In vitro study of mutagenic potential of Bidens pilosa Linné and Mikania glomerata Sprengel using the comet and micronucleus assays

Mikania glomerata and M. laevigata: Clinical and Toxicological Advances

“…DNA damage was observed in rat hepatoma cells treated with hydroalcoholic maceration (10 and 20 μL/mL) and infusion (20 and 40 μL/mL) of the leaves. The genotoxic potential of the infusion was also observed by the micronucleous test at a very high concentration (40 μL/mL), suggesting a limitation in the phytotherapeutic use of guaco species (Costa et al, 2008). Caution is recommended for patients who use lyophilized extracts or medicines containing isolated compounds, such as coumarin and o-coumaric acid.…”

Mikania glomerata Spreng. e M. laevigata Sch. Bip. ex Baker, Asteraceae: estudos agronômicos, genéticos, morfoanatômicos, químicos, farmacológicos, toxicológicos e uso nos programas de fitoterapia do Brasil

“…Os estudos também relataram que existe uma diferença significativa na concentração e na composição química no extrato e na infusão, onde se verificou um teor de cumarina 55 vezes maior no extrato que na infusão. No entanto, não foi evidenciada nenhuma relação direta desse marcador com a proteção ou os danos causados ao DNA em hepatócitos (Costa et al, 2008). Com relação a estudos clínicos toxicológicos de associações de plantas contendo Mikania glomerata, existem apenas dois relatos na literatura para a avaliação da segurança clínica (Soares et al, 2006;Tavares et al, 2006).…”

“…Bras. IV, 2005. Tricoma glandular peltado e tricoma glandular unisseriado, com variável número de células na base e com célula terminal esférica ou espatulada são encontrados em depressão na epiderme, sendo este em maior frequência Lucas, 1942;Oliveira et al, 1984Oliveira et al, , 1985aOliveira et al, , 1993Oliveira et al, , 1994Vilegas et al, 1997;Veneziani et al, 1999;Fierro et al, 1999;Magalhães, 2000;Franchi, 2000;Cabral et al, 2001;Moura et al, 2002;Lima & Biasi, 2002;Castro, 2002;Yatsuda et al, 2005Costa et al, 2008;Silva et al, 2008. Cumarina; diidrocumarina; ácido o-cumárico Oliveira et al, 1984Oliveira et al, , 1994Biguetti, 1999;Magalhães, 2000;Lima & Biasi, 2002;Biavatti et al, 2004;Bighetti et al, 2005;Yatsuda et al, 2005;Santos et al, 2006;Graça et al, 2007b. Oliveira et al, 1984Oliveira et al, , 1985aOliveira et al, , 1993Oliveira et al, , 1994Oliveira et al, , 1999Vilegas et al, 1997;Veneziani et al, 1999;Fierro et al, 1999;Moura et al, 2002;Amaral et al, 2003;Yatsuda et al, 2005.…”

Antidiabetic and genotoxic effects on Wistar rats treated with aqueous extract from Chrysobalanus icaco L.

“…2334-0061. they have greater efficacy and fewer collateral effects compared to phytochemicals produced industrially (Gadano et al, 2006). Many of therapeutic activities attributed to certain plant drugs have no scientific support (Costa et al, 2008) and often times its ethnobotanic knowledge that directs phytochemical research of different metabolites and their biological effects.…”

“…On this basis, it is also important to determine the biological effects by in vivo bioassays. In this context, the alkaline version of Single Cell Gel Electrophoresis (SCGE) or Comet Assay has been used by many investigators to evaluate the genotoxicity of several chemicals in vitro and/or in vivo (Tice et al, 2000;Santos et al, 2008;Costa et al, 2008;Verschaeve and Van Staden, 2008;Moretti et al, 2013). The unique design of the comet assay provides direct determination of the single and double-strand DNA breaks, alkali-labile lesions and indirect excisions caused by repair enzymes in individual cells (Rojas et al, 1999;Kim et al, 2002;Collins et al, 2008;Trzeciak et al, 2008).…”