Adsorption Force of Fibronectin on Various Surface Chemistries and Its Vital Role in Osteoblast Adhesion

Abstract

The amount, type, and conformation of proteins adsorbed on an implanted biomaterial are believed to influence cell adhesion. Nevertheless, only a few research works have been dedicated to the contribution of protein adsorption force. To verify our hypothesis that the adsorption force of protein on biomaterial is another crucial mediator to cell adhesion, fibronectin (FN) adsorbed on self-assembled monolayers (SAMs) with terminal -OH, -CH3, and -NH2 was quantified for FN adsorption force (F(ad)) by utilizing a sphere/plane adsorption model and parallel plate flow chamber. As revealed, F(ad) on SAMs followed a chemistry-dependence of -NH2 > -CH3 ≫ -OH. It is further demonstrated that F(ad) together with FN conformation could regulate the late osteoblast adhesion and the consequent reorganization of the adsorbed FN and fibrillogenesis of the endogenous FN. Our study suggests that protein adsorption force plays a key role in cell adhesion and should be involved for better biomaterial design.

Preferential adsorption of cell adhesive proteins from complex media on self-assembled monolayers and its effect on subsequent cell adhesion

“…7a), suggesting surface-dependent conformational change of adsorbed Fn. Conformational change of adsorbed Fn has been widely studied using SAMs [19,20,22,44,45] and polymer substrates [11,14,16]. Keselowsky et al reported that surface functional groups of SAMs modulate conformation of adsorbed Fn qualitatively determined by binding of monoclonal antibody against their cell binding: OH-> COOH-= NH 2 -> CH 3 -SAMs [19].…”

“…SAMs carrying four different functionalities (CH 3 -, OH-, COOH-, NH 2 -) were chosen since they are typical functional groups present in polymer substrates and hence commonly examined for protein adsorption and cell adhesion studies [3][4][5][6][7][8][9][10][17][18][19][20][21][22]. Surface properties of the SAMs were characterized by XPS and water contact angle measurements ( Table 1).…”

“…Role of Fn and Vn in cell adhesion on material surfaces has been extensively studied using polymer substrates [11][12][13][14][15][16] and SAMs [17][18][19][20][21][22]. Pretreatment of artificial materials with a solution containing either Fn only or Vn only improves http://dx.doi.org/10.1016/j.actbio.2015.08.033 1742-7061/Ó 2015 Acta Materialia Inc.…”

The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior

“…When an acidic degradable polymeric biomaterial is implanted for bone regeneration in the body, the host cell biological responses to the bone graft may be mainly dependent on the cell/material interaction, which can be regulated by material characteristics such as surface topography 34 , surface hydrophilicity 35, 36 , and chemical composition 37 . Luo et al .…”

Growth and Functionality of Cells Cultured on Conducting and Semi-Conducting Surfaces Modified with Self-Assembled Monolayers (SAMs)

“…The design of surfaces to elicit specific cellular responses has, accordingly, been delayed. In this regard, SAMs have the capability to produce well-defined surfaces with known structures and properties that may be carefully regulated and manipulated [6,[13][14][15][16]. Cell attachment may also be influenced by the increased absorption of fibronectin on SAMs [17] or binding of the protein in an orientation such that cell binding sites are accessible [18].…”