In Vitro Studies on Methanogenic Rumen Bacteria

Oppermann, R. A.; Nelson, W.O.; Brown, Richard E.

doi:10.3168/jds.s0022-0302(57)94554-x

Cited by 44 publications

(15 citation statements)

References 12 publications

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“…1. The numerous unsuccessful attempts to obtain the utilization of propionic acid by rumen enrichment cultures (Oppermann et al 1957;Nelson et al 1958) are consistent with the observations of McNeill & Brown (1954), and strongly suggest that propionate is not a substrate for rumen methanogenesis.…”

Section: Introductionsupporting

confidence: 76%

“…after the animal had been fed the standard roughage +concentrate ration, except in one trial as will be noted. This period in the rumen digestion cycle was postulated to be the most favourable for acetate utilization because : (a) the approximate 1 : 1 ratio of carbon dioxide to methane found a t this period (Nelson, Brown & Kingwill, 1960) approaches the actual (Oppermann et al 1957) and theoretical (Buswell, 1936) composition of gas obtained from the methanogenic dissimilation of acetate; (b) the acetate concentration in rumen fluid is relatively constant at this period (Brown, 1954); (c) formate and carbon dioxide, which may be preferentially utilized and/or be inhibitory to acetate utilization (Oppermann et al 1957), would be at negligible ( formate) and minimum (carbon dioxide) concentrations.…”

Section: Methodsmentioning

confidence: 99%

“…However, the existence of acetate-utilizing methane bacteria in the rumen (Beijer, 1952 ;Oppermann et al 1957; Nelson et al 1958; Yahiro, 1959) suggests that some methane may arise from this source as it does in the anaerobic fermentation of certain industrial wastes ( Buswell, 1936). The present investigation was conducted to measure the extent of the in vivo conversion of acetate to methane in the bovine rumen.…”

Section: R a Oppermann W 0 Nelson And R E Brownmentioning

confidence: 99%

“…Beijer (1952) detected a Methunosarcinu sp. in the goat rumen, and Oppermann, Nelson & Brown (1957) demonstrated Methanobacterium formicicum and a methanogenic acetate-utilizer biochemically resembling Methunobacterium sohngenii in enrichment cultures from cattle rumen contents. Nelson, Oppermann & Brown (1958) reported that rumen enrichment cultures stabilized to valeric or butyric acid contained methane bacteria which biochemically resembled Methanobacterium suboxydans.…”

Section: Introductionmentioning

confidence: 99%

“…Volatile fatty acids (VFA) present in the rumen were determined by a modification of the chromatographic method of Neish (1949) as described in a previous report by Oppermann et al (1957). The rumen fluid samples were preserved for analysis by acidification with 10 N-sulphuric acid and were immediately frozen for storage until analysis could be made.…”

mentioning

confidence: 99%

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In vivo Studies of Methanogenesis in the Bovine Rumen: Dissimilation of Acetate

Oppermann

Nelson

Brown

1961

Journal of General Microbiology

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SUMMARYThe introduction of sodium acetate-2-14C into a bovine rumen resulted in the in vivo labelling of the rumen gases and volatile fatty acids. The relative isotope concentration in substrate and products indicated that a maximum of 5.6 % of the methane and 11% of the carbon dioxide in rumen gas might have been derived from the methyl carbon of acetate when the substrate was added to the rumen 18 hr. after the animal had been fed a normal ration, A maximum of 3-53 % of the methane and 4.2 % of the carbon dioxide might have been derived from the methyl carbon of sodium acetate-2-14C when this substrate was introduced into the rumen immediately after the animal had been fed. The addition of sodium acetate-l-14C to the rumen 18 hr. after feeding indicated that 2 yo of the methane and 10 yo of the carbon dioxide was derived from the carboxyl carbon of the substrate. Most of the derived radioactivity of the volatile acids of the rumen was found in the butyric acid fraction, although smaller amounts appeared in propionic acid and the volatile fatty acids with a chain length of greater than 4 carbons.

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Section: Introductionsupporting

confidence: 76%