In Vitro Studies in Sauropus Androgynous (L.) Merr.

Establishment of Plantlet Regeneration System from Nodal, Internodal and Leaf Explants of Sauropus androgynus (Sweet Shoot)

“…Earlier studies reported poor callusing from both nodal and leaf explants of sweet shoot. This might be due to the slow growth and the tendency towards the incidence of necrosis in sweet shoot calluses (Li and He, 2006;Tejavathi et al, 2010;Eganathan and Parida, 2012;Long et al, 2010). Based on the above mentioned results, leaf explants had a higher callus production capacity than internodal and nodal explants and the differences were significant.…”

“…The most commonly adopted procedure involved surface sterilization of nodal explants of sweet shoot with 0.1% (w/v) mercuric chloride for 10 min followed by three rinses with sterile purified water (Li and He, 2006;Eganathan and Parida, 2012). Shortly after Li and He's research (Sai and Srividya, 2002;Tejavathi et al, 2010) also treated nodal, shoot tip and leaf explants using Tween 20 for 15 min followed by 0.1% (w/v) Bavistin for 10 min (Tejavathi et al, 2010). However, the use of mercuric chloride and fungicides in explant surface sterilization is not recommended due to potential toxicity to both the researcher and the environment and furthermore, there is also an increased risk of plant growth retardation (Matthew and Duncan, 1993).…”

“…To date, scientific studies assessing the potentially important benefit of sweet shoot for use as medicinal plants are still limited with only four scientific studies reporting on the micropropagation of sweet shoot (Li and He, 2006;Tejavathi et al, 2010;Eganathan and Parida, 2012;Philomena, 1993). Indirect shoot development is the common regenerative pathway observed in nodal, internodal and leaf explants of sweet shoot, where the explants are cultured on MS medium supplemented with 2.0 mg LG 1 BAP and 0.5 mg LG 1 IAA to promote adventitious shoot proliferation.…”

“…Several attempts have been made to enhance shoot formation using different types and combinations of auxins and cytokinins (Li and He, 2006;Tejavathi et al, 2010;Eganathan and Parida, 2012;Philomena, 1993). In this study, the combination of BAP and IAA was proven to be effective in producing sweet shoot plantlets from leaf, nodal and internodal explants, which supports the findings from Li and He (2006).…”

“…In Philomena (1993) studied the effects of coconut water on induction and growth of shoots from nodal explants of sweet shoot and the plantlets showed increased shoot formation and growth with NAA, whereas kinetin had no effect (Philomena, 1993). The addition of adenine sulphate (10.86 μM) and gibberellic acid (1.44-2.88 μM) into L2 medium also facilitated direct regeneration of sweet shoots with roots, however, the published data was not analyzed in detail (Tejavathi et al, 2010). Furthermore, Eganathan and Parida (2012) also reported that maximum number of shoots (10.13 shoots) and shoot length (2.07 cm) was observed on MS medium containing 1.0 mg LG 1 BAP and 0.1 mg LG 1 kinetin (Eganathan and Parida, 2012).…”