: Motility parameters, adenosine triphosphate content and oxidative stress indices of sterlet,Acipenser ruthenussperm after 6 days of storage

A taxonomically-restricted Uric Acid transporter provides insight into antioxidant equilibrium

Contact Info

Product

Resources

About