In Vitro Study of the Adverse Effect of Nicotine and Physical Strain on Human Gingival Fibroblasts as a Model of the Healing of Wounds Commonly Found in the Military

Abstract

Initial treatment of HGF wounds with CMS resulted in faster wound repopulation regardless of nicotine presence. By day 6, wound healing of HGF exposed to both nicotine and CMS is delayed. These findings suggest that CMS and nicotine may affect fibroblasts and delay wound healing at other sites in the body as well.

Photobiomodulation Increases Viability in Full‐Thickness Grafts in Rats Submitted to Nicotine

“…VEGF plays an important role as a regulator in physiological vascular development, being a widely studied angiogenic factor; in tissues where angiogenesis is increased, its expression is considerably high . Evaluation of the presence of VEGF in the skin grafts points to higher values of VEGF related to greater viability of the skin graft, probably because of the influence on local angiogenesis . In the present study, the groups that were stimulated with PBM showed a higher amount of VEGF when compared with the other groups, demonstrating that the PBM can increase the amount of newly formed vessels in the tissue.…”

“…Smoking is detrimental to healing in both the acute and chronic phases, negatively influencing the fibroblastic activity, epithelialization, and immune response. Necrosis is three times higher in smokers, inhibiting the flow of oxygen to healing tissues (vasoconstriction), and elevated carboxyhemoglobin levels in the blood result in decreased oxygen tension in tissues .…”

Modifications in Human Oral Fibroblast Ultrastructure, Collagen Production, and Lysosomal Compartment in Response to Electronic Cigarette Fluids

“…Effects on HGFs of 1 mg/mL (i.e., 6.1 mM) nicotine, very close to the widely used 5 mM, 17 , 18 which has been demonstrated to be the concentration present in the saliva of smokers 19 was further investigated. Having demonstrated 12 , 20 that the high levels of ROS production and the occurrence of apoptosis are associated with exposure to e‐cigarette fluids, electron microscopy analyses were performed to determine the effects of these fluids on other features of cellular toxicity.…”

Efeito da estimulação elétrica de alta voltagem na integração de enxertos de pele total em ratos submetidos à ação da nicotina

“…A nicotina liberada do fumo acarreta um efeito vasoativo limitado na pele e tecido subcutâneo, o que claramente reduz o fluxo sanguíneo do tecido, comprometendo o metabolismo aeróbio, e é reconhecida como principal causadora de complicações pósoperatórias de feridas em indivíduos fumantes (Sørensen et al, 2009;Xanthoulea et al, 2013;Dinos et al, 2015;Pluvy et al, 2015), interferindo na síntese de colágeno, e prejudicando o processo de cicatrização (Jorgensen et al, 1998;Biondo-Simões et al, 2009).…”