In vivo analgesic and anti-inflammatory effects of the essential oil from Tanacetum balsamita L.

Abstract

Tanacetum balsamita locally called Shahesparam is an aromatic plant that grows widely in Azerbaijan Province, Iran. Due to the widespread use of T. balsamita as a pain killer and relief of inflammatory based disorders in Iranian folk medicine and considering the high content of essential oil in T. balsamita aerial parts, we were prompted to investigate the anti-nociceptive and anti-inflammatory properties of T. balsamita essential oil (TBEO) for the first time. The carrageenan-induced Paw Edema was used for inflammation evaluation in rat, and hot-plate method was used for pain assessment in mice. Different doses of TBEO were administered. Morphine and Mefenamic acid were used as the standard drugs in antinociceptive and anti-inflammatory evaluation tests, respectively. TBEO (100 mg/kg) showed significantly anti-nociceptive activity in hot-plate test. The anti-inflammatory activity of TBEO was found to be more than mefenamic acid. The studied oil was analyzed by GC and GC-MS. The major component of the oil was characterized as carvone (39.8%) which might be responsible for the observed activities. The results suggested that TBEO possessed biologically active constituents that had significant analgesic and anti-inflammatory effects which support the ethno-medicinal claims of the plant application in the management of pain and inflammation.

Efficacy of Essential Oils in Pain: A Systematic Review and Meta-Analysis of Preclinical Evidence

“…Furthermore, in the study by Mishra et al (2010) Senecio rufinervis D.C. (Asteraceae) produced significant and dose-dependent inhibition of writhes and thermal hyperalgesia. In the study by Sharif et al (2020) Tanacetum balsamita (Compositae) presented an anti-hyperalgesic effect. The antinociceptive properties exerted by Xylopia laevigata (Annonaceae) in the acetic acid and in the formalin test have not proven dependency on opioid pathways ( Queiroz et al, 2014 ).…”

“…Attention to the circadian rhythm in behavioral testing was reported by the following studies: ( Andrade et al, 2012 ; Quintans et al, 2017 ; Guimaraes et al, 2009 ; Halder et al, 2012 ; Katsuyama et al, 2015 ; Khodabakhsh et al, 2015 ; Miraghazadeh et al, 2015 ; Sakurada et al, 2011 ; Sharif et al, 2020 ; Zarei et al, 2018 ; Zendehdel et al, 2015 ; Komatsu et al, 2018b ; Kuwahata et al, 2013 ). All the studies used control and positive and negative modulators.…”

“…A statement of compliance with animal welfare regulations is reported in all the studies since it is an inclusion criterion. Moreover, six studies ( Ulku Karabay–Yavasoglu et al, 2006 ; Venâncio et al, 2011 ; Queiroz et al, 2014 ; Khodabakhsh et al, 2015 ; Miraghazadeh et al, 2015 ; Sharif et al, 2020 ) also stated that they used each mouse only once, thus proving particular attention to animal welfare. Importantly, only the study by Hamamura et al (2020) reported acclimatization to lighting conditions for one week and that an observation period of 14 days can model examination during chronic pain.…”

“…Importantly, sham procedure and the certainty of exact execution of the pain model is present only in studies on allodynia, i.e., the studies by Hamamura et al (2020), Komatsu et al (2018a), and Kuwahata et al (2013). Attention to the circadian rhythm in behavioral testing was reported by the following studies: (Andrade et al, 2012;Quintans et al, 2017;Guimaraes et al, 2009;Halder et al, 2012;Katsuyama et al, 2015;Khodabakhsh et al, 2015;Miraghazadeh et al, 2015;Sakurada et al, 2011;Sharif et al, 2020;Zarei et al, 2018;Zendehdel et al, 2015;Komatsu et al, 2018b;Kuwahata et al, 2013). All the studies used control and positive and negative modulators.…”

The essential oil from Kelussia odoratissimaMozaff. fruits exerting potent analgesic and anti-inflammatory effects

“…Severe anti-inflammatory action was evaluated in terms of inhibition of the paw edema caused by injecting carrageenan (0.1 mL, 2%) into the subplantar area of the mice's right hind paw (Sharif et al, 2020). The male rats were assigned to 5 groups, along with five rats per group.…”