In vitro susceptibilities of the clinical isolate of Entamoeba histolytica to Euphorbia hirta (Euphorbiaceae) aqueous extract and fractions

Pechangou, Sylvain N.; Moundipa, Paul Fewou; Sehgal, Rakesh

doi:10.5897/ajmr2014.6929

Cited by 3 publications

(2 citation statements)

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“…These results are similar to those obtained with the aqueous leaf extract of C. variegatum against clinical isolates of E. histolytica trophozoites on polyxenic culture [18]. However, these activities are higher compared to those obtained with the aqueous extract of the same plant against HM1:IMSS strain of E. histolytica trophozoites on axenic culture [17], the aqueous leaf extract and fractions of E. hirta against the same isolates [26]. Forty plant extracts and their one hundred and twenty fractions were studied for the antiamoebic activity against clinical isolates of E. histolytica, and the results obtained were similar to those of the present finding [37].…”

Section: Discussionsupporting

confidence: 83%

Hydro-Alcoholic Leaf Extract and Fractions of <i>Codiaeum variegatum</i> (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Nsangou¹,

Fondjou²,

Mandou³

et al. 2023

AJPS

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Amoebiasis, classified as the third intestinal parasitic infection, represents a public health problem in low-income countries where hygiene and sanitation conditions are poor. With the resurgence of resistant pathogenic strains as well as ancestral considerations in developing countries such as Cameroon, many people rely on medicinal plants to treat a plethora of diseases. This work aimed to highlight the anti-amoebic and anti-oxidant potential of Codiaeum variegatum extracts and fractions. The anti-amoebic potential of C. variegatum was assayed on the polyxenic culture of the clinical isolates of E. histolytica. Then, the anti-oxidant potential of the ethanolic/hydroethanolic extracts and fractions was evaluated through DPPH radical scavenging, iron reduction (FRAP), lipid peroxidation inhibitory potential and total antioxidant capacity tests followed by the determination of phenolic compound and flavonoid content. It was found that the fractionation process decreased the amoebicidal activities of C. variegatum leaf extracts. However hydroethanolic extract (CI 50 : 10.08 ± 0.42, 5.18 ± 0.09, 5.18 ± 0.09 µg/mL respectively after 24, 48 and 72 hours) was more active than ethanolic extract (CI 50 : 15.59 ± 6.17; 9.61 ± 2.37; 6.26 ± 3.22 µg/mL respectively after 24, 48 and 72 hours). Interestingly, the activities of hydroethanolic extract were significantly non-different compared to metronidazole CI 50 : 8.42 ± 0.44, 6.45 ± 0.22 and 3.42 ± 0.33 µg/mL, respectively after 24, 48 and 72 hours). Ethanolic extract and EF5 showed higher Phenolic compound contents and higher antioxidant activity How to cite this paper: Nsangou

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Section: Discussionsupporting

confidence: 83%

Hydro-Alcoholic Leaf Extract and Fractions of <i>Codiaeum variegatum</i> (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Nsangou¹,

Fondjou²,

Mandou³

et al. 2023

AJPS

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“…Indeed, following the recurrence of toxicity and undesirable side effects of synthetic molecules used in the treatment of dysenteric diseases, the effect of three natural polyketides isolated from Phomopsis sp CAM212 was evaluated in this work on a polyxenic culture of E. histolytica. The polyxenic culture medium is a commonly used in vitro model for culturing clinical isolates of E. histolytica and performing anti-amoebic tests [27,28]. Indeed, the parasites grow in this environment in the presence of bacteria and yeasts that they use as protein sources; rice starch being the main energy source.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory and Antimicrobial Potential of Three Natural Polyketides Isolated from Endophytic Fungus Phomopsis sp CAM212 against to Dysenteric Causing Pathogens

Medoua,

Pechangou,

Enang II

et al. 2023

AJRIMPS

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Aims: The present work aimed to evaluate the anti-amoebic, antibacterial, and anti-inflammatory potential of three natural polyketides from Phomopsis sp. CAM212. Study Design: Clinical isolates of E.histolytica, E.coli ATCC25922 strain, primary peritoneal mouse macrophages and three polyketides were used. Places and Duration of Study: Laboratory of Pharmacology and Toxicology, Laboratory of Medical Microbiology, Faculty of Science, University of Yaounde 1 between May and December 2022. Methodology: During this work, we evaluated the ability of three natural polyketides from Phomopsis sp to inhibit the growth of germs responsible for amoebic and bacillary dysentery. First, the anti-amoebic activity was carried out on clinical isolates of E. histolytica in polyxenic culture. Subsequently, we evaluated the antibacterial potential on a strain of E. coli ATCC25922. Finally, the anti-inflammatory potentials were evaluated on a primary culture of SC activated macrophages through inhibition of nitric oxide (NO) production, activation of phosphatase alcaline (ALP) and inhibition of 5-lipoxygenase (5-LOX). Results: It emerges from this work that among compounds, phomopsinin B, presented the highest anti-amoebic potential (84.4 % inhibition after 72h) and the highest antibacterial potential (MIC=12.5µg/mL and MBC/MIC=2). Phomopsini A and phomopsini A acetate showed moderate anti amoebic and antibacterial potentials. However, all these activities remain lower than that of metronidazole and ciprofloxacin (90% of amoebic inhibition after 72h; MIC=0.72µg/mL and MBC/MIC=4). Subsequently, all tested compounds were nontoxic on primary macrophages. Phomopsinin B exhibited a great anti-inflammatory potential through the inhibition of NO production (IC50=1.72±0.91µg/mL); inihibition of 5-LOX activity (IC50=36.97±7.12µg/mL) and activation of ALP activity (IC50=0.13±0.01µg/mL) as compared to Baicalin the standard. The anti-inflammatory potential of phomopsinin A and phomopsinin A acetate were lower compared to baicalin. Conclusion: Ultimately, among compounds tested, phomopsinin B exhibited the best anti-amoebic, antibacterial and ant-inflammatory potential similar to the respective standards within the limits of the tests carried out.

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Improved traditional medicine for infectious disorders in Mali

Sanogo

Haïdara

Dénou

2022

Medicinal Plants as Anti-Infectives

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In vitro susceptibilities of the clinical isolate of Entamoeba histolytica to Euphorbia hirta (Euphorbiaceae) aqueous extract and fractions

Cited by 3 publications

References 18 publications

Hydro-Alcoholic Leaf Extract and Fractions of <i>Codiaeum variegatum</i> (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Hydro-Alcoholic Leaf Extract and Fractions of <i>Codiaeum variegatum</i> (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Anti-inflammatory and Antimicrobial Potential of Three Natural Polyketides Isolated from Endophytic Fungus Phomopsis sp CAM212 against to Dysenteric Causing Pathogens

Improved traditional medicine for infectious disorders in Mali

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In vitro susceptibilities of the clinical isolate of Entamoeba histolytica to Euphorbia hirta (Euphorbiaceae) aqueous extract and fractions

Cited by 3 publications

References 18 publications

Hydro-Alcoholic Leaf Extract and Fractions of &lt;i&gt;Codiaeum variegatum&lt;/i&gt; (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Hydro-Alcoholic Leaf Extract and Fractions of &lt;i&gt;Codiaeum variegatum&lt;/i&gt; (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Anti-inflammatory and Antimicrobial Potential of Three Natural Polyketides Isolated from Endophytic Fungus Phomopsis sp CAM212 against to Dysenteric Causing Pathogens

Improved traditional medicine for infectious disorders in Mali

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Hydro-Alcoholic Leaf Extract and Fractions of <i>Codiaeum variegatum</i> (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential

Hydro-Alcoholic Leaf Extract and Fractions of <i>Codiaeum variegatum</i> (var. Mollucanum) Exhibited an Improved Anti-Amoebic and Moderate Anti-Oxidant Potential