In vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium

Lee, E N; Sunwoo, Hoon H.; Menninen, K; Sim, J. S.

doi:10.1093/ps/81.5.632

Cited by 121 publications

(120 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nutrient utilization was calculated by subtracting amounts of nutrient in excreta from the amount of nutrients in consumed feed. To determine the concentration of IgY, the water-soluble fraction (WSF) of egg yolk was separated according to the methods described by Bizhanov et al (2004), and the WSF was used to quantify IgY concentration by the protocol of ELISA kit (E30-104) as described by Lee et al (2002) using ELISA reader (Power Wave XS, BioTek, USA). The concentration of IgY and standard antibody were measured at absorbance of 450 nm, and the test repeated three times and the mean values of optical density were obtained.…”

Section: Measured Items and Analysesmentioning

confidence: 99%

Effect of Dietary β-Mannanase Supplementation and Palm Kernel Meal Inclusion on Laying Performance and Egg Quality in 73 Weeks Old Hens

Lee¹,

Kim²,

Lee³

et al. 2013

Journal of Animal Science and Technology

View full text Add to dashboard Cite

This study was conducted to evaluate the effect of dietary β-mannanase supplementation and palm kernel meal (PKM) inclusion (5%) on laying performance, egg quality and nutrient utilizability of laying hens with 73 weeks of age. A total of 240 Lohmann brown laying hens with average 77.5% egg production were randomly allocated with 60 hens per treatment, 4 replicates per treatment and 15 hens per replicate. Experimental design was a completely randomized design with 2 × 2 factorial arrangement, with the factors being (1) two levels of PKM (0 vs. 5%) and (2) with or without dietary β-mannanase (480 IU/kg of diet CTCzyme ® ) supplementation. All hens were housed in cages (35 cmW × 35 cmD × 40 cmH) with 2 hens per cage for six weeks feeding trial. Laying performance was recorded daily during feeding trial. Egg quality, nutrients utilizability and blood assays were done at the end of feeding trial. Egg production was improved (P<0.05) by both dietary PKM inclusion and β-mannanase combined supplementation. Either β-mannanase or PKM did not affect feed intakes and feed conversion ratio of all diets. Egg weight of hens fed diet containing 5% of PKM had heavier (P<0.05) eggs compared with hens fed without PKM. Albumen height was improved (P<0.05) by dietary mannanase supplementation. Crude fat utilization of 5% PKM diet was higher than that of no PKM diet regardless of β-mannanase supplementation. Both DM and total carbohydrate utilization were decreased (P<0.05) in hens fed 5% PKM diet. Serum IgG and yolk IgY contents of PKM groups were lower (P<0.05) than those of no PKM groups. This result showed that 5% PKM diet, independent of dietary β-mannanase supplementation, was able to improve egg production. In addition, dietary β-mannanase supplementation could be used for improving the albumen height of eggs.

show abstract

Section: Measured Items and Analysesmentioning

confidence: 99%

Effect of Dietary β-Mannanase Supplementation and Palm Kernel Meal Inclusion on Laying Performance and Egg Quality in 73 Weeks Old Hens

Lee¹,

Kim²,

Lee³

et al. 2013

Journal of Animal Science and Technology

View full text Add to dashboard Cite

show abstract

“…The concentrations of specific IgY antibodies that reacted with EPEC, STEC O111, and STEC O157 in IgY preparations were determined by ELISA as previously described by Lee et al (2002) with modifications. A bacterial culture in BHI was heated at 56°C for 30 minutes, centrifuged, and resuspended in carbonate-bicarbonate buffer, pH 9.6, to an O.D.…”

Section: Determination Of Specific Igymentioning

confidence: 99%

In vitro reactivity and growth inhibition of EPEC serotype O111 and STEC serotypes O111 and O157 by homologous and heterologous chicken egg yolk antibody

et al. 2007

View full text Add to dashboard Cite

IgY is a chicken egg yolk antibody which has been used for treatment and prophylaxis of gastrointestinal infections. Our aim was to verify if IgY obtained from chickens immunized with EPEC O111, STEC O111 and STEC O157 is able to show in vitro reactivity and biological activity towards the three bacteria. IgY was obtained from eggs laid before and after immunization with each bacterium. The preparations of IgY anti-EPEC O111 and anti-STEC O111 shared high reactivity detected by ELISA and growth inhibition ability towards both bacteria EPEC O111 and STEC O111. Nevertheless, the preparation of IgY anti-STEC O157 showed high reactivity and growth inhibitory effect only towards the homologous strain. Our results showing in vitro biological activity of IgY reinforce its use as an alternative for the treatment or prophylaxis of E. coli infections and encourage the development of in vivo studies for a possible future human therapeutic use.

show abstract

“…Cross-reactions were frequently observed between S. Enteritidis and S. Typhimurium infections when LPS fractions were used as diagnostic antigens 10,11 . Although the usual cross-reactions have been encountered, ELISA based on the LPS of S. Enteritidis could be used to screen Salmonella infections due to microorganisms of serogroups B and D. However, LPSbased ELISA is not capable to discriminate antibody response developed towards non-zoonotic and nonflagellated S. Pullorum and S. Gallinarum from that developed against flagellated zoonotic microorganisms such as S. Enteritidis and S. Typhimurium.…”

Section: Discussionmentioning

confidence: 99%

Tavuklarda Salmonella Enfeksiyonlarının Serolojik Tanısı ve Doğrulanmasına Yönelik LPS ve Flagelline Dayalı Modeller

Fındık¹,

Büyüktanir²,

Yurdusev³

2009

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

SummaryAvian salmonellosis due to Salmonella B and D serogroups has a great importance because of the public health and the economical losses in poultry industry. For accurate diagnosis of avian salmonellosis, a total of 126 chicken sera evaluated with a commercial Salmonella Enteritidis (SE) ELISA kit have been used to develop two indirect ELISA models using lipopolysaccharide (LPS) and purified flagellin (Flg) of SE as diagnostic antigens. After optimization, 154 and 263 sera were tested with indirect LPS-and Flg-ELISA, respectively. The performance of both indirect LPS-and Flg-ELISA was found high and similar for detecting anti-Salmonella antibody in comparison with a commercial LPS-ELISA kit. Nevertheless, a limited number of chicken sera were found discordant between Flg-and LPS-ELISAs. Western-blot analyses of the discordant sera demonstrated that they were found reactive with bacterial lysate of SE but only recognized LPS or Flg antigen. It was concluded from this study that the simultaneous use of both LPS-and Flg-ELISAs would allow differential diagnosis of avian specific salmonellosis from zoonotic Salmonella infections due to flagellated bacteria and Western blot models can be used as confirmatory serological test. .

show abstract

In vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium

Cited by 121 publications

References 30 publications

Effect of Dietary β-Mannanase Supplementation and Palm Kernel Meal Inclusion on Laying Performance and Egg Quality in 73 Weeks Old Hens

Effect of Dietary β-Mannanase Supplementation and Palm Kernel Meal Inclusion on Laying Performance and Egg Quality in 73 Weeks Old Hens

In vitro reactivity and growth inhibition of EPEC serotype O111 and STEC serotypes O111 and O157 by homologous and heterologous chicken egg yolk antibody

Tavuklarda Salmonella Enfeksiyonlarının Serolojik Tanısı ve Doğrulanmasına Yönelik LPS ve Flagelline Dayalı Modeller

Contact Info

Product

Resources

About