In vitro susceptibility of amphotericin-B, voriconazole and caspofungin against Candida guilliermondii biofilms, isolated from dentals units water pipes, under different growth phases

Mazari, Wissame; Boucherit-Otmani, Z.; Boucherit, K.

doi:10.1016/j.mycmed.2014.10.027

Cited by 5 publications

(6 citation statements)

References 36 publications

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“…Regarding the susceptibility of the Candida species to inhibition, the test compounds had lower MIC50 values than fluconazole and ketoconazole. However, if these results are compared with those reported in the literature for another class of azoles, such as voriconazole, we observe that the MIC50 for this antifungal in C. krusei is 0.25 [ 35 ], similar to that found in compound 4f , for C. glabrata and C. albicans MIC50 of 0.125 [ 43 ] and 1 [ 44 ] were found, respectively, observing that in C. glabrata , four compounds showed an MIC50 similar or better than voriconazole, while in C. albicans , the five derivatives showed better results. Regarding C. kefyr , four of the compounds showed MIC50 equivalent to that reported in voriconazole (0.125) [ 45 , 46 ], in C. guilliermondii [ 45 , 46 ], three of the compounds exhibited similar MIC50 (0.0625), while derivative 4j obtained a better value than the reference compound, and finally, for C. tropicalis [ 45 , 46 ], compound 4i showed a similar value (0.0312).…”

Section: Discussionsupporting

confidence: 70%

Synthesis, Molecular Docking, and Antimycotic Evaluation of Some 3-Acyl Imidazo[1,2-a]pyrimidines

Gómez-García

Andrade-Pavón²,

Campos-Aldrete

et al. 2018

Molecules

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A series of 3-benzoyl imidazo[1,2-a]pyrimidines, obtained from N-heteroarylformamidines in good yields, was tested in silico and in vitro for binding and inhibition of seven Candida species (Candida albicans (ATCC 10231), Candida dubliniensis (CD36), Candida glabrata (CBS138), Candida guilliermondii (ATCC 6260), Candida kefyr, Candida krusei (ATCC 6358) and Candida tropicalis (MYA-3404)). To predict binding mode and energy, each compound was docked in the active site of the lanosterol 14α-demethylase enzyme (CYP51), essential for fungal growth of Candida species. Antimycotic activity was evaluated as the 50% minimum inhibitory concentration (MIC50) for the test compounds and two reference drugs, ketoconazole and fluconazole. All test compounds had a better binding energy (range: −6.11 to −9.43 kcal/mol) than that found for the reference drugs (range: 48.93 to −6.16 kcal/mol). In general, the test compounds showed greater inhibitory activity of yeast growth than the reference drugs. Compounds 4j and 4f were the most active, indicating an important role in biological activity for the benzene ring with electron-withdrawing substituents. These compounds show the best MIC50 against C. guilliermondii and C. glabrata, respectively. The current findings suggest that the 3-benzoyl imidazo[1,2-a]pyrimidine derivatives, herein synthesized by an accessible methodology, are potential antifungal drugs.

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Section: Discussionsupporting

confidence: 70%

Synthesis, Molecular Docking, and Antimycotic Evaluation of Some 3-Acyl Imidazo[1,2-a]pyrimidines

Gómez-García

Andrade-Pavón²,

Campos-Aldrete

et al. 2018

Molecules

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“…In the planktonic form, this species was susceptible to antifungal agents; however, the mature biofilm of this species was resistant to antifungal drugs [12]. Souza-Gugelmin et al [43] demonstrated microbial contamination of the waterline of dental equipment due to formation of microbial biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…There are few literature studies on the fungal contamination of water in dental units and on the possible formation of biofilms [11, 12]. These issues are a reality in the routine of dental clinics and are worth monitoring if patients are to be successfully treated.…”

Section: Introductionmentioning

confidence: 99%

Risk of Fungal Infection to Dental Patients

Damasceno

Santos

Barbosa

et al. 2017

The Scientific World Journal

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Fungi can cause various diseases, and some pathogenic fungi have been detected in the water of dental equipment. This environment offers suitable conditions for fungal biofilms to emerge, which can facilitate mycological contamination. This study verified whether the water employed in the dental units of two dental clinics at the University of Franca was contaminated with fungi. This study also evaluated the ability of the detected fungi to form biofilms. The high-revving engine contained the largest average amount of fungi, 14.93 ± 18.18 CFU/mL. The main fungal species verified in this equipment belonged to the genera Aspergillus spp., Fusarium spp., Candida spp., and Rhodotorula spp. Among the isolated filamentous fungi, only one fungus of the genus Fusarium spp. did not form biofilms. As for yeasts, all the Candida spp. isolates grew as biofilm, but none of the Rhodotorula spp. isolates demonstrated this ability. Given that professionals and patients are often exposed to water and aerosols generated by the dental procedure, the several fungal species detected herein represent a potential risk especially to immunocompromised patients undergoing dental treatment. Therefore, frequent microbiological monitoring of the water employed in dental equipment is crucial to reduce the presence of contaminants.

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“…All yeasts recovered from the DCUWLs were selected to study their ability to form biofilms on the surfaces of microtitre plates. The biofilm‐forming species were retained to study their capacity to form biofilms against antifungal agents over time interval of 4, 24, 48 and 72 hours.…”

Section: Methodsmentioning

confidence: 99%

“…However, once inside the DCUWLs, microorganisms settle on the internal face and trigger a series of events leading to: colonisation, formation of microscopic colonies and, eventually, to biofilm.…”

Section: Introductionmentioning

confidence: 99%

Risk assessment for the spread of Candida sp. in dental chair unit waterlines using molecular techniques

Mazari

Boucherit-Otmani

Haci

et al. 2018

International Dental Journal

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In vitro susceptibility of amphotericin-B, voriconazole and caspofungin against Candida guilliermondii biofilms, isolated from dentals units water pipes, under different growth phases

Cited by 5 publications

References 36 publications

Synthesis, Molecular Docking, and Antimycotic Evaluation of Some 3-Acyl Imidazo[1,2-a]pyrimidines

Synthesis, Molecular Docking, and Antimycotic Evaluation of Some 3-Acyl Imidazo[1,2-a]pyrimidines

Risk of Fungal Infection to Dental Patients

Risk assessment for the spread of Candida sp. in dental chair unit waterlines using molecular techniques

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