“In Vitro” Survival of Metaphase II Oocytes (MII) and Blastocysts After Vitrification in a Hemi-Straw (HS) System

Vanderzwalmen, Pierre; Bertin, G.; Debauche, C.; Standaart, V; Schoysman, E

doi:10.1016/s0015-0282(00)01358-3

Cited by 34 publications

(11 citation statements)

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“…To isolate the solution containing the oocytes or embryos against LN 2 during vitrification, various sealed container systems have been developed (11)(12)(13)(14). These methods do not seem to compromise oocyte-embryo survival or subsequent development, even though they determine a reduction in the rate of cooling (from >20,000 C/min to 200 C/min), which is contrary to the rapid cooling principle of vitrification (2,(14)(15)(16)(17)(18)(19). Recently, it has been demonstrated that specific ''open carriers'' for ultrarapid cooling, such as Cryotop (2) and Cryoleaf (3), generated very high survival, fertilization, pregnancy, and live births rates, especially with human oocytes (20,21), suggesting greater efficiency of these specific open tools compared with sealed systems.…”

Section: Discussionmentioning

confidence: 99%

Sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos

Parmegiani

Accorsi

Cognigni

et al. 2010

Fertility and Sterility

102

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Section: Discussionmentioning

confidence: 99%

Sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos

Parmegiani

Accorsi

Cognigni

et al. 2010

Fertility and Sterility

102

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“…The surface techniques ( Fig. 1) include EM grid (Steponkus et al 1990, Martino et al 1996, minimum drop size (MDS; Arav 1992, Arav & Zeron 1997, Cryotop (Hamawaki et al 1999, Kuwayama & Kato 2000, Cryoloop (Lane et al 1999a(Lane et al , 1999b, Hemi-straw (Vanderzwalmen et al 2000), solid surface (Dinnyes et al 2000), nylon mesh (Matsumoto et al 2001), Cryoleaf (Chian et al 2005), direct cover vitrification (Chen et al 2006), fiber plug (Muthukumar et al 2008), vitrification spatula (Tsang & Chow 2009), Cryo-E (Petyim et al 2009), plastic blade (Sugiyama et al 2010), and Vitri-Inga (Almodin et al 2010). To the tubing techniques (Fig.…”

Section: Germplasm Cryopreservationmentioning

confidence: 99%

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

2011

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Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans, cows, and mice. Two basic cryopreservation techniques rule the field -controlled-rate freezing, the first to be developed, and vitrification, which, in recent years, has gained a foothold. While much progress has been achieved in human medicine, the cattle industry, and in laboratory animals, this is far from being the case for most other mammals and even less so for other vertebrates. The major strides and obstacles in human and other vertebrate oocyte and embryo cryopreservation will be reviewed here.

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“…“Open” vitrification devices allow direct contact of the embryos with liquid nitrogen. Much of the early work establishing the efficacy of vitrification, and documenting the first live births, were performed using open carriers like the cryoloop [11-13], EM grids [14], Cryotop [15] and open pulled straws [16-19]. Despite successful use of “open” carriers for embryo vitrification, direct contact between the embryo and liquid nitrogen may not be desirable as there is a potential risk of cross-contamination between specimens or inadvertent exposure to contaminants present in the tank [20-22].…”

Section: Introductionmentioning

confidence: 99%

The new Rapid-i carrier is an effective system for human embryo vitrification at both the blastocyst and cleavage stage

Desai

Goldberg

Austin

et al. 2013

Reprod Biol Endocrinol

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BackgroundThe Rapid-i is a new FDA cleared closed carrier for embryo vitrification. The cooling rate of - 1220°C/min is far lower than that reported with open vitrification systems such as the cryoloop (−15,000°C/min). Little published data is currently available on this device. This study presents our initial clinical data, as well as live birth outcomes, with the Rapid-i. The efficacy of this device for the cryopreservation of cleavage, as well as blastocyst stage human embryos is also analyzed. We further compare outcomes to those achieved with the cryoloop, an “open” vitrification system routinely used in our laboratory.MethodsHuman embryos were vitrified at either the 8–10 cell stage or else the blastocyst stage. The vitrification protocol was: 7.5% DMSO/7.5% ethylene glycol (EG) (2–3 min) followed by incubation in 15% DMSO /15% EG (45 sec) before loading on the vitrification carrier. Cryoprotectant was removed during warming by sequential washes in 0.25 M and 0.125 M sucrose in culture medium. Clinical outcome data for frozen cycles between January 2011 and August 2012 were stratified according to carrier and cell stage. The student t-test and chi square test were used to compare results. P value of < 0.05 was considered significant.ResultsA total of 486 vitrified-warmed embryos were assessed and 92% of them were transferred. The clinical pregnancy rate (CPR) and implantation rate (IR) with Rapid-i vitrified blastocysts were 59% and 49%, versus 47% and 37%, respectively for cleavage stage embryos. This was not statistically different from results with the cryoloop vitrified blastocysts (CPR 46%, IR 38%) nor the cleavage stage vitrified embryos (CPR 49%, IR 35%). To date, there have been 31 deliveries of 34 healthy infants from Rapid-i vitrified embryos, with another 12 pregnancies still on-going.ConclusionsThe Rapid-i offers an excellent alternative to existing open vitrification devices for embryo cryopreservation at the 8–10 cell stage as well as the blastocyst stage. Use of this type of “closed” sealed system that prevents direct contact between the embryos and liquid nitrogen reduces the potential risk of sample cross-contamination or infection. These preliminary data and live birth outcomes have paved the way toward transitioning to a closed vitrification system in our own IVF program.

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“In Vitro” Survival of Metaphase II Oocytes (MII) and Blastocysts After Vitrification in a Hemi-Straw (HS) System

Cited by 34 publications

References 0 publications

Sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos

Sterilization of liquid nitrogen with ultraviolet irradiation for safe vitrification of human oocytes or embryos

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

The new Rapid-i carrier is an effective system for human embryo vitrification at both the blastocyst and cleavage stage

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