In Vitro Synergy of Daptomycin plus Rifampin against
            <i>Enterococcus faecium</i>
            Resistant to both Linezolid andVancomycin

Pankey, George A.; Ashcraft, Deborah; Patel, Nalini

doi:10.1128/aac.49.12.5166-5168.2005

Cited by 79 publications

(51 citation statements)

References 18 publications

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“…Their primary finding was that TKA demonstrated consistent synergism at various concentrations of antimicrobial, while checkerboard testing could only demonstrate indifference (13). These two studies reflect the consensus of others who have compared synergy methods for different antimicrobial combinations and bacterial species and all found the same thing, that no two synergy methods produce comparable results (14)(15)(16)(17). However, in some circumstances, Ͼ90% correlation between Etest and checkerboard testing has been observed (18).…”

Section: Comparison Of Synergy Testing Methodsmentioning

confidence: 86%

When Does 2 Plus 2 Equal 5? A Review of Antimicrobial Synergy Testing

2014

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In this age of emerging antibiotic resistance, limited therapeutic options exist for treating multidrug-resistant organisms. Combination therapy is commonly employed to manage these infections despite little laboratory guidance as to the efficacy of this approach. Synergy testing methods have been used to assess the interaction of antibiotic combinations in vitro. This review will discuss the four primary methods used to assess synergy, as well as the data that exist for testing of cystic fibrosis. In the final analysis, this review concludes that there is not enough evidence to endorse synergy testing for routine clinical use.

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Section: Comparison Of Synergy Testing Methodsmentioning

confidence: 86%

When Does 2 Plus 2 Equal 5? A Review of Antimicrobial Synergy Testing

2014

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“…Rifampin has shown good activity against Staphylococcus aureus biofilm (13) and is recommended for treatment of staphylococcal prosthetic valve endocarditis (14). However, it has not been extensively studied for Enterococcus species infections, although some promising results have been reported (10,18). As optimal therapy for the treatment of E. hirae infection remains unknown and valve replacement was contraindicated for our patient, we hypothesized that the addition of rifampin might optimize antibiotic therapy and reduce the risk of relapse.…”

Section: Case Reportmentioning

confidence: 99%

Relapse of Enterococcus hirae Prosthetic Valve Endocarditis

et al. 2011

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Enterococcus hirae, a Gram-positive bacterium, is a rare isolate in clinical specimens. We report an unusual case of a relapse of prosthetic valve endocarditis due to E. hirae 6 months after the initial episode. Clonal relationship was proven by genomic analysis. CASE REPORTIn April 2008, a 78 year-old woman presented with a 5-month history of fever, generalized weakness, and a 7-kg weight loss. She had a history of diabetes mellitus and hypertension and had undergone in 2001 an aortic valve replacement with a bioprosthetic valve. On admission, her temperature was 38°C, and cardiac examination revealed a 3/6 systolic murmur. Transthoracic and transesophageal echocardiographies showed no evidence of endocarditis.Five blood cultures yielded colonies of Gram-positive cocci with a morphology typical of Streptococcus. On blood agar culture, colonies were circular and smooth and reached 0.2 to 0.5 mm after 24 to 48 h of incubation in the presence of 5% CO 2 . The strain was identified by phenotypic determination and genetic analysis. The phenotypic identification was based upon colony and Gram stain morphologies and a negative catalase reaction. The cocci reacted with both Lancefield group F and D antisera (Streptex; Diamondial, Sees, France). The biochemical identification remained difficult even by a semiautomated system. Accurate identification to the species level was not possible with the IDGP N052 card (bioMérieux, Marcy l'Etoile, France) due to low discrimination (50.52% for Enterococcus durans and 49.48% for Enterococcus faecium). The Rapid ID 32 Strep identification system (bioMérieux) failed, producing an unacceptable profile for Enterococcus gallinarum with the current database.The strain was correctly identified to the species level as Enterococcus hirae by genetic methods using 16S rRNA and sodA int gene sequencing (where "int" represents "internal") as previously described (5, 12). Sequence analysis of this strain yielded 99.81% and 99.76% identities with the sequences of the 16S rRNA gene (GenBank accession no. AB362598) of type strain of E. hirae and the sodA gene (GenBank accession no. AJ387916) of E. hirae, respectively. As the growth was deficient, antibiotic susceptibility testing was performed with an agar diffusion technique using a Mueller-Hinton agar supplemented with 5% horse blood (bioMérieux). The strain was susceptible to amoxicillin (MIC, 0.032 g/ml, as measured by the Etest technique), moxifloxacin, vancomycin, teicoplanin, erythromycin, and rifampin but resistant to clindamycin, tetracycline, and fosfomycin and exhibited low-level resistance to streptomycin, kanamycin, and gentamicin.Despite our patient's normal echocardiography, a diagnosis of possible infective endocarditis was established (Table 1), and intravenous amoxicillin (200 mg per kg of body weight per day) and gentamicin (3 mg per kg per day) were initiated. After 2 weeks, gentamicin was discontinued and rifampin (20 mg per kg per day) was initiated. Antimicrobial treatment was administered for 6 weeks. The patient improved c...

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“…Örneğin, yapılan bir çalışmada telavansin ve daptomisin ile rifampin kombinasyonlarının in-vitro şartlarda linezolide ve vankomisine dirençli enterokok suşlarına etkisi araştırılmış, telavansin/rifampin kombinasyonunda %83 (20/24), daptomisin/rifampin kombinasyonununda ise %88 (21/24) oranında da sinerjist etki saptanmıştır (9) . Çalışma E-test ile yapılmış ve sinerjist fraksiyonel inhibitör konsantrasyon indeksi (FİKİ) ≤0.5; indifferens etki >0.5-4 ve antagonist etki >4 kriterine göre değerlendiril-miştir.…”

Section: Introductionunclassified

“…Aynı kanallardan çok fazla rifampin hücre içine girerek RNA polimeraz enziminin aktivitesini engeller ve hızlı hücre ölümü gerçekleşir (12) . Sonuç olarak bu çalışma-da, daptomisin ve telavansinin, rifampin ile kombinasyonlarının yüksek oranda sinerjist etkili bulunduğu, in-vivo etkileşimin de belirlenmesine gereksinim olduğu bildirilmektedir (9) .…”

Section: Introductionunclassified

Antibiotic Combinations and Synergistic Interactions

Aktaş¹

2015

TMCD

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Derleme ÖZET Antibiotic combination studies allow exploration of different molecular targets of individual agents and thereby broaden the spectrum of action. The synergistic effect of combinations occurs through inhibition of different targets through different mechanisms or by inhibiting targets in the same way or by inhibiting the same target in different ways Türk Mikrobiyol Cem Derg 44(2):47-55, 2014 doi:10.5222/TMCD.2014 Gülseren AKTAŞ İstanbul Üniversitesi İstanbul Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı Antibiyotik Kombinasyonları ve Sinerjistik Etkileşimleri GİRİŞ Antibiyotikler ilk ortaya çıktığı zamanlarda henüz direnç sorunu yoktu ve bu nedenle başa-rıyla kullanılmışlardı. Günümüzde, tüm dünyada her geçen gün artan dirençli patojenler, yeni antimikrobiyallere olan gereksinimi arttırmıştır. Direnç sorunu, bazı patojen suşların veya bazı hastane bölümlerinin sorunu olmaktan çıkmış ve hemen tüm majör bakteriyel patojenlerde ve acil bakım, yoğun bakım üniteleri gibi tedavi ünite-lerinde ve hatta toplum kökenli enfeksiyonlarda görülür olmuştur (1) .Çoğul antibiyotik dirençli gram-pozitif patojenler ilk defa 1980'lerde ortaya çıkmıştır (2) .

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In Vitro Synergy of Daptomycin plus Rifampin against Enterococcus faecium Resistant to both Linezolid andVancomycin

Cited by 79 publications

References 18 publications

When Does 2 Plus 2 Equal 5? A Review of Antimicrobial Synergy Testing

When Does 2 Plus 2 Equal 5? A Review of Antimicrobial Synergy Testing

Relapse of Enterococcus hirae Prosthetic Valve Endocarditis

Antibiotic Combinations and Synergistic Interactions

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