In vivo analytical techniques facilitated by contemporary materials

Godage, Nipunika H.; Olomukoro, Aghogho A.; Emmons, Ronald V.; Gionfriddo, Emanuela

doi:10.1016/j.trac.2021.116290

Cited by 6 publications

(2 citation statements)

References 81 publications

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“…The advantage of SPME as a sample preparation technique lies in its ability to selectively extract only the free concentration of the desired analytes, effectively isolating them from the complex sample mixture [92,93]. Furthermore, SPME offers the unique capability of extracting polar and non-polar analytes from biological matrices [91]. This versatility has been demonstrated by Jaroch et al, who explored the application of SPME as an extraction tool for in-vivo mouse melanoma models [98].…”

Section: 25mentioning

confidence: 99%

“…This study showcases the wide applicability of SPME in diverse biological systems. Consequently, SPME has gained increasing importance in metabolomics studies where living systems can be studied without disturbances [89,91]. The value of SPME as a sample preparation technique in analytical extractions cannot be overstated, particularly in mitigating MEs.…”

Section: 25mentioning

confidence: 99%

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Matrix effects demystified: Strategies for resolving challenges in analytical separations of complex samples

Williams,

Olomukoro,

Emmons

et al. 2023

J of Separation Science

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Matrix effects can significantly impede the accuracy, sensitivity, and reliability of separation techniques presenting a formidable challenge to the analytical process. It is crucial to address matrix effects to achieve accurate and precise measurements in complex matrices. The multifaceted nature of matrix effects which can be influenced by factors such as target analyte, sample preparation protocol, composition, and choice of instrument necessitates a pragmatic approach when analyzing complex matrices. This review aims to highlight common challenges associated with matrix effects throughout the entire analytical process with emphasis on gas chromatography‐mass spectrometry, liquid chromatography‐mass spectrometry, and sample preparation techniques. These techniques are susceptible to matrix effects that could lead to ion suppression/enhancement or impact the analyte signal at various stages of the analytical workflow. The assessment, quantification, and mitigation of matrix effects are necessary in developing any analytical method. Strategies can be implemented to reduce or eliminate the matrix effect by changing the type of ionization, improving extraction and clean‐up methods, optimization of chromatography conditions, and corrective calibration methods. While development of an effective strategy to completely mitigate matrix effects remains elusive, an integrated approach that combines sample preparation, analytical extraction, and effective instrumental analysis remains the most promising avenue for identifying and resolving matrix effects.

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