In vitroanticoccidial activity of essential oil microencapsulated against Eimeriaspp

Abstract

The aimof this research was to evaluate the in vitro anticoccidial activity of microencapsulated essential oils from Lepechinia heteromorpha(Briq.) Epling and Salvia sagittataRuiz & Pav., against non-attenuated sporulated oocysts of Eimeriasppof broiler chickens. The treatments were the concentrations of the microencapsulated in 2.5; 5; 10; 20;30 and 50 mg/ml, 2.5% potassium dichromate negative control group and 4% formaldehyde positive control group. The destruction of oocysts in liquid medium was evaluated by microscopic counting and the results were verified after 24 and 48 h of incubation at 28 °C. Data were analyzed by analysis of variance, using SPSS version 25 software and Tukey's test. The results showed highly significant statistical differences between the microencapsulated, as well as in their concentrations, registering the greatest destruction of sporulated oocysts by the microencapsulated essential oil of Salvia sagittataRuiz & Pav. at a concentration of 50 mg/ml at 48 h of incubation, reaching83.5% destruction of oocysts, this value being similar to 4% formaldehyde. Concluding that the microencapsulated essential oils studied destroy up to 83.5% of the sporulated oocysts of Eimeriaspp.