In vitro study of the interactions of galactosylated thermo-responsive hydrogels with cells

Yang, Ning; Chen, Li; Yang, Miaokun; Bi, Sixin; He, Xiyun; Zhu, Zhengyan; Yu, Meili

doi:10.1016/j.carbpol.2011.12.036

Cited by 9 publications

(8 citation statements)

References 25 publications

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“…Moreover, Chung et al [24] and Park et al [25] demonstrated that the scaffolds immobilized by galactose retained a greater number of hepatocytes than those scaffolds which were unmodified or immobilized with galactose, due to specific interactions between hepatocytes and galactose moieties. Yang et al [26] and Wang et al [27] investigated the ability of hydrogels prepared with galactosylated acrylate (GAC) and poly (Nisopropylacrylamide) (NIPAAm) as scaffolds to proliferate hepatocytes and maintain the function of albumin and urea synthesis. They found that the cell adhesion and proliferation of hepatocytes occurred primarily on the surface of the hydrogels, suggesting that the incorporation of GAC containing galactose units could stimulate cell adsorption and growth, as compared with conventional PNIPAAm hydrogel.…”

Section: Introductionmentioning

confidence: 99%

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Zhao

Wang

et al. 2017

International Journal of Polymer Science

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Spherical biocarriers were prepared with lignin-carbohydrate complexes isolated from ginkgo (Ginkgo biloba L.) xylem. The specific surface and average pore size of the biocarriers were 17.15 m 2 g −1 and 21.59 nm, respectively. The carriers were stable in solution at pH 4.0∼9.5. Fourier transform infrared (FT-IR) spectrum indicated that the spherical carrier was composed of lignin and polysaccharides and had a typical lignin-carbohydrate complex (LCC) structure. The contents of galactose, lignin, and total sugar were 3.30%, 23.9%, and 64.62%, respectively, making the spherical biocarriers have good physical strength and compatible with hepatocytes. It was observed using a scanning electron microscopy (SEM) that liver cells adhered to the spherical biocarriers during culture. Cell counting indicated that the proliferation of liver cells in the experimental group was significantly higher than that of the control group. The albumin secretion (ALB) value and glucose consumption of the human hepatocytes were increased by 51.7% and 38.6%, respectively, by the fourth day when cultivated on the biocarriers. The results indicate that ginkgo LCC is very biocompatible and shows promise for the use as a biomaterial in the culture of human hepatocytes.

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Section: Introductionmentioning

confidence: 99%

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Zhao

Wang

et al. 2017

International Journal of Polymer Science

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“…H&E staining is one of the most commonly used observational staining methods for tissue and cell morphology as well as the pathological diagnosis and the clinical pathology diagnosis . Damages of general tissue and variation of cellular nuclei and cytoplasm could be displayed very clearly and colorfully by this method.…”

Section: Resultsmentioning

confidence: 99%

“…The MN ratio of HL‐7702 cells on the surface DVR‐50% with subcelluar pore size (∼10 μm) was the lowest one, suggesting that the surface with subcellular microporus structure surface had less mutagenic effect on the HL‐7702 cells. This can be attributed to physical properties as well as morphology of the biomaterials surface …”

Section: Resultsmentioning

confidence: 99%

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Fabrication of polystyrene microscale porous substrate and its effects on HL‐7702 cells behaviors

Yang

et al. 2013

J Biomedical Materials Res

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A novel polystyrene (PS) substrate with microscale porous structure was facilely fabricated by crystalline-controlled casting method using mixed solvent [N,N-dimethylformamide and ethyl alcohol (v/v)] based on the nonsolvent induced phase separation process. The substrate surfaces exhibited a bi-continuous microscale porous morphology with high porosity, large pore size and pore-pore connection structure. Moreover, behaviors of the normal human liver cell line (HL-7702) seeded on this substrate surface were carefully investigated. The results indicated that the cell adhesion, spread and cell-cell connection on the surface with subcellular pore size (∼ 10 μm) were similar to the cells proliferated on the flat PS surface. However, the number of HL-7702 cells proliferated on the PS microscale porous surface was higher than cells on the conventional PS flat surface, suggesting that the pore-pore structure was conducive to HL-7702 cell proliferation. Furthermore, hematoxylin and eosin staining and micronucleus test were performed. The results showed that fewer damages for nuclear and cytoplasm and less cell genotoxicity were caused by the microscale porous structure within the scope of pore size (∼ 10 μm) than that of the flat surface.

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“…In vitro and in vivo studies have been conducted to assess the biosynthetic activities of HL-7702 cells in order to explore the possibility to use this cell line for a liver support system. In a 7-day in vitro study done by Yang et al [ 40 ], the authors found that HL-7702 cells could keep their function of protein synthesis by culturing on thermoresponsive hydrogen. Albumin secretion continuously grew and the function of urea synthesis was significantly increased with in time delayed from (0.16 ± 0.02) μ mol/mL to (0.41 ± 0.04) μ mol/mL [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

Rhein ElicitsIn VitroCytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis through Mitochondria-Mediated Pathway

Bounda

Wang

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Objective. To study rhein-induced apoptosis signaling pathway and to investigate its molecular mechanisms in primary human hepatic cells. Results. Cell viability of HL-7702 cells treated with rhein showed significant decrease in dose-dependent manner. Following rhein treatment (25 μM, 50 μM, and 100 μM) for 12 h, the detection of apoptotic cells was significantly analyzed by flow cytometry and nuclear morphological changes by Hoechst 33258, respectively. Fatty degeneration studies showed upregulation level of the relevant hepatic markers (P < 0.01). Caspase activities expressed significant upregulation of caspase-3, caspase-9, and caspase-8. Moreover, apoptotic cells by rhein were significantly inhibited by Z-LEHD-FMK and Z-DEVD-FMK, caspase-9 inhibitor, and caspase-3 inhibitor, respectively. Overproduction of reactive oxygen species, lipid peroxidation, and loss of mitochondrial membrane potential were detected by fluorometry. Additionally, NAC, a ROS scavenger, significantly attenuated rhein-induced oxidative damage in HL-7702 cells. Furthermore, real-time qPCR results showed significant upregulation of p53, PUMA, Apaf-1, and Casp-9 and Casp-3 mRNA, with no significant changes of Fas and Cytochrome-c. Immunoblotting revealed significant Cytochrome-c release from mitochondria into cytosol and no change in Fas expression. Conclusion. Taken together, these observations suggested that rhein could induce apoptosis in HL-7702 cells via mitochondria-mediated signal pathway with involvement of oxidative stress mechanism.

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In vitro study of the interactions of galactosylated thermo-responsive hydrogels with cells

Cited by 9 publications

References 25 publications

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Lignin-Carbohydrate Complexes Based Spherical Biocarriers: Preparation, Characterization, and Biocompatibility

Fabrication of polystyrene microscale porous substrate and its effects on HL‐7702 cells behaviors

Rhein ElicitsIn VitroCytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis through Mitochondria-Mediated Pathway

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