In-vitro- und In-vivo-Knochenregenerierung durch mesenchymale Stammzellen aus dem Nabelschnurblut

Jäger, Markus; Sager, Martin; Knipper, A.; Degistirici, Özer; Fischer, Johannes C.; Kögler, Gesine; Wernet, Peter; Krauspe, Rüdiger

doi:10.1007/s00132-004-0737-x

Cited by 21 publications

(3 citation statements)

References 37 publications

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“…In a study involving athymic rats, human UCB-MSCs displayed osteogenic differentiation in vitro , survived after xenotransplantation and differentiated into osteoblasts that filled the bony defects [10]. A recent study reported that human UCB-MSCs have a significantly stronger osteogenic potential but less capacity in adipogenic differentiation than bone marrow MSCs in vitro [4].…”

Section: Discussionmentioning

confidence: 99%

Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs

et al. 2008

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This study was performed to evaluate the osteogenic effect of allogenic canine umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) mixed with beta-tricalcium phosphate (β-TCP) in orthotopic implantation. Seven hundred milligrams of β-TCP mixed with 1 × 106 UCB-MSCs diluted with 0.5 ml of saline (group CM) and mixed with the same volume of saline as control (group C) were implanted into a 1.5 cm diaphyseal defect and wrapped with PLGC membrane in the radius of Beagle dogs. Radiographs of the antebrachium were made after surgery. The implants were harvested 12 weeks after implantation and specimens were stained with H&E, toluidine blue and Villanueva-Goldner stains for histological examination and histomorphometric analysis of new bone formation. Additionally, UCB-MSCs were applied to a dog with non-union fracture. Radiographically, continuity between implant and host bone was evident at only one of six interfaces in group C by 12 weeks, but in three of six interfaces in group CM. Radiolucency was found only near the bone end in group C at 12 weeks after implantation, but in the entire graft in group CM. Histologically, bone formation was observed around β-TCP in longitudinal sections of implant in both groups. Histomorphometric analysis revealed significantly increased new bone formation in group CM at 12 weeks after implantation (p < 0.05). When applied to the non-union fracture, fracture healing was identified by 6 weeks after injection of UCB-MSCs. The present study indicates that a mixture of UCB-MSCs and β-TCP is a promising osteogenic material for repairing bone defects.

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Section: Discussionmentioning

confidence: 99%

Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs

et al. 2008

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“…MC3T3 osteoblastic cells were seeded on cell culture plastic as a reference an on the different materials at a cell density of 10.000 cells /cm 2 in alpha‐minimum essential medium with 10% FBS and 1% penicillin–streptomycin and cultivated for 8 days in 48‐well cell culture plates in a cell culture incubator at standard cell culture conditions (37°C; 100% humidity, and 5% CO 2 ). Cell activity was measured at the start of the culture (day 0) and on day 8 using an 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium‐bromide (MTT)–enzyme‐linked immunoabsorbent assay cell culture test (Sigma–Aldrich) 26, 27. This in vitro test is based on the reduction of the yellow, water soluble tetrazolium salt to the blue‐violet colored formazan by metabolically active cells.…”

Section: Methodsmentioning

confidence: 99%

Influence of processing parameters on microstructure and biocompatibility of surface laser sintered hydroxyapatite–SiO₂ composites

Kivitz

Görke

Schilling³

et al. 2012

J Biomed Mater Res

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Silica-doped hydroxyapatite (HA) is a promising material concerning biocompatibility to natural bone, bioactivity and osteoconductive characteristics. HA exhibits phase transformations during sintering which are attendant to the change in volume and thermal strain. To avoid cracks during sintering, the exact knowledge of the phase transition temperatures is necessary. The sintering behavior of HA can be improved by adding amorphous silica with a low coefficient of thermal expansion. Therefore, the phase transformations in the system HA-SiO2 were analyzed by using differential scanning calorimetry followed by quantitative phase analysis by X-ray diffraction with the Riedveld method. The maximum sintering temperature without reversible phase transformation was defined as 1265°C. In laser surface sintered (LSS) samples, amorphous SiO2 , HA, and Si-α-TCP (or α-TCP) were detected. By comparison, only crystalline phases, such as cristobalite, HA, β-TCP, and Si-α-TCP (or α-TCP), were determined after furnace sintering. Scanning electron microscopy micrographs of furnace sintered and LSS samples show the differences in the resulting microstructures. Biocompatibility was determined by measuring cell activity of osteoblasts cultivated on four laser-sintered materials in the HA-SiO2 system in comparison to normal cell culture plastic. Cell proliferation was similar on all surfaces. The level of the cell activity on day 8 varied depending on the composition of the material and increased linearly as the amorphous SiO2 content rose. Taken together a laser-based method to develop novel biocompatible HA-SiO2 ceramics with adjustable properties and possible applications as orthopedic bioceramics are discussed.

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“…mesenchymaler Progenitorzellen (MPC) aus dem Nabelschnurblut intensiv wissenschaftlich erforscht [2,5,9]. So stellt nach Eblenkamp et al der Einsatz mesenchymaler Progenitorzellen zum gegenwärti-gen Zeitpunkt eines der spannendsten Felder der biomedizinischen Forschung dar [5].…”

Section: Introductionunclassified

Gewinnung mesenchymaler Progenitorzellen aus der humanen Nabelschnur

Lechner

Höcht²,

Ulrichs³

et al. 2007

Zentralbl Chir

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In-vitro- und In-vivo-Knochenregenerierung durch mesenchymale Stammzellen aus dem Nabelschnurblut

Abstract: Human USSC are a mesenchymal multipotent stem cell population that shows osteoblastic differentiation onto a collagen I/III carrier in vitro as well as in an athymic rat in vivo.

Cited by 21 publications

References 37 publications

Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs

Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs

Influence of processing parameters on microstructure and biocompatibility of surface laser sintered hydroxyapatite–SiO₂ composites

Gewinnung mesenchymaler Progenitorzellen aus der humanen Nabelschnur

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In-vitro- und In-vivo-Knochenregenerierung durch mesenchymale Stammzellen aus dem Nabelschnurblut

Abstract: Human USSC are a mesenchymal multipotent stem cell population that shows osteoblastic differentiation onto a collagen I/III carrier in vitro as well as in an athymic rat in vivo.

Cited by 21 publications

References 37 publications

Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs

Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs

Influence of processing parameters on microstructure and biocompatibility of surface laser sintered hydroxyapatite–SiO2 composites

Gewinnung mesenchymaler Progenitorzellen aus der humanen Nabelschnur

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Influence of processing parameters on microstructure and biocompatibility of surface laser sintered hydroxyapatite–SiO₂ composites