In Vivo and In Vitro Studies of Epithelial Cell Behavior around Titanium Implants with Machined and Rough Surfaces

Atsuta, Ikiru; Ayukawa, Yasunori; Furuhashi, Akihiro; Ogino, Yoichiro; Moriyama, Yasushi; Tsukiyama, Yoshihiro; Koyano, Kiyoshi

doi:10.1111/cid.12043

Cited by 39 publications

(50 citation statements)

References 32 publications

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“…First, we investigated the OECs adhesion to Ti and Zr plates with fluorescence staining. As shown in Figure A, numerous intracellular punctate signals of adhesion proteins were observed on both plates, supporting previous findings with rat OECs on Ti plates . However, we observed slight differences in Ln‐332, In‐β4, and plectin expression between the substrates at the point of accumulation.…”

Section: Discussionsupporting

confidence: 91%

“…As shown in Figure 5A, numerous intracellular punctate signals of adhesion proteins were observed on both plates, supporting previous findings with rat OECs on Ti plates. 16 However, we observed slight differences in Ln-332, In-β4, and plectin expression between the substrates at the point of accumulation. In the western blotting analyses, OECs on the Zr plates also had slightly weaker expression of In-β4 or Plectin than those on the Ti plates (Fig.…”

Section: Discussionmentioning

confidence: 59%

“…Pure Zr and Ti plates (99.9 mass%, Sky Blue) were used for these experiments, previously. 16 And OEC cultures were from the oral mucosa of 4-day-old Wistar as previous reports. 16,17 The cells were cultured in Defined Keratinocyte Serum Free Medium (DK-SFM; Thermo Fisher Scientific, Waltham, MA) on Ti or Zr plate.…”

Section: Culture Experimentsmentioning

confidence: 99%

“…Confluent monolayers of OECs on Zr and Ti plates were wounded with a cell scraper and cultured for 48 h, as previous article. 16 Cells at the edge of the wounds were observed by fluorescence using the phalloidin and anti-In-β4 antibodies.…”

Section: Scratch Assaysmentioning

confidence: 99%

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Epithelial sealing effectiveness against titanium or zirconia implants surface

Atsuta

Ayukawa

Furuhashi

et al. 2019

J Biomedical Materials Res

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The aims of implant treatment now involve not only restoration of mastication function, but also recovery of esthetics. Currently, zirconia is widely used as an esthetic material for implant abutment. Therefore, it is very important to understand the efficacy of zirconia for epithelial sealing as an implant material. We compared the effects of materials on the sealing of the peri‐implant epithelium (PIE) to titanium (Ti) or zirconia (Zr) implants, for application to clinical work. Maxillary first molars were extracted from rats and replaced with Ti or Zr implants. The sealing of the PIE to the implants was evaluated with immunohistochemistry observation and HRP analysis. The morphological and functional changes in rat oral epithelial cells (OECs) cultured on Ti or Zr plates were also evaluated. After 4 weeks, the PIE on the Ti and Zr implants showed similar structures. The Zr implants appeared to form a weak epithelial seal at the tissue–implant interface, and exhibited markedly less adhesive structures than the Ti implants under electron microscopic observation. In the in vitro experiments, decreased expression levels of adhesion proteins were observed in OECs cultured on Zr plates compared with those cultured on Ti plates. In addition, the cell adherence on Zr plates was reduced, while the cell migration was low on Ti plates. Zr is a better choice for an esthetic implant material, but needs further improvement for integration with the epithelial wound healing process around a dental implant. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 59%

Section: Culture Experimentsmentioning

confidence: 99%

Section: Scratch Assaysmentioning

confidence: 99%

See 2 more Smart Citations

Epithelial sealing effectiveness against titanium or zirconia implants surface

Atsuta

Ayukawa

Furuhashi

et al. 2019

J Biomedical Materials Res

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“…However, these alterations can also induce a change in the surface topography. Considering that a roughened surface has a negative effect on soft tissue cell adhesion [7,8], such alterations often lead to dental plaque accumulation on exposed implant surfaces in the oral cavity. A previous study showed that spontaneous progression of peri-implantitis was more pronounced around implants with a roughened surface compared with implants with a polished surface [9].…”

Section: Introductionmentioning

confidence: 99%

Effects of CaCl2 hydrothermal treatment of titanium implant surfaces on early epithelial sealing

Oshiro

Ayukawa

Atsuta

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Strategies for Optimizing the Soft Tissue Seal around Osseointegrated Implants

Abdallah

Badran

Ciobanu

et al. 2017

Adv Healthcare Materials

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Percutaneous and permucosal devices such as catheters, infusion pumps, orthopedic, and dental implants are commonly used in medical treatments. However, these useful devices breach the soft tissue barrier that protects the body from the outer environment, and thus increase bacterial infections resulting in morbidity and mortality. Such associated infections can be prevented if these devices are effectively integrated with the surrounding soft tissue, and thus creating a strong seal from the surrounding environment. However, so far, there are no percutaneous/permucosal medical devices able to prevent infection by achieving strong integration at the soft tissue-device interface. This review gives an insight into the current status of research into soft tissue-implant interface and the challenges associated with these interfaces. Biological soft/hard tissue interfaces may provide insights toward engineering better soft tissue interfaces around percutaneous devices. In this review, focus is put on the history and current findings as well as recent progress of the strategies aiming to develop a strong soft tissue seal around osseointegrated implants, such as orthopedic and dental implants.

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In Vivo and In Vitro Studies of Epithelial Cell Behavior around Titanium Implants with Machined and Rough Surfaces

Abstract: Ms implants are a better choice for integration with an epithelial wound healing process.

Cited by 39 publications

References 32 publications

Epithelial sealing effectiveness against titanium or zirconia implants surface

Epithelial sealing effectiveness against titanium or zirconia implants surface

Effects of CaCl2 hydrothermal treatment of titanium implant surfaces on early epithelial sealing

Strategies for Optimizing the Soft Tissue Seal around Osseointegrated Implants

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