In vitro spontaneous parthenogenetic activation of golden hamster oocytes

Sun, Xingshen; Yue, Kui-Zhong; Zhou, Jiabo; Chen, Q.X.; Tan, Jing-He

doi:10.1016/s0093-691x(01)00680-x

Cited by 14 publications

(14 citation statements)

References 21 publications

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“…It is also possible that incubation in Ca 2+ ‐free conditions did not prevent the onset of spontaneous activation, but stopped its progression; once returned to Ca 2+ ‐containing medium, metaphase‐II‐stabilised rat eggs resumed meiosis. This is similar to what was previously reported for hamster eggs (Sun et al, 2002; Yoo and Smith, 2007). Limitations of excluding Ca 2+ from the culture medium include the increased tendency of incubated eggs to cytolyse and an augmented sensitivity to micromanipulation procedures (Uehara and Yanagimachi, 1977; Hayes et al, 2001).…”

Section: How Spontaneous Activation Can Be Controlledsupporting

confidence: 92%

“…It is well known that postovulatory ageing can facilitate parthenogenetic egg activation in many species, including the rat (Surani and Kaufman, 1977; Whittingham and Siracusa, 1978; Kubiak, 1989; Sun et al, 2002; Takeuchi et al, 2002; Krivokharchenko et al, 2003; Mizutani et al, 2004; Miao et al, 2009). Pre‐ovulatory rat eggs, collected from ovarian follicles, are resistant to both spontaneous and induced activation (Zernicka‐Goetz, 1991; Ben‐Yosef et al, 1995).…”

Section: What Is Spontaneous Activation and How Is It Triggered?mentioning

confidence: 99%

“…Drawing comparisons between alternative pathways of spontaneous egg activation can help to improve our understanding of this process in the rat. Spontaneous exit from metaphase‐II arrest can be observed in ovulated eggs of the golden hamster exposed to in vitro culture, but little is known about the associated biochemical events (Sun et al, 2002). Eggs obtained from c‐mos knockout mice mature normally, but frequently undergo spontaneous activation, indicating a crucial involvement of MOS‐activated pathway in faithful completion of oocyte meiotic maturation (Colledge et al, 1994; Hashimoto et al, 1994; Choi et al, 1996).…”

Section: Mechanisms Of Spontaneous Egg Activationmentioning

confidence: 99%

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Rat eggs cannot wait: Spontaneous exit from meiotic metaphase‐II arrest

Chebotareva

Taylor

Mullins

et al. 2011

Molecular Reproduction Devel

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SUMMARYMammalian eggs await fertilisation while arrested at the second metaphase stage of meiotic division. A network of signalling pathways enables the establishment and maintenance of this metaphase-II arrest. In the absence of fertilisation, mammalian eggs can spontaneously exit metaphase II when parthenogenetically stimulated, or sometimes without any obvious stimulation. Ovulated rat eggs abortively release from metaphase-II arrest once removed from egg donors. Spontaneously activated rat eggs extrude the second polar body and proceed to the so-called metaphase III-'like' stage, with clumps of condensed chromatin scattered in the egg cytoplasm. It is still unclear what makes rat eggs susceptible to spontaneous activation; however, a vague picture of the signalling pathways involved in the process of spontaneous activation is beginning to emerge. Such cell cycle instability is one of the major reasons why it is more difficult to establish nuclear transfer in the rat. This review examines the known predisposing factors and biochemical mechanisms involved in spontaneous activation. The strategies used to prevent spontaneous metaphase-II release in rat eggs will also be discussed.Mol.

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Section: How Spontaneous Activation Can Be Controlledsupporting

confidence: 92%

Section: What Is Spontaneous Activation and How Is It Triggered?mentioning

confidence: 99%

Section: Mechanisms Of Spontaneous Egg Activationmentioning

confidence: 99%

See 1 more Smart Citation

Rat eggs cannot wait: Spontaneous exit from meiotic metaphase‐II arrest

Chebotareva

Taylor

Mullins

et al. 2011

Molecular Reproduction Devel

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“…Hamster oocytes can be activated spontaneously when cultured in vitro [9,10]. Gwatkin [11] found that electrical stimulation was highly effective in activation of hamster oocytes.…”

Section: Introductionmentioning

confidence: 99%

Effects of Electrical Pulse and 6-DMAP on Cleavage of Golden Hamster Oocytes—Morphological and Phisiological Observations

Wang¹,

Jiang²,

Li³

2016

JFMK

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Abstract:The golden hamster is a well-established model system for studies of morphology, reproductive physiology, oncology, genetics and virology. The aim of this study was to establish experimental protocols necessary for cloning the golden hamster; we examined and optimized conditions for parthenogenesis and cleavage of its oocytes. We tested oocytes of different ages, including 15 h after Human chorionic gonadotropin (hCG), with two treatments: (1) an electrical pulse ranging from 10 to 600 V/mm and (2) incubation for 2 to 6 h in 2 mM 6-dimethylaminopurine (6-DMAP). These two conditions were tested both separately and in combination. We found that (i) in oocytes of different ages, cleavage exhibits a strength-dependent increase; (ii) 6-DMAP stimulates oocyte cleavage, but the cleavage rates are significantly low; and (iii) a combined treatment is more effective than a treatment with 6-DMAP alone, and is comparable to those achieved with high pulse stimuli. These results elucidate certain parameters important for the cloning of the golden hamster species.

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“…Extracellular calcium is required for spontaneous activation. Ooyctes cultured in calcium free medium or medium containing the calcium antagonist decreases spontaneous activation (10–11). One mouse strain, LT/Sv, displays a high incidence of spontaneous activation during in vivo and in vitro maturation (14–16).…”

mentioning

confidence: 99%

Strain-specific spontaneous activation during mouse oocyte maturation

Cheng

Zhong

Latham

2012

Fertility and Sterility

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Objective To examine whether spontaneous oocyte activation is determined by genetic differences and interacted with culture environment. Design Experimental Study. Setting Temple University School of Medicine. Animals C57BL/6, DBA/2, C3H/HeJ, and A/J strains, along with reciprocal F1 hybrid female mice (5–6 weeks). Intervention(s) Immature oocytes from different mouse strains were collected and cultured in different maturation conditions including different serum, serum replacement, bovine serum albumin (BSA) and follicle stimulation hormone (FSH). Main Outcome Measure(s) The emission of first polar body, pronucleus formation, meiotic arrest, spontaneous activation, and expression of maturation regulators. Result(s) Oocytes from C57BL/6 mice display a high rate of delayed first meiotic division and spontaneous activation after the first meiotic division with in vitro maturation (IVM), and the second meiosis with in vivo maturation (VVM) following superovulation. Spontaneous activation with IVM is sensitive to culture environment. Oocytes spontaneously activated during the first meiotic division with IVM have unusual paired tetrad chromosomes with slight connections at centromeres, whereas oocytes activated in vivo display haploidization from the second meiosis. Spontaneous activation is also seen in F1 hybrid oocytes, indicating a dominant trait from C57BL/6. Delayed meiosis was associated with reduced cylcin B and securin expression. Conclusion(s) Both mouse strain and culture environment have a significant effect on the incidence of meiotic defects and spontaneous activation. Reduced expression of meiotic regulators may underlie this effect.

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In vitro spontaneous parthenogenetic activation of golden hamster oocytes

Cited by 14 publications

References 21 publications

Rat eggs cannot wait: Spontaneous exit from meiotic metaphase‐II arrest

Rat eggs cannot wait: Spontaneous exit from meiotic metaphase‐II arrest

Effects of Electrical Pulse and 6-DMAP on Cleavage of Golden Hamster Oocytes—Morphological and Phisiological Observations

Strain-specific spontaneous activation during mouse oocyte maturation

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