In vitro wound healing and cytotoxic activity of the gel and whole-leaf materials from selected aloe species

Fox, Lizelle T.; Mazumder, Anisha; Dwivedi, Anupma; Gerber, Minja; Plessis, Jeanetta du; Hamman, Josias H.

doi:10.1016/j.jep.2017.02.017

Cited by 82 publications

(54 citation statements)

References 20 publications

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“…The speed at which cells migrate toward the wounded area is known as migration rate and it indicates how fast the wound‐healing agent, PBM in our case, prompts the wound area to fill. We observed a significant increase in migration rate 24 hours after PBM2, PBM3 and PBM4 compared to NT cells (migration rate [mm 2 /h]: PBM2: 0.02 ± 0.005, P < 0.5; PBM3: 0.04 ± 0.004, P < 0.001; PBM4: 0.03 ± 0.004, P < 0.01; NT: 0.02 ± 0.005) (Figure C). Instead, 48 and 72 hours after PBM, we observed a significant increment in migration rate employing PBM3 and PBM4 protocols (migration rate [mm 2 /h]: PBM3 48 hours: 0.02 ± 0.003, P < 0.05; PBM4 48 hours: 0.02 ± 0.003, P < 0.05; NT 48 hours: 0.01 ± 0.001; PBM3 72 hours: 0.02 ± 0.002, P < 0.05; PBM4 72 hours: 0.02 ± 0.001, P < 0.05; NT 72 hours: 0.01 ± 0.003) (Figure C).…”

Section: Resultsmentioning

confidence: 99%

Photobiomodulation therapy promotes in vitro wound healing in nicastrin KO HaCaT cells

Tricarico

Zupin

Ottaviani

et al. 2018

Journal of Biophotonics

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Mutations in NCSTN gene (encoding for nicastrin protein) are associated with hidradenitis suppurativa (HS), a chronic inflammatory disease involving hair follicles. HS is clinically handled with drugs but the most severe cases are treated with surgery. Photobiomodulation (PBM) therapy, already used in the treatment of skin diseases such as acne, herpes virus lesions, ultraviolet damage, vitiligo, hypertrophic scar, keloid, burn, psoriasis and diabetic chronic wounds, could be beneficial as an adjuvant supportive treatment to promote and foster the healing process after skin excision in HS. The effects of PBM therapy in promoting the wound closure are evaluated in a HaCaT cells NCSTN-/-, assessing cell metabolism, migration rate, proliferation and cell cycle progression. In our experimental model, PBM exerts a potent action on metabolism of mutated keratinocytes, incrementing adenosine triphosphate (ATP) production at 2 hours, while after 24 hours an increase of metabolism with a decrement of intracellular ATP levels were recorded. Moreover, PBM speeds up the wound closure, inducing cells' migration without affecting their proliferation.Based on our findings, we suggest the use of PBM in HS patients, who undergo major surgery with large skin excision.

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Section: Resultsmentioning

confidence: 99%

Photobiomodulation therapy promotes in vitro wound healing in nicastrin KO HaCaT cells

Tricarico

Zupin

Ottaviani

et al. 2018

Journal of Biophotonics

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“…Wounds are described as physical or thermal injuries that result in a breaking or opening of the skin surface which causes a disruption of the normal skin anatomy and function [1,2]. In routine life, because of accidents and fires millions of people suffer from wounds, burns and cuts, therefore wounds, especially non-healing wounds, have became serious health care problem worldwide [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…These steps can be characterized into four highly programmed phases such as hemostasis, inflammation, proliferation and remodeling [2,6,[12][13][14][15][16]. All phases of healing must occur in suitable sequence for successful healing as a natural phenomenon [6,12,13] but sometimes complex multifactorial process of wound healing can be delayed or failed by unwanted effects such as presence of free radicals [12,17] or microbial infections [5,12,16,17].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Wound Healing Properties of Luteolin Ointments on Excision and Incision Wound Models in Diabetic and Non-Diabetic Rats

Özay¹,

Güzel²,

Erdoğdu³

et al. 2018

Rec.Nat.Prod.

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Luteolin, which is structurally classified under the flavonoids, is naturally distributed abundant among medicinal plants, vegetables and fruits. The wound healing effects of Luteolin ointments were evaluated for incision and excision wound models on non-diabetic and Streptozotocin induced diabetic rats by using macroscopical, biomechanical, biochemical and histopathological methods. Two different concentrations (0.5 % and 1 % (w/w)) of the Luteolin ointment were prepared with the mixture of glycol stearate:propylene glycol:liquid paraffin (3:6:1) and 0.5 g of the ointments were applied topically on wounds once daily for 7 and 14 days. During the experiments, wounds were visually observed, photographically documented and wound areas were measured. After 7 and 14 days treatment, animals were sacrificed under anesthesia and hydroxyproline measurement and biomechanical analysis were performed. Histopathology of the wound area was evaluated considering features of re-epithelialization, thickness of the granulation tissue, angiogenesis, presence of inflammation, number of mast cells. Outcomes of this study revealed that Luteolin ointments improved wound healing process of skin tissue both in non-diabetic and diabetic wounds. The best wound healing activity was observed in incision and excision wounds (97.6 %, 96.1 %, respectively) treated with 0.5 % (w/w) Luteolin ointment on day 14 according to macroscopic results.

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“…Aloe vera (Aloe barbadensis Miller) is the most widely used species, both commercially and for its therapeutic properties (Cardarelli et al, 2017;Kojo and Qian, 2004;Chen et al, 2012). This plant contains two materials with a juicy consistency: the first, a yellow exudate containing a high concentration of anthraquinone-type compounds, which have been used for decades as cathartics and purgatives, and the second, a clear mucilaginous gel that has been used since ancient times for the treatment of burns and other wounds (Fox et al, 2017;Reynolds and Dweck, 1999). Among the chemical components of the exudate are aloin, emodin, aloe-emodin, barbaloin, isobarbaloin and chrysophanic acid.…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of anthraquinones from Aloe vera (Aloe barbadensis Miller) roots and several derivatives against strains of influenza virus

Borges-Argáez

Chan-Balan

Cetina-Montejo

et al. 2019

Industrial Crops and Products

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In vitro wound healing and cytotoxic activity of the gel and whole-leaf materials from selected aloe species

Cited by 82 publications

References 20 publications

Photobiomodulation therapy promotes in vitro wound healing in nicastrin KO HaCaT cells

Photobiomodulation therapy promotes in vitro wound healing in nicastrin KO HaCaT cells

Evaluation of the Wound Healing Properties of Luteolin Ointments on Excision and Incision Wound Models in Diabetic and Non-Diabetic Rats

In vitro evaluation of anthraquinones from Aloe vera (Aloe barbadensis Miller) roots and several derivatives against strains of influenza virus

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