In Vitro Susceptibility of Ornithobacterium rhinotracheale to Several Antimicrobial Drugs

Soriano, V. E.; Vera, Nadia; Salado, C. R.; Fernandez, Rachel; Blackall, P. J.

doi:10.1637/0005-2086(2003)047[0476:ivsoor]2.0.co;2

Cited by 30 publications

(20 citation statements)

References 14 publications

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“…At the same time, most authors found O. rhinotracheale strains to be resistant to gentamicin (Ak and Turan, 2001;Malik et al, 2003a;Soriano et al, 2003;Murthy et al, 2008) and sulphamethoxazole-trimethoprim (Malik et al, 2003a;Tsai and Huang, 2006;Mohd-Zain et al, 2008;Murthy et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Varga et al (2001) observed low MICs to erythromycin; however, the drug inhibited the growth of O. rhinotracheale strains only in high concentrations or not at all in the Netherlands (van Veen et al, 2001). Similarly, the susceptibility of Mexican strains to amoxicillin ranged from 16 µg/ml to 128 µg/ml with only one susceptible isolate at 2 µg/ml (Soriano et al, 2003), and all Dutch isolates were resistant, too (64 µg/ml) (van Veen et al, 2001) whereas elsewhere a wider range (≤ 0.06 µg/ml to ≥ 64 µg/ml) was reported (Varga et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial susceptibility of Bordetella Avium and Ornithobacterium Rhinotracheale strains from wild and domesticated birds in Hungary

Szabó

Wehmann

Magyar

2015

Acta Veterinaria Hungarica

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The antimicrobial susceptibility of 19 Bordetella avium and 36 Ornithobacterium rhinotracheale strains was tested by the Kirby-Bauer disk diffusion method, and the minimal inhibitory concentrations (MIC) of amoxicillin, doxycycline and erythromycin were also determined. Most O. rhinotracheale strains were resistant to nalidixic acid, sulphamethoxazole-trimethoprim and gentamicin, and were susceptible to ampicillin, chloramphenicol, spectinomycin and tilmicosin. All B. avium strains were resistant to ceftiofur and lincomycin and susceptible to doxycycline, gentamicin, polymyxin B, spectinomycin and sulphonamides. The MICs ranged widely for all three antibiotics tested against O. rhinotracheale strains, from 0.12 µg/ml to 32 µg/ml for amoxicillin and erythromycin, and from 0.6 µg/ml to 32 µg/ml for doxycycline. For B. avium isolates, the MIC values ranged from ≤ 0.03 µg/ml to 1 µg/ml for amoxicillin, from ≤ 0.03 µg/ml to 0.12 µg/ml for doxycycline and from 8 µg/ml to 16 µg/ml for erythromycin. These findings support the idea that the use of antibiotics in a region or a farm may affect antimicrobial resistance and underline the need for prudent application of antibiotic therapy based on proper antimicrobial susceptibility testing.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Antimicrobial susceptibility of Bordetella Avium and Ornithobacterium Rhinotracheale strains from wild and domesticated birds in Hungary

Szabó

Wehmann

Magyar

2015

Acta Veterinaria Hungarica

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“…Subsequently, pairs of O. rhinotracheale isolates (stable O. rhinotracheale SCVs and their WT counterparts) that showed different patterns of susceptibility to certain antibiotics were further tested to identify the MIC values for those antibiotics, as described previously (36). Therefore, O. rhinotracheale isolates were grown on TSB supplemented with 5% FBS and 2% IsoVitaleX Enrichment at 37°C under microaerobic conditions (see below), and standard microdilution procedures were conducted using the same medium and conditions to determine MIC values.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of Small-Colony Variants of Ornithobacterium rhinotracheale

et al. 2013

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bOrnithobacterium rhinotracheale is a Gram-negative bacterium associated with respiratory diseases in many avian species, with worldwide distribution, and it causes significant economic loss to the poultry industry. In this study, the isolation and characterization of O. rhinotracheale small-colony variants (SCVs) are described for the first time. O. rhinotracheale isolates (n ‫؍‬ 27) were recovered from tracheal samples (n ‫؍‬ 321) collected from different avian species with clinical signs of respiratory disease. Of the 27 O. rhinotracheale isolates, 21 (77.8%) showed SCVs in their primary cultures. Five O. rhinotracheale SCV isolates showed high levels of stability and were chosen for further characterization with their wildtype (WT) isolates. Stable O. rhinotracheale SCVs were oxidase negative, while their WT isolates were positive. Growth curves for stable O. rhinotracheale SCVs indicated lower growth rates and longer lag phases than for their WT isolates. Furthermore, it was possible to increase the efficacy of the broth medium in supporting the growth of O. rhinotracheale WT isolates by supplementing it with 5% fetal bovine serum (FBS) and 2% IsoVitaleX Enrichment. Antibiotic susceptibility tests showed that O. rhinotracheale SCVs had higher MIC values than their WT isolates. This study suggests that successful antibiotic treatment of respiratory diseases associated with O. rhinotracheale must take into consideration the resistance patterns of O. rhinotracheale SCVs. Intracellular persistence in murine RAW 264.7 macrophages revealed that O. rhinotracheale SCV28 had higher survival rates than its WT isolate. Finally, small-colony variants may be important contributors to the pathogenesis of O. rhinotracheale.

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“…Furthermore, other infectious agents of the respiratory tract such as Escherichia coli (24), Bordetella avium (7), and Newcastle diseases (ND) virus (31) have a triggering effect on the manifestation of O. rhinotracheale infection. Treatment of O. rhinotracheale infection is becoming more and more difficult, as most isolates have acquired resistance against the regularly used antibiotics (8,17,29). Therefore, administration of a suitable vaccine inducing protective immunity against O. rhinotracheale infection is a good solution.…”

mentioning

confidence: 99%

Successful Selection of Cross-Protective Vaccine Candidates for Ornithobacterium rhinotracheale Infection

Schuijffel¹,

Empel²,

Pennings³

et al. 2005

Infect Immun

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Ornithobacterium rhinotracheale is a bacterial pathogen known for causing respiratory disease in poultry. In this study, we demonstrate for the first time that cross-protective immunity against different O. rhinotracheale serotypes can be induced by live vaccination. Sera from these live-vaccinated and cross-protected birds were used to identify new vaccine targets by screening an O. rhinotracheale expression library. Out of 20,000 screened plaques, a total of 30 cross-reactive clones were selected for further analysis. Western blot analysis and DNA sequencing identified eight different open reading frames. The genes encoding the eight cross-reactive antigens were amplified, cloned in an expression vector, and expressed in Escherichia coli. Purified recombinant proteins with a molecular mass ranging from 35.9 kDa to 62.9 kDa were mixed and tested as a subunit vaccine for (cross-)protection against challenge with homologous and heterologous O. rhinotracheale serotypes in chickens. Subunit vaccination resulted in the production of antibodies reactive to the recombinant proteins on Western blot, and this eight-valent vaccine conferred both homologous and heterologous protection against O. rhinotracheale challenge in chickens.

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In Vitro Susceptibility of Ornithobacterium rhinotracheale to Several Antimicrobial Drugs

Cited by 30 publications

References 14 publications

Antimicrobial susceptibility of Bordetella Avium and Ornithobacterium Rhinotracheale strains from wild and domesticated birds in Hungary

Antimicrobial susceptibility of Bordetella Avium and Ornithobacterium Rhinotracheale strains from wild and domesticated birds in Hungary

Isolation and Characterization of Small-Colony Variants of Ornithobacterium rhinotracheale

Successful Selection of Cross-Protective Vaccine Candidates for Ornithobacterium rhinotracheale Infection

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