In - vitro Study on α - amylase Inhibitory Activity of Selected Ethnobotanical Plant Extracts and its Herbal Formulations

Abstract

Background: Chronic disorder of carbohydrate, fat and protein metabolism characterized by increased fasting and postprandial blood sugar levels. Treatment of disorders in carbohydrate uptake required the inhibition of α-amylase plays a role in digestion of starch and glycogen, is considered a strategy for control the diabetes. Medicinal plants are an important source of phytochemical constituents with potential for inhibition of α-amylase and can be used as control of the diabetes. Objective: The present study deals with the evaluation of ethnomedicinal plants and their formulation for inhibitory activity on α-amylase enzyme. Material and Methods: The selected ethnobotanical plants of Gymnemasylvsetre, Cocciniagrandis, Tinosporacordifolia, Phyllanthus emblica, Aegelmarmelosusing the solvent of methanol, hexane, chloroform and ethyl acetate extraction and different herbal formulation possess significant inhibitory effect. Inhibitory effect further confirmation of glucose uptake by yeast cell and starch iodine assay of herbal formulation. Results:The formulation no. 3, 6, and 8 showed moderate α amylase inhibitory effect. This effect might be due to the presence of alkaloids, flavonoids, saponins, tannins and sterol, which was observed by preliminary phytochemical screening. Conclusion:This study showed that the herbal formulation of ethnobotanical plant extracts may be used to control hyperglycemia.

Inhibitory effect of leaf fractions of Setaria megaphylla on alpha amylase and alpha glucosidase activities in rats

“…Alpha-amylase and α-glucosidase inhibitions by plants extracts have been reported severally (Ishnava and Metisariya, 2018;Shirwaikar et al, 2005). Phytochemicals implicated as antidiabetic agents, do so possibly through α-amylase and glucosidase inhibition.…”

In Vitro Investigation and Evaluation of Novel Drug Based on Polyherbal Extract against Type 2 Diabetes

“…IC50 defines as the concentration at which inhibitor shows 50% of its inhibition activity. The percentage inhibition was calculated according to the following formula [23]:…”

Alpha-Amylase, Maltase and Sucrase Inhibitions by Aqueous Leaf Extracts of Anacardium occidentale (Anacardiacea) and Piliostigma reticulatum (Caesalpiniaceae) in Rats

“…protein precipitation agents forming insoluble complexes with proteins as well as acting as biological oxidants. 32 The presence of the polyphenolic compounds in the extracts of the two plants used in the study may suggests that their inhibitory potential on α-amylase and the membrane-bound intestinal α-glucosidase enzymes may be similar to the mechanism proposed generally for the polyphenolic compounds. However, further studies are necessary to confirm the mechanism(s) by which the extracts exhibit their blood glucose reducing effect.…”

“…Alpha-amylase and α-glucosidase inhibitions by plants extracts have been reported severally. 9,32,7 Phytochemicals implicated as anti-diabetic agents, do so possibly through α-amylase and glucosidase inhibition. The phytochemicals in question include flavonoids, saponins, tannins and terpenoids.…”