In vitro α–amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit

Uddin, Nizam; Hasan, Md. Rakib; Hossain, Md. Monir; Sarker, Arjyabrata; Hasan, Ashraful; Islam, A. F. M. Mahmudul; Chowdhury, Mohd. Motaher H.; Rana, Md. Sohel

doi:10.12980/apjtb.4.2014c1173

Cited by 92 publications

(63 citation statements)

References 29 publications

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“…In a previous study Uddin et al (2014) performed the acute toxicity of this fruit extract. Administration of doses up to 4000 mg/kg (the highest dose) produced no mortality which was accompanied by normal physical activity of the tested animals [18]. This study strengthens the safety of the extract.…”

Section: Discussionsupporting

confidence: 74%

Assessment of Toxic Effects of the Methanol Extract of Citrus macroptera Montr. Fruit via Biochemical and Hematological Evaluation in Female Sprague-Dawley Rats

Uddin

Hasan

et al. 2014

PLoS ONE

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Citrus macroptera Montr. (C. macroptera) is locally known as Satkara. The fruit of this plant is used as appetite stimulant and in the treatment of fever. This study therefore aimed to evaluate the toxic effects of the fruit extract using some biochemical and hematological parameters in rat model. The effects of methanol extract of Citrus macroptera Montr. fruit administered at 250, 500 and 1000 mg/kg body weight were investigated on hematological and biochemical parameters in Sprague-Dawley female rats. Moreover, histopathological study was performed to observe the presence of pathological lesions in primary body organs. The extract presented no significant effect on body weight, percent water content, relative organ weight and hematological parameters in rat. Significant decrease from control group was observed in the levels of triglyceride, total cholesterol, low density lipoprotein and very low density lipoprotein; thus leading to significant decrease of cardiac risk ratio, castelli's risk index-2, atherogenic coefficient and atherogenic index of plasma at all doses. 500 mg/kg dose significantly decreased alkaline phosphatase (P<0.05), 1000 mg/kg dose significantly increased high density lipoprotein cholesterol (P<0.05) and 250 mg/kg dose significantly decreased the level of glycated hemoglobin (P<0.05) from the control group. There were no significant alterations observed with other serum biochemical parameters. Histopathological study confirmed the absence of inflammatory and necrotic features in the primary body organs. Study results indicate that methanolic fruit extract is unlikely to have significant toxicity. Moreover, these findings justified the cardio-protective, moderate hepato-protective and glucose controlling activities of the fruit extract.

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Section: Discussionsupporting

confidence: 74%

Assessment of Toxic Effects of the Methanol Extract of Citrus macroptera Montr. Fruit via Biochemical and Hematological Evaluation in Female Sprague-Dawley Rats

Uddin

Hasan

et al. 2014

PLoS ONE

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“…The hypoglycemiant properties of the raw extract and fractions of M. hirsuta was also assessed since the search for plants with antidiabetogenic potential has increased as an alternative to conventional treatment in as much as most commercial drugs have side effects (Uddin et al 2014, Rangika et al 2015. In addition, diabetes mellitus is one of the main issues for the high costs of public health services because of their worldwide incidence combined with high mortality and high morbidity indexes (Alarcon-Aguilar et al 2000).…”

Section: Phytochemistry and Toxicity Of Mansoa Hirsuta 323mentioning

confidence: 99%

Evaluation of cytogenotoxicity, antioxidant and hypoglycemiant activities of isolate compounds from Mansoa hirsuta D.C. (Bignoniaceae)

Pereira

Queiroz

Siqueira

et al. 2017

An. Acad. Bras. Ciênc.

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Mansoa hirsuta (Bignoniaceae) is a native plant from caatinga in Brazilian semiarid. This plant has been locally used as antimicrobial and hypoglycemiant agents, but their action mechanisms and toxicity remain largely unknown. Therefore, we evaluated the composition and antioxidant, cytoprotective and hypoglycemiant effects of raw extract, fractions and compounds from leaves of M. hirsuta. The cytogenotoxic effects of ursolic and oleanolic acids, the main phytotherapic components of this plant, were assessed. The raw extract and fractions presented steroids, saponins, flavonols, flavanonols, flavanones, xanthones, phenols, tannins, anthocyanins, anthocyanidins and flavonoids. The ethyl acetate fraction inhibited efficiently the cascade of lipid peroxidation while the hydroalcoholic fraction was richer in total phenols and more efficient in capturing 2,2-diphenyl-1-picrylhydrazyl ( · DPPH) and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS ·+ ) radicals. The isolated fraction of M. hirsuta also inhibited the α-amylase activity. Cytotoxic effects were absent in both raw extract and fractions while ursolic+oleanolic acids were efficient in protecting cells after exposure to hydrogen peroxide. Moreover, this mixture of acid shad no significant interference on the mitotic index and frequency of nuclear and/ or chromosomal abnormalities in Allium cepa test. Therefore, M. hirsuta represents a potential source of phytochemicals against inflammatory and oxidative pathologies, including diabetes.

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“…The peak is higher than that of the standard showing its potential in inhibiting the activity of an enzyme (Graph 1) and minimum inhibitory concentration (IC50) as 374(µg /ml). The alpha amylase inhibitory activity of the plant extract is due to the presence of secondary metabolites present in it (Uddin et al, 2014). Seed and leaf extract of S. cumini also reported to have a α-Glucosidase inhibitory activity and this activity is due to the presence of secondary metabolites apigenin 7-Oglucoside and dihydro-3,3',4',5,7 -pentahydroxyflavone glycoside respectively (Alagesan et al, 2012b).…”

Section: Resultsmentioning

confidence: 99%

In silico and in vitro assessment on antidiabetic efficacy of secondary metabolites from Syzygium cumini (L.) Skeels

Bansode¹,

Gupta

Salalkar³

2016

Plant Sci. Today

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India ranks high for prevalence of diabetes and the treatment of diabetes without any side effects is still challenging. Though herbal remedies help reduce the side effect, proper standardization of phytochemical which prove as a bioactive compound, its proper dose and clinical trials are lacking. In our investigation, we studied the binding mechanism of the secondary metabolites of Syzygium cumini, their in vitro antidiabetic activity and the number of phytochemicals present. In silico study revealed that ellagic acid has a potential to modulate the carbohydrate metabolizing enzyme activity showing higher affinity for the enzymes with much lesser binding energy, -4.73 kcal/mol for alpha amylase, -4.87 kcal/mol for beta-glucosidase, -4.79 kcal/mol for glycogen synthase kinase, -4.18 kcal/mol for glucokinase and -4.49 kcal/mol for alpha-glucosidase. In vitro-Alpha amylase inhibitory activity assay showed that ethanol extract has the highest value of percent inhibition (73.33%) as compared to standard drug Acarbose (65.99%). Finally, TLC analysis cleared that ethanol extract contains five compounds one of which may be a bioactive compound, ellagic acid. Further purification and characterization of the ellagic acid is needed.

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In vitro α–amylase inhibitory activity and in vivo hypoglycemic effect of methanol extract of Citrus macroptera Montr. fruit

Abstract: These findings suggest that the plant may be a potential source for the development of new oral hypoglycemic agent.

Cited by 92 publications

References 29 publications

Assessment of Toxic Effects of the Methanol Extract of Citrus macroptera Montr. Fruit via Biochemical and Hematological Evaluation in Female Sprague-Dawley Rats

Assessment of Toxic Effects of the Methanol Extract of Citrus macroptera Montr. Fruit via Biochemical and Hematological Evaluation in Female Sprague-Dawley Rats

Evaluation of cytogenotoxicity, antioxidant and hypoglycemiant activities of isolate compounds from Mansoa hirsuta D.C. (Bignoniaceae)

In silico and in vitro assessment on antidiabetic efficacy of secondary metabolites from Syzygium cumini (L.) Skeels

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