In vitro wound healing of tumor cells: inhibition of cell migration by selected cytotoxic alkaloids

Wang, Xiaojuan; Decker, Charlotte C.; Zechner, Laura; Krstin, Sonja; Wink, Michael

doi:10.1186/s40360-018-0284-4

Cited by 97 publications

(60 citation statements)

References 48 publications

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“…Wound healing assay on cancer cell was carried out according to previously reported method 26 . A549 lung cancer cells were cultured at a density of 1.5 × 10 6 cells/well for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

RETRACTED: Naringenin inhibits migration, invasion, induces apoptosis in human lung cancer cells and arrests tumour progression in vitro

Shi¹,

Luo²,

Chen³

et al. 2021

J Cellular Molecular Medi

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Lung cancer is one of the major cause for high‐death rate all over the world, due to increased metastasize and difficulties in diagnosis. Naringenin is naturally occurring flavonoid found in various fruits including tomatoes, citrus fruit and figs. Naringenin is known to have several therapeutic effects including anti‐atherogenic, antimicrobial, anti‐inflammatory, hepatoprotective, anticancer and anti‐mutagenic. The present study was aimed to analyse the naringenin induced anti‐proliferative and apoptosis effects in human lung cancer cells. Cells were treated with various concentrations of naringenin (10, 100 & 200 µmol/L) for 48 hours. Cisplatin (20 µg/mL) was used as positive control. Cell viability, apoptosis, migration and mRNA, and protein expression of caspase‐3, matrixmetallo proteinases‐2 (MMP‐2) and MMP‐9 were determined. The cell viability was 93.7 ± 7.5, 51.4 ± 4.4 and 32.1 ± 2.1 at 10, 100 and 200 µmol/L of naringenin respectively. Naringenin significantly increased apoptotic cells. The 100 and 200 µmol/L of naringenin significantly suppressed the larger wounds of cultured human cancer cells compared with the untreated lung cancer cells. Naringenin increased d the expression of caspase‐3 and reduced the expression of MMP‐2 and MMP‐9. Taking all these data together, it is suggested that the naringenin was effective against human lung cancer proliferation, migration and metastasis.

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“…Wound healing assay on cancer cell was carried out according to previously reported method 26 . A549 lung cancer cells were cultured at a density of 1.5 × 10 6 cells/well for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

RETRACTED: Naringenin inhibits migration, invasion, induces apoptosis in human lung cancer cells and arrests tumour progression in vitro

Shi¹,

Luo²,

Chen³

et al. 2021

J Cellular Molecular Medi

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show abstract

“…Therefore, the potential of 1 to suppress migration of MDA-MB-231 cancer cells indicates that this compound may prove as an efficient molecule in inhibiting the metastasis of cancer cells in vitro . 26 To clarify the role of 1 in cancer cell apoptosis and chemoresistance, we examined cell viability and apoptosis rate using 4′,6-diamidino-2-phenylindole (DAPI) staining and cell cytotoxicity assay [sulforhodamine B (SRB)]. 27 Our data demonstrated that 1 overexpression was able to inhibit cell viability, which was downregulated by 1 treatment.…”

Section: Resultsmentioning

confidence: 99%

Discovery of a Secalonic Acid Derivative fromAspergillus aculeatus, an Endophyte ofRosa damascenaMill., Triggers Apoptosis in MDA-MB-231 Triple Negative Breast Cancer Cells

et al. 2020

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A new secalonic acid derivative, F-7 ( 1 ), was isolated from the endophytic Aspergillus aculeatus MBT 102, associated with Rosa damascena . The planar structure of 1 was established on the basis of 1D and 2D NMR and ESI-TOF-MS spectra. The relative configuration of 1 was determined applying a combined quantum mechanical/NMR approach and, afterward, the comparison of calculated and experimental electronic circular dichroism spectra determined the assignment of its absolute configuration. The compound possesses strong cytotoxic activity against triple negative breast cancer (TNBC) cells. It was found to induce apoptosis, as evidenced by scanning electron microscopy and phase contrast microscopy. Furthermore, flow cytometry analyses demonstrated that 1 induced mitochondrial damage and reactive oxygen species mediated apoptosis, arresting the G1 phase of the cells in a dose-dependent manner. Also, the compound causes significant microtubule disruption in TNBC cells. Subsequently, 1 restricted the cell migration leading to the concomitant increase in expression of cleaved caspase and PARP.

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“…The wound healing and migration experiment offer the opportunity to observe cell migration, which plays an important role in several processes in tumor development, such as neoangiogenesis and metastasis. In cell culture as well as in vivo, microtubules are required for cell migration [ 38 ]. In this regard, it is important to determine the efficacy of microtubule-stabilizing agents such as PCX in wound-healing assays.…”

Section: Resultsmentioning

confidence: 99%

Therapeutic Efficacy and Biodistribution of Paclitaxel-Bound Amphiphilic Cyclodextrin Nanoparticles: Analyses in 3D Tumor Culture and Tumor-Bearing Animals In Vivo

Varan

Öztürk

et al. 2021

Nanomaterials

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The uniqueness of paclitaxel’s antimitotic action mechanism has fueled research toward its application in more effective and safer cancer treatments. However, the low water solubility, recrystallization, and side effects hinder the clinical success of classic paclitaxel chemotherapy. The aim of this study was to evaluate the in vivo efficacy and biodistribution of paclitaxel encapsulated in injectable amphiphilic cyclodextrin nanoparticles of different surface charges. It was found that paclitaxel-loaded amphiphilic cyclodextrin nanoparticles showed an antitumoral effect earlier than the drug solution. Moreover, the blank nanoparticles reduced the tumor growth with a similar trend to the paclitaxel solution. At 24 h, the nanoparticles had not accumulated in the heart and lungs according to the biodistribution assessed by in vivo imaging. Therefore, our results indicated that the amphiphilic cyclodextrin nanoparticles are potentially devoid of cardiac toxicity, which limits the clinical use and commercialization of certain polymeric nanoparticles. In conclusion, the amphiphilic cyclodextrin nanoparticles with different surface charge increased the efficiency of paclitaxel in vitro and in vivo. Cyclodextrin nanoparticles could be a good candidate vehicle for intravenous paclitaxel delivery.

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In vitro wound healing of tumor cells: inhibition of cell migration by selected cytotoxic alkaloids

Cited by 97 publications

References 48 publications

RETRACTED: Naringenin inhibits migration, invasion, induces apoptosis in human lung cancer cells and arrests tumour progression in vitro

RETRACTED: Naringenin inhibits migration, invasion, induces apoptosis in human lung cancer cells and arrests tumour progression in vitro

Discovery of a Secalonic Acid Derivative fromAspergillus aculeatus, an Endophyte ofRosa damascenaMill., Triggers Apoptosis in MDA-MB-231 Triple Negative Breast Cancer Cells

Therapeutic Efficacy and Biodistribution of Paclitaxel-Bound Amphiphilic Cyclodextrin Nanoparticles: Analyses in 3D Tumor Culture and Tumor-Bearing Animals In Vivo

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