In vitro transcription assay of ozonated T7 phage dna

Mura, Cameron; Chung, Young Sup

doi:10.1002/em.2850160108

Cited by 9 publications

(5 citation statements)

References 20 publications

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“…Ozone can effectively kill bacteria and has the advantage that contact times for antimicrobial action are typically 4-5 times shorter than for chlorine (Zuma et al 2009a;Zuma and Jonnalagadda 2010). Previous investigations have found that ozone treatment can damage the cell membrane (Scott and Lesher 1963;Murray et al 1965;Kim et al 1999;Guzel-Seydim et al 2004;Cho et al 2010), facilitate protein denaturation and also destroy enzyme activity (Hinze et al 1987;Mehlman and Borek 1987;Takamoto et al 1992;Komanapalli and Lau 1996) and the nucleic acids of microbes (Scott and Lesher 1963;Roy et al 1981;Mura and Chung 1990;Hunt and Mariñas 1999). Other studies have investigated the effect of ozone on the spore inner membrane of B. subtilis (Cortezzo et al 2004;Young and Setlow 2004) and spore morphologies of Bacillus atrophaeus and Geobacillus stearothermophilus (Mahfoudh et al 2010).…”

Section: Discussionmentioning

confidence: 99%

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Effects of ozone on membrane permeability and ultrastructure in Pseudomonas aeruginosa

Zhang¹,

Wu²,

Zhang³

et al. 2011

Journal of Applied Microbiology

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Aims: To examine the mechanism of ozone‐induced damage to cytoplasmic membrane and cell ultrastructure of Pseudomonas aeruginosa ATCC27853. Methods and Results: Cell suspensions of Ps. aeruginosa ATCC27853 were treated with ozonated water. The leakages of cellular potassium (K+), magnesium (Mg2+) and adenosine triphosphate (ATP), determined by inductively coupled plasma/mass spectrometry (ICP/MS) and a commercial bioluminescence assay kit, were to assess ozone‐induced damage to the cytoplasmic membrane. Maximum leakages of K+ and Mg2+ were attained, respectively, at 0·53 mg l−1 ozone after 0·5 and 2 min with >99% inactivation of culturable bacteria, while that of ATP was achieved at 0·67 mg l−1 ozone after 1 min. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) revealed that treated cells retained intact shapes and cytoplasm agglutinations and vacuoles occurred. Conclusions: Ozone inactivates Ps. aeruginosa ATCC27853 by the combined results of increased cytoplasmic membrane permeability and cytoplasm coagulation, rather than by severe membrane disruption and cell lysis. Significance and Impact of the Study: Pseudomonas aeruginosa is a common water‐related pathogen. These insights into the leakage of cytoplasmic components and ultrastructural changes provide evidence for the mechanisms of ozone‐mediated inactivation.

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Section: Discussionmentioning

confidence: 99%

“…1992; Komanapalli and Lau 1996) and the nucleic acids of microbes (Scott and Lesher 1963; Roy et al. 1981; Mura and Chung 1990; Hunt and Mariñas 1999). Other studies have investigated the effect of ozone on the spore inner membrane of B. subtilis (Cortezzo et al.…”

Section: Discussionmentioning

confidence: 99%

Effects of ozone on membrane permeability and ultrastructure in Pseudomonas aeruginosa

Zhang¹,

Wu²,

Zhang³

et al. 2011

Journal of Applied Microbiology

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“…Ozone modified nucleic acids in vitro , with thymine being more sensitive than cytosine and uracil (Scott 1975;Ishizaki and others 1981). In another study, ozone opened the circular plasmid DNA and reduced its transforming ability, produced single-and double-strand breaks in plasmid DNA (Hamelin 1985), and decreased transcription activity (Mura and Chung 1990). Studying E. coli, l' Herault and Chung (1984) found that ozone may induce mutations.…”

Section: Mechanism Of Microbicidal Action Of Ozonementioning

confidence: 99%

Microbiological Aspects of Ozone Applications in Food: A Review

Khadre¹,

Yousef²,

Kim³

2001

Journal of Food Science

685

429

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Ozone is a powerful antimicrobial agent that is suitable for application in food in the gaseous and aqueous states. Molecular ozone or its decomposition products (for example, hydroxyl radical) inactivate microorganisms rapidly by reacting with intracellular enzymes, nucleic material and components of their cell envelope, spore coats, or viral capsids. Combination of ozone with appropriate initiators (for example, UV or H 2 O 2 ) results in advanced oxidation processes (AOPs) that are potentially effective against the most resistant microorganisms; however, applications of AOPs in food are yet to be developed. When applied to food, ozone is generated on-site and it decomposes quickly, leaving no residues. Ozone is suitable for decontaminating produce, equipment, foodcontact surfaces, and processing environment.

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“…Studies have been undertaken to elucidate the action mechanism of ozone. Researchers have found that it can damage cell membranes (Scott & Lesher, 1963;Murray et al, 1965;Kim et al, 1999;GuzelSeydim et al, 2004;Cho et al, 2010), facilitate protein denaturation and enzyme activity decrease (Hinze et al, 1987;Mehlman et al, 1987;Takamoto et al, 1992;Komanapalli & Lau, 1996), as well as destroy the nucleic acids of microbes (Scott & Lesher, 1963;Roy et al, 1981;Mura & Chung, 1990; Hunt & Mariñas, 1999). Other studies have investigated the effect of ozone on the spore inner membrane of Bacillus subtilis (Young & Setlow, 2004;Cortezzo et al, 2004), and on the spore morphologies of Bacillus atrophaeus as well as Geobacillus stearothermophilus (Mahfoudh et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Alteration in Escherichia coli and Streptococcus faecalis cells induced by ozone

2017

SDRP-JFST

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ABSTRACT:To elucidate ozone action mechanism, cell suspensions of E. coli 8099 and S. faecalis ATCC29212 were initially exposed to ozone for 5 min, and then treated with sodium dodecyl sulfate, sodium hydroxide, and proteinase K. those of S. faecalis were 7.95±2.81%-55.85±1.43%. After ozone pre-treatment, OD600 value of E. coli cells, sequently treated with SDS, NaOH, and PK, decreased by 8.83±1.86%, 34.72±0.42%, and 10.42±2.08%, respectively. And that of S. feacalis decreased by 2.85±3.63%, 71.30±1.08%, and 7.00±2.47%, respectively. Therefore, ozone pre-treatment made E. coli cells be sensitive to SDS and NaOH, not susceptible to PK. It was confirmed by transmission electron microscopy of E. coli cells. To S. faecalis, ozone pre-treatment activated the effects of SDS, NaOH, and PK on cells. ATP loss from S. faecalis cells indicated that ozone damaged cell membrane and increased membrane permeability. Because SDS-, NaOH-, and PK-induced cell lyses were respectively due to protein denaturation, cell membrane damage, and the reactions between PK and proteins or conjugated proteins, it was concluded that protein changes and cell membrane damage generated by ozone are responsible for E. coli and S. faecalis inactivation. Results showed that, after ozone treatment, OD600 reduction rates of E. coli cells were 6.11±1.29%-7.06±1.23%.

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In vitro transcription assay of ozonated T7 phage dna

Abstract: Address reprint request to Y.S. Chung, Universite de Montreal, DCpartement de Sciences Biologiques, CP 6128 Succ. A. Montreal (Que) H3C 3J7, Canada.

Cited by 9 publications

References 20 publications

Effects of ozone on membrane permeability and ultrastructure in Pseudomonas aeruginosa

Effects of ozone on membrane permeability and ultrastructure in Pseudomonas aeruginosa

Microbiological Aspects of Ozone Applications in Food: A Review

Alteration in Escherichia coli and Streptococcus faecalis cells induced by ozone

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