In vitro susceptibility of European human Borrelia burgdorferi sensu stricto strains to antimicrobial agents

Veinović, Gorana; Cerar, Tjaša; Strle, Franc; Lotrič‐Furlan, Stanka; Maraspin, Vera; Cimperman, J; Ružić‐Sabljić, Eva

doi:10.1016/j.ijantimicag.2012.11.016

Cited by 20 publications

(31 citation statements)

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“…In this study, we describe, to our knowledge for the first time, the in vitro susceptibilities of B. miyamotoi to the antibiotics most commonly used in the treatment of HTBRF and Lyme borreliosis. Microdilution-based methods have been extensively studied by others and have been shown to be robust methods for standardized testing of the antimicrobial susceptibility of Borrelia species (18,20,21). Using similar methods, we demonstrated that B. miyamotoi strains HT31 and LB-2001 are susceptible to azithromycin, doxycycline, and ceftriaxone but-compared to the B. burgdorferi sensu lato strains tested-resistant to amoxicillin in vitro.…”

Section: Discussionmentioning

confidence: 78%

“…The antimicrobial susceptibility of B. miyamotoi has not yet been elucidated, due to difficulties with the cultivation of B. miyamotoi spirochetes in vitro, which has been reported only recently (13)(14)(15). In contrast, the in vitro susceptibilities of the causative agents of Lyme borreliosis and several RF Borrelia spirochetes have been extensively studied (16)(17)(18)(19). The antimicrobial susceptibility of spirochetes is conventionally shown as MICsmeasured either by direct (dark-field) microscopy or by colorimetric assays-or measurement of the minimal bactericidal concentrations (MBCs) after 72 h of exposure to antibiotics (18,20,21).…”

mentioning

confidence: 99%

“…In contrast, the in vitro susceptibilities of the causative agents of Lyme borreliosis and several RF Borrelia spirochetes have been extensively studied (16)(17)(18)(19). The antimicrobial susceptibility of spirochetes is conventionally shown as MICsmeasured either by direct (dark-field) microscopy or by colorimetric assays-or measurement of the minimal bactericidal concentrations (MBCs) after 72 h of exposure to antibiotics (18,20,21). As part of the current study, we aimed to determine the in vitro antibiotic susceptibility of B. miyamotoi in comparison to those of B. burgdorferi sensu lato and B. hermsii, by determining the MICs and MBCs of the antibiotics most commonly used in the treatment of Lyme borreliosis by both dark-field microscopy and colorimetric assays.…”

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confidence: 99%

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In Vitro Susceptibility of the Relapsing-Fever Spirochete Borrelia miyamotoi to Antimicrobial Agents

Koetsveld

Draga

Wagemakers

et al. 2017

Antimicrob Agents Chemother

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Hard-tick-borne relapsing fever (HTBRF) is an emerging infectious disease throughout the temperate zone caused by the relapsing-fever spirochete Borrelia miyamotoi. Antibiotic treatment of HTBRF is empirically based on the treatment of Lyme borreliosis; however, the antibiotic susceptibility of B. miyamotoi has not been studied to date. Thus, we set out to determine the in vitro antimicrobial susceptibility of B. miyamotoi. A microdilution method with 96-well microtiter plates was used to determine the antibiotic susceptibilities of two B. miyamotoi strains isolated on two different continents (Asia and North America), two Borrelia burgdorferi sensu lato strains, and one Borrelia hermsii isolate for purposes of comparison. The MIC and minimal bactericidal concentration (MBC) were determined by both microscopy and colorimetric assays. We were able to show that relative to the B. burgdorferi sensu lato isolates, both B. miyamotoi strains and B. hermsii demonstrated greater susceptibility to doxycycline and azithromycin, equal susceptibility to ceftriaxone, and resistance to amoxicillin in vitro. The MIC and MBC of amoxicillin for B. miyamotoi evaluated by microscopy were 16 to 32 mg/liter and 32 to 128 mg/liter, respectively. Since B. miyamotoi is susceptible to doxycycline, azithromycin, and ceftriaxone in vitro, our data suggest that these antibiotics can be used for the treatment of HTBRF. Oral amoxicillin is currently used as an alternative for the treatment of HTBRF; however, since we found that the B. miyamotoi strains tested were resistant to amoxicillin in vitro, this issue warrants further study. KEYWORDS hard-tick-borne relapsing fever, relapsing-fever borrelia, Borrelia miyamotoi, Borrelia miyamotoi disease, antibiotic susceptibility, antimicrobials T he relapsing-fever spirochete Borrelia miyamotoi was first described in Japan in 1995 (1). While it is phylogenetically closely related to other relapsing-fever (RF) spirochetes, such as Borrelia hermsii, Borrelia turicatae (transmitted by soft ticks), and Borrelia recurrentis (transmitted by body lice), it is transmitted by hard-bodied Ixodes ticks across the temperate zone (2). Ixodes ticks concomitantly transmit spirochetes belonging to the Borrelia burgdorferi sensu lato group, which are known to cause Lyme borreliosis (3). The incidence of B. miyamotoi in Ixodes ticks is lower than that of B. burgdorferi sensu lato, with infection rates ranging from 0 to 15.4% in the United States and as high as 4% in Europe and Japan (2, 4, 5). RF Borrelia spirochetes cause a variety of diseases, which are characterized by episodes of high fever separated by periods of relative well-being. The clinical presentation of disease caused by B. miyamotoi, however, appears to differ from that of classical RF, since relapsing-fever episodes have been observed only in 10% of patients infected with B. miyamotoi, and their levels of spirochetemia are calculated to be low (6, 7). Since most patients with B. miyamotoi infection are usually treated with antibiotics, this ...

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Section: Discussionmentioning

confidence: 78%

mentioning

confidence: 99%

mentioning

confidence: 99%

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In Vitro Susceptibility of the Relapsing-Fever Spirochete Borrelia miyamotoi to Antimicrobial Agents

Koetsveld

Draga

Wagemakers

et al. 2017

Antimicrob Agents Chemother

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“…To nullify the effect of extract color, optical density (O.D.) of the extract control was subtracted from treated columns corresponding to extract treated [21]. Percent inhibition was calculated using following equation: Minimum inhibition concentrations (MICs) was calculated based on optical density recorded with a spectrophotometer (SpectraMax Plus 384 , Molecular Devices Corporation, USA) at 530 nm after 96 h of incubation at 30 ± 2 °C (Figure 1).…”

Section: Determination Of Fungistatic Concentrationmentioning

confidence: 99%

Antidermatophytic Activity of the Fruticose Lichen <em>Usnea orientalis</em>

Pathak

Dikshit

Upreti

2016

Preprint

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Abstract:In the present study, the new biological sources in the form of lichen Usnea orientalis Motyka was screened for its antidermatophytic potential. Six species of dermatophytes were chosen on the basis of their prevalence for antidermatophytic assays, and the Clinical Laboratory Standard Institute (CLSI)-recommended broth microdilution procedure was used to detect the efficacy of extract against dermatophytes. Thin layer chromatography of lichen extracts reveals the presence of two secondary metabolites viz. salazinic acid and usnic acid. U. orientalis extract exhibited promising antidermatophytic activity against all tested pathogens. Amongst all tested pathogens, E. floccosum exhibited most susceptibility towards extract, whereas T. mentagrophytes exhibited the least susceptibility. Topical application of U. orientalis extract might be helpful in the cure of dermal infections.

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“…Mnoga in vitro istraživanja su pokazala da su borelije koje uzrokuju lajmsku boreliozu osetljive na širok spektar antimikrobnih agenasa uključujući amoksicilin, azitromicin, ceftriakson, cefuroksim, doksiciklin, penicilin G, itd. (47)(48)(49)(50)(51)(52). In vitro testiranje osetljivosti B. burgdorferi sensu lato na antibiotike uvek je bilo ograničeno usled: malog broja izolata na raspolaganju u pojedinim studijama, retkog izvođenja testa na sojevima neposredno izolovanih od pacijenata koji su rezistentni na terapiju i/ili zbog toga što sama metodologija nije standardizovana.…”

Section: Terapija Lajmske Boreliozeunclassified

Lyme borreliosis: Epidemiology, clinical manifestations and treatment

Veinović¹,

Stojić-Vukanić²,

Antić-Stanković³

2015

Arh farmaciju

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Lajmska borelioza je multisistemska bolest koju karakteriše širok spektar kliničkih manifestacija.U Evropi je primarno prouzrokovana vrstama B. afzelii, B. garinii i B. burgdorferi sensu stricto, ali Borrelia spielmanii, Borrelia valaisiana, Borrelia lusitaniae and Borrelia bissettii se takođe navode kao retki ili potencijalni uzročnici oboljenja kod ljudi. U Severnoj Americi, kao jedini humani patogen, dokazana je vrsta B. burgdorferi sensu stricto. Najčešća klinička manifestacija lajmske borelioze je kožna lezija, erythema migrans, koja može da prođe spontano, bez terapije antibioticima. Međutim, patogen se može širiti do drugih delova kože i organa i tako prouzrokovati teže manifestacije kako na koži, tako i na nervnom sistemu, zglobovima i/ili srcu, pri čemu je terapija antibioticima neophodna. Najčešće korišćeni antibiotici u terapiji lajmske borelioze su: amoksicilin, ceftriakson, cefuroksim, doksiciklin i azitromicin.

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In vitro susceptibility of European human Borrelia burgdorferi sensu stricto strains to antimicrobial agents

Cited by 20 publications

References 11 publications

In Vitro Susceptibility of the Relapsing-Fever Spirochete Borrelia miyamotoi to Antimicrobial Agents

In Vitro Susceptibility of the Relapsing-Fever Spirochete Borrelia miyamotoi to Antimicrobial Agents

Antidermatophytic Activity of the Fruticose Lichen <em>Usnea orientalis</em>

Lyme borreliosis: Epidemiology, clinical manifestations and treatment

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