In Vitro Study of Neutrophil Apoptosis in Liver Cirrhosis.

Kusaba, Nobuhide; Kumashiro, Ryukichi; Ogata, Hideaki; Sata, Michio; Tanikawa, Kyuichi

doi:10.2169/internalmedicine.37.11

Cited by 36 publications

(21 citation statements)

References 26 publications

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“…In a study of Kusaba et al it was demonstrated that, in cirrhotic patients, exist an altered neutrophil kinetic and that an accelerated apoptosis is at the basis of neutropenia [10]. All these alterations can predispose to a more frequent bacterial and fungal infections in these patients.…”

Section: Discussionmentioning

confidence: 99%

Liver cirrhosis and rhino-orbital mucormycosis, a possible but rare association: description of a clinical case and literature review

Pellicelli

D’Ambrosio²,

Villani³

et al. 2009

Braz J Infect Dis

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Section: Discussionmentioning

confidence: 99%

Liver cirrhosis and rhino-orbital mucormycosis, a possible but rare association: description of a clinical case and literature review

Pellicelli

D’Ambrosio²,

Villani³

et al. 2009

Braz J Infect Dis

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“…Cirrhotic patients with advanced liver disease and portal hypertension frequently present with neutropenia. Although several mechanisms are implicated in the pathogenesis of this neutropenia, neutrophils from cirrhotic patients clearly have a shorter life span with accelerated apoptosis in vitro (29). G-CSF decreases apoptosis in neutrophils while enhancing motility and chemotaxis at low concentrations (13,37), thereby increasing neutrophil ability to migrate across vascular endothelium.…”

Section: Discussionmentioning

confidence: 99%

“…Neutrophil adhesion and migration across vascular endothelium are also essential steps in neutrophil activation that prime and modulate neutrophil activity in the inflammatory focus. Neutrophils from patients with liver cirrhosis show deficient phagocytosis, decreased intracellular bacterial killing, impaired neutrophil metabolic activity, increased apoptosis, and marked reduction in chemotaxis (29,35,43). Studies showing increased neutrophil adherence to nylon wool also suggest a possible defect in neutrophil-endothelial-cell interactions (2).…”

mentioning

confidence: 99%

Granulocyte Colony-Stimulating Factor Improves Deficient In Vitro Neutrophil Transendothelial Migration in Patients with Advanced Liver Disease

Fiuza

Salcedo

Clemente

et al. 2002

Clin Vaccine Immunol

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Bacterial infections are frequent complications in patients with liver cirrhosis. Cirrhotic patients present abnormalities in both innate and adaptive immune responses, including a deficient neutrophil recruitment to infected sites. The purpose of this study was to assess neutrophil-endothelium interactions in cirrhotic patients and evaluate the effects of G-CSF on this process. We studied neutrophil adhesion and transendothelial migration in 14 cirrhotic patients and 14 healthy controls. We also analyzed neutrophil expression of the adhesion molecules CD62L and CD11b in whole blood by flow cytometry. Cirrhotic patients expressed higher levels of CD11b than healthy controls, whereas CD62L expression was significantly lower, suggesting exposure of neutrophils to activating agents within the bloodstream. Neutrophils from cirrhotic patients showed increased adhesion to both resting and tumor necrosis factor alpha-stimulated microvascular endothelial cells and decreased transendothelial migration. Granulocyte colony-stimulating factor (G-CSF) (100 ng/ml) significantly enhanced neutrophil adhesion to microvascular endothelial cells in healthy controls but not in cirrhotic patients. G-CSF also significantly improved neutrophil transmigration in cirrhotic patients and healthy controls. In conclusion, cirrhotic patients exhibit increased neutrophil adhesion to microvascular endothelium and deficient transendothelial migration. G-CSF enhances neutrophil transendothelial migration in cirrhotic patients despite having no effect on neutrophil adhesion. Therefore, G-CSF may be able to increase neutrophil recruitment into infected sites in these patients

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“…Although splenic sequestration of leukocytes due to hypersplenism or serum hematopoietic progenitor inhibitory factors has been thought to be the main mechanism causing leukocytopenia [70,71] , a shortened neutrophil lifespan caused by increased apoptosis may also be responsible [72] . Lymphocytopenia is also often found in patients with liver cirrhosis, possibly due to malnutrition [73] .…”

Section: Leukocyte Abnormalitiesmentioning

confidence: 99%