In vitro toxicity evaluation of low doses of pesticides in individual and mixed condition on human keratinocyte cell line

Abhishek, Amar; Ansari, Nasreen Ghazi; Shankhwar, Satya Narayan; Jain, Amita; Singh, Vishwajeet

doi:10.6026/97320630010716

Cited by 22 publications

(9 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results showed that both agents enhanced ROS production. This is in accordance with the nding that α-HCH increased the ROS generation in keratinocytes [42] and that propanil induced lipid oxidation in rat models [43]. Mitochondria are the main source of intracellular ROS, and the ΔΨ was closely related to ROS production [44,45].…”

Section: Discussionsupporting

confidence: 89%

Interaction Between Genetic Variants and Serum Levels of Organochlorine Pesticides Contributes to Parkinson’s Disease

Xu¹,

Yang²,

Qian³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: There is evidence that genetic and environmental factors contribute to the onset and progression of Parkinson’s disease (PD). Pesticides are a class of environmental toxins that are linked to increased risk of PD. However, few studies have investigated the interaction between specific pesticides and genetic variants related to PD in the Chinese population.Methods: In this cross-sectional study, 19 serum levels of pesticides were measured. In addition, we also analyzed the interaction between specific pesticides and candidate genetic variants for PD. Finally, we investigated the mechanistic basis for the association between pesticides and increased risk of PD.Results: Serum levels of organochlorine pesticides including α-hexachlorocyclohexane (α-HCH), β-HCH, γ-HCH, δ-HCH, propanil, heptachlor, dieldrin, hexachlorobenzene, p,p’-dichlorodiphenyltrichloroethane (p,p’-DDE) and o,p’-dichloro-diphenyl-trichloroethane (o,p’-DDT) were higher in PD patients than in controls. α-HCH and propanil levels were associated with increased PD risk. Serum levels of dieldrin were associated with Hamilton Depression Scale and Montreal Cognitive Assessment scores in PD patients. The interaction between rs11931074 in α-synuclein (SNCA) and α-HCH or β-HCH, respectively, as well as rs16940758 in the microtubule-associated protein tau (MAPT) gene and δ-HCH were related to increased risk of PD. In addition, α-HCH and propanil enhanced the production of reactive oxygen species and decreased of mitochondrial membrane potential. Propanil but not α-HCH induced the aggregation of α-synuclein.Conclusions: Elevated serum levels of α-HCH and propanil are associated with increased risk of PD. Serum levels of dieldrin were associated with depression and cognitive function in PD patients. The interaction between genetic variants and pesticides also increased the risk of PD. Effects of genetic variants and pesticides on the risk of PD should be studied in more detail with a larger sample size to further understand the mechanisms involved.

show abstract

Section: Discussionsupporting

confidence: 89%

Interaction Between Genetic Variants and Serum Levels of Organochlorine Pesticides Contributes to Parkinson’s Disease

Xu¹,

Yang²,

Qian³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…For examples, Caron-Beaudoin et al 6 reported the adverse effects of atrazine on human adrenocortical carcinoma (H295R) and primary umbilical vein endothelial (HUVEC) cells 6 . Abhishek et al 7 reported the toxicity of three pesticides (i.e., alpha-hexachlorocyclohexane, parathion methyl and carbofuran) on human keratinocyte cells 7 .…”

mentioning

confidence: 99%

Novel synthesized 2, 4-DAPG analogues: antifungal activity, mechanism and toxicology

Gong

Tan

Chen

et al. 2016

Sci Rep

View full text Add to dashboard Cite

2, 4-Diacetylphloroglucinol (2,4-DAPG), a natural phenolic compound, has been investigated in light of its biological activities against plant pathogens. To improve its potential application, fourteen 2,4-DAPG analogous were synthesized through the Friedel-Crafts reaction using acyl chlorides and phloroglucinol. Of the 2,4-DAPG derivatives, MP4 exhibited much higher antifungal activity against Penicillium digitatum and P. italicum, the major pathogenic fungi in citrus fruit, than 2, 4-DAPG in vitro, and significantly inhibited the development of decay in harvested mandarin (Citrus reticulata Blanco cv. Shatang.) fruit in vivo. It was found that MP4 resulted in the wrinkle of the hyphae in both fungi with serious folds and breakage. In addition, the expression of several cytochrome P450 (CYP) genes were also modified in both fungi by MP4, which might be associated with the disorder of cell membrane formation. Furthermore, the toxicology of MP4 by evaluating the cell proliferation effect on human normal lung epithelial (16HBE) and kidney 293 (HEK293) cells, was significantly lower than that of albesilate, a widely used fungicide in harvested citrus fruit. In summary, the synthesized MP4 has shown a great potential as a novel fungicide that might be useful for control of postharvest decay in citrus fruit.

show abstract

“…In another study, HaCat cells were used to assess the combined effect of low dose of a mixture of three pesticides (lpha-hexachlorocyclohexane, parathion methyl and carbofuran). Results revealed that mixed pesticides were more hazardous for human health, exerting a high toxicity with respect to individual ones [75]. Deleterious consequences have been observed also with other organophosphate pesticides (such as omethoate and methamidophos) inducing different levels of cyto and genotoxic effects in human cell lines [76].…”

Section: Toxicological Studymentioning

confidence: 98%

Sonication-Assisted Production of Fosetyl-Al Nanocrystals: Investigation of Human Toxicity and In Vitro Antibacterial Efficacy against Xylella fastidiosa

Baldassarre

Tatulli²,

Vergaro

et al. 2020

Nanomaterials

View full text Add to dashboard Cite

Recently, there is a growing demand in sustainable phytopathogens control research. Nanotechnology provides several tools such as new pesticides formulations, antibacterial nanomaterials and smart delivery systems. Metal nano-oxides and different biopolymers have been exploited in order to develop nanopesticides which can offer a targeted solution minimizing side effects on environment and human health. This work proposed a nanotechnological approach to obtain a new formulation of systemic fungicide fosetyl-Al employing ultrasonication assisted production of water dispersible nanocrystals. Moreover, chitosan was applicated as a coating agent aiming a synergistic antimicrobial effect between biopolymer and fungicide. Fosetyl-Al nanocrystals have been characterized by morphological and physical-chemical analysis. Nanotoxicological investigation was carried out on human keratinocytes cells through cells viability test and ultrastructural analysis. In vitro planktonic growth, biofilm production and agar dilution assays have been conducted on two Xylella fastidiosa subspecies. Fosetyl-Al nanocrystals resulted very stable over time and less toxic respect to conventional formulation. Finally, chitosan-based fosetyl-Al nanocrystals showed an interesting antibacterial activity against Xylella fastidiosa subsp. pauca and Xylella fastidiosa subsp. fastidiosa.

show abstract

In vitro toxicity evaluation of low doses of pesticides in individual and mixed condition on human keratinocyte cell line

Cited by 22 publications

References 13 publications

Interaction Between Genetic Variants and Serum Levels of Organochlorine Pesticides Contributes to Parkinson’s Disease

Interaction Between Genetic Variants and Serum Levels of Organochlorine Pesticides Contributes to Parkinson’s Disease

Novel synthesized 2, 4-DAPG analogues: antifungal activity, mechanism and toxicology

Sonication-Assisted Production of Fosetyl-Al Nanocrystals: Investigation of Human Toxicity and In Vitro Antibacterial Efficacy against Xylella fastidiosa

Contact Info

Product

Resources

About