In vitro study of monocyte viability during the initial adhesion to albumin- and fibrinogen-coated surfaces

Characterization of microglial attachment and cytokine release on biomaterials of differing surface chemistry

“…The data are consistent with previous results showing reduced monocyte attachment on polyethylene oxide (PEO) or polyethylene glycol (PEG) [77,78] as well as on albumin [79]. While an explicit reduction in monocyte attachment has not been demonstrated on alginate, previous studies have observed a reduced foreign body reaction adjacent to alginate-coated implants [80,81].…”

Adsorption Force of Fibronectin on Various Surface Chemistries and Its Vital Role in Osteoblast Adhesion

“…Selection of 2 hours seeding is because that the time-point of 2 hours after cell seeding is generally regarded as the stage of initial cell adhesion. 50 Consistent with our deduction, the chemistry-dependence of initial FAs and FBs formation (-OH > -NH 2 ≈ -CH 3 , Figure 5) is the same as that of the accessibility of cell binding domains (-OH > -NH 2 ≈ -CH 3 , Figure 3). …”

The modulation of dendritic cell integrin binding and activation by RGD-peptide density gradient substrates

“…These data demonstrate that albumin-blocked gradient chips provide a uniform adherent cell density across the chip that is not affected by the density of RGD peptide. This finding is expected, as it is well known that leukocytes, including DCs, are capable of adhering to albumin-blocked substrates [8,34,62]. This uniform adhesion across the length of the peptide gradient is advantageous, as it removes the confounding factor of a variable adherent cell density along the gradient as is typically seen in adhesive gradient experiments with non-leukocytes.…”