In vitro tissue oxygen tensions in the rabbit aortic arch

Jurrus, Eric; Weiss, Harold S.

doi:10.1016/0021-9150(77)90172-1

Cited by 47 publications

(43 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our profiles in this region were continuous, rising progressively to that of arterial blood as the wall was crossed from adventitia to lumen. Near the intima, our findings were similar to in vitro studies of excised arterial wall reported by Schneidermlani et al (10) and by Jurrus and Weiss (11).…”

Section: Discussionsupporting

confidence: 93%

“…We discuss here technical differ-ences which may have caused our profiles to resemble previous profiles obtained in vitro, and not those in vivo. We used the recessed-tip glass microcathode of the Whalen type as did Jurrus and Weiss (11) Oxygeni con1sum71ptiO11, wall structure, atnd stress.…”

Section: Discussionmentioning

confidence: 99%

“…To make an approximate comparison, we have assumed tissue weight of 1.06 g/ml, with a water content of 75% (26,27). Using these values when necessary, representative results are 20 nlo/cm3-s wet tissue in human umbilical arteries (28); 50 nlo/cmn-s in bovine mesenteric veins (29); 57 and 71 nlO/cm3-s in rabbit aortas (5,11); and 110-230 nlO/cm113-s in resting and stimllulated dog femoral artery segments (30). Our meani value of 99 and range of 56-142 nlo/CM3-s appear reasonable with respect to these respirometer studies.…”

Section: Discussionmentioning

confidence: 99%

“…It clearly suggests a transport barrier for oxygen in this region in relation to the level of medial oxygen consumption. Subsequent in vitro measurements of wall oxygenation have not confirmed the presence of such a lbarrier (10,11). No transport theory modeling was uised in the in vivo studies of Moss et al (8) or Niinikoski et al (9) and in vivo oxygen consumptions were not calculated.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In vivo oxygen transport in the normal rabbit femoral arterial wall.

Crawford¹,

Back²,

Cole³

1980

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vivo oxygen transport in the normal rabbit femoral arterial wall.

Crawford¹,

Back²,

Cole³

1980

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…Later in the disease process, increased diffusion distances, attributable to intimal thickening as well as a high consumption of oxygen 8 and glucose 9,10 by foam cells, could additionally aggravate energy metabolic predicaments and contribute to the development of a necrotic core. Supportive of this hypothesis, hypoxic zones have been demonstrated in situ in the normal pig arterial wall 11 as well as in vitro [12][13][14] and in vivo 15 in rabbit atherosclerotic plaques. However, ATP production may be maintained under hypoxic conditions via anaerobic breakdown of glucose and glycogen, but this could lead to a potentially harmful accumulation of lactate.…”

mentioning

confidence: 74%

Mapping of ATP, Glucose, Glycogen, and Lactate Concentrations Within the Arterial Wall

Levin

Leppänen

Evaldsson

et al. 2003

ATVB

View full text Add to dashboard Cite

Objective-In large-and medium-sized arteries, the diffusion distances for oxygen and nutrients are long. This has been suggested to make these vessels prone to develop local energy metabolic deficiencies that could contribute to atherogenesis. To validate this hypothesis, we introduced a new method to measure energy metabolites within the arterial wall at high spatial resolution. Methods and Results-Bioluminescence imaging was used to quantify local metabolite concentrations in cryosections of snap frozen (in vivo) and incubated pig carotid artery rings. Incubation at hypoxia resulted in increased lactate concentrations, whereas ATP, glucose, and glycogen concentrations were decreased, especially in the mid media, where concentrations of these metabolites were close to zero. In snap frozen arteries, glycogen concentrations were markedly higher in deep layers of the media than toward the lumen. ATP, glucose, and lactate were more homogenously distributed. Key Words: atherosclerosis Ⅲ hypoxia Ⅲ bioluminescence Ⅲ energy metabolism Ⅲ imaging T he arterial wall is supplied with oxygen and nutrients by diffusion and convection from luminal blood and from vasa vasorum in the adventitia and outer media. Metabolic waste products are removed by diffusion in the opposite direction. Diffusion distances in large-and medium-sized arteries are often close to, or even exceed, the 100 to 200 m that is frequently stated as the diffusion limit for oxygen. 1-4 Based on these facts, it has been suggested that the arterial wall is predisposed to develop local energy metabolic disturbances and that this is a key factor both in the initiation and progression of atherosclerosis. 1 In atherosclerosis-prone areas, both insufficient transport 5,6 and increased consumption of metabolites 7 could contribute to local disturbances. Later in the disease process, increased diffusion distances, attributable to intimal thickening as well as a high consumption of oxygen 8 and glucose 9,10 by foam cells, could additionally aggravate energy metabolic predicaments and contribute to the development of a necrotic core. Supportive of this hypothesis, hypoxic zones have been demonstrated in situ in the normal pig arterial wall 11 as well as in vitro 12-14 and in vivo 15 in rabbit atherosclerotic plaques. However, ATP production may be maintained under hypoxic conditions via anaerobic breakdown of glucose and glycogen, but this could lead to a potentially harmful accumulation of lactate. Therefore, other aspects of energy metabolism besides the supply of oxygen need to be analyzed. To the best of our knowledge, bioluminescence imaging is the only method available that allows analysis of ATP, glucose, and lactate concentrations at the necessary spatial resolution. 16 We have recently adapted this method to measure local ATP concentrations within the arterial wall. 14 In the present study, our methodology is extended to include determinations of glucose, glycogen, and lactate. The use of bioluminescence imaging to measure glycogen concentrations is presented...

show abstract

Novel concepts in atherogenesis: angiogenesis and hypoxia in atherosclerosis

Sluimer

Daemen

2009

The Journal of Pathology

324

222

View full text Add to dashboard Cite

The clinical complications of atherosclerosis are caused by thrombus formation, which in turn results from rupture of an unstable atherosclerotic plaque. The formation of microvessels (angiogenesis) in an atherosclerotic plaque contributes to the development of plaques, increasing the risk of rupture. Microvessel content increases with human plaque progression and is likely stimulated by plaque hypoxia, reactive oxygen species and hypoxia-inducible factor (HIF) signalling. The presence of plaque hypoxia is primarily determined by plaque inflammation (increasing oxygen demand), while the contribution of plaque thickness (reducing oxygen supply) seems to be minor. Inflammation and hypoxia are almost interchangeable and both stimuli may initiate HIF-driven angiogenesis in atherosclerosis. Despite the scarcity of microvessels in animal models, atherogenesis is not limited in these models. This suggests that abundant plaque angiogenesis is not a requirement for atherogenesis and may be a physiological response to the pathophysiological state of the arterial wall. However, the destruction of the integrity of microvessel endothelium likely leads to intraplaque haemorrhage and plaques at increased risk for rupture. Although a causal relation between the compromised microvessel structure and atherogenesis or between angiogenic stimuli and plaque angiogenesis remains tentative, both plaque angiogenesis and plaque hypoxia represent novel targets for non-invasive imaging of plaques at risk for rupture, potentially permitting early diagnosis and/or risk prediction of patients with atherosclerosis in the near future.

show abstract

In vitro tissue oxygen tensions in the rabbit aortic arch

Cited by 47 publications

References 21 publications

In vivo oxygen transport in the normal rabbit femoral arterial wall.

In vivo oxygen transport in the normal rabbit femoral arterial wall.

Mapping of ATP, Glucose, Glycogen, and Lactate Concentrations Within the Arterial Wall

Novel concepts in atherogenesis: angiogenesis and hypoxia in atherosclerosis

Contact Info

Product

Resources

About