In vivo and in vitro differentiation of myocytes from human bone marrow–derived multipotent progenitor cells

Muguruma, Yukari; Reyes, Morayma; Nakamura, Yoshihiko; Sato, Tadayuki; Matsuzawa, Hideyuki; Miyatake, Hiroko; Akatsuka, Akira; Itoh, Johbu; Yahata, Takashi; Ando, Kiyoshi; Kato, Shingo; Hotta, Tomomitsu

doi:10.1016/j.exphem.2003.09.003

Cited by 92 publications

(76 citation statements)

References 45 publications

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“…Human MAPCs and hMSCs did not differentiate toward skeletal muscle cells using the 2% HS protocol as assessed by RT-qPCR (not shown) or myotube formation (Fig. 2G), or when applying previously described protocols including addition of 5-azacytidine (10 lM) or vascular endothelial growth factor (VEGF), bFGF, and insulin-like growth factor 1 (IGF1) (not shown) [28,29].…”

Section: Resultsmentioning

confidence: 99%

Differentiation Potential of Human Postnatal Mesenchymal Stem Cells, Mesoangioblasts, and Multipotent Adult Progenitor Cells Reflected in Their Transcriptome and Partially Influenced by the Culture Conditions

et al. 2011

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Several adherent postnatal stem cells have been described with different phenotypic and functional properties. As many of these cells are being considered for clinical therapies, it is of great importance that the identity and potency of these products is validated. We compared the phenotype and functional characteristics of human mesenchymal stem cells (hMSCs), human mesoangioblasts (hMab), and human multipotent adult progenitor cells (hMAPCs) using uniform standardized methods. Human MAPCs could be expanded significantly longer in culture. Differences in cell surface marker expression were found among the three cell populations with CD140b being a distinctive marker among the three cell types. Differentiation capacity towards adipocytes, osteoblasts, chondrocytes, and smooth muscle cells in vitro, using established protocols, was similar among the three cell types. However, only hMab differentiated to skeletal myocytes, while only hMAPCs differentiated to endothelium in vitro and in vivo. A comparative transcriptome analysis confirmed that the three cell populations are distinct and revealed gene signatures that correlated with their specific functional properties. Furthermore, we assessed whether the phenotypic, functional, and transcriptome features were mediated by the culture conditions. Human MSCs and hMab cultured under MAPC conditions became capable of generating endothelial-like cells, whereas hMab lost some of their ability to generate myotubes. By contrast, hMAPCs cultured under MSC conditions lost their endothelial differentiation capacity, whereas this was retained when cultured under Mab conditions, however, myogenic capacity was not gained under Mab conditions. These studies demonstrate that hMSCs, hMab, and hMAPCs have different properties that are partially mediated by the culture conditions.

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Section: Resultsmentioning

confidence: 99%

Differentiation Potential of Human Postnatal Mesenchymal Stem Cells, Mesoangioblasts, and Multipotent Adult Progenitor Cells Reflected in Their Transcriptome and Partially Influenced by the Culture Conditions

et al. 2011

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“…40,41 Since BM contains heterogenous populations of TCSC, our data explain, without invoking the concept of HSC plasticity, why several investigators were able to demonstrate in vitro cultures the appearance of skeletal muscle, liver, pancreatic or neural cells growing from plated BM-, mPB-or CB-derived cells. 43,[63][64][65][66][67][68][69][70][71][72][73][74][75][76][77][78][79][80][81][82] Separation of BM-derived HSC from TCSC Since both HSC and TCSC express CXCR4, it was obvious that by employing chemotactic isolation to an SDF-1 gradient, we will isolate from BMMNC both CXCR4 þ HSC and nonhematopoietic stem cells. Thus to separate both of these populations of stem cells, we stained them with antibodies against panhematopoietic antigen CD45 and subsequently sorted CD45 þ and CD45 À cells by FACS (Figure 3).…”

Section: Bm Stem Cells Are Heterogeneousmentioning

confidence: 99%

Bone marrow as a home of heterogenous populations of nonhematopoietic stem cells

et al. 2005

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Evidence is presented that bone marrow (BM) in addition to CD45 positive hematopoietic stem cells contains a rare population of heterogenous CD45 negative nonhematopoietic tissue committed stem cells (TCSC). These nonhematopoietic TCSC (i) are enriched in population of CXCR4 þ CD34 þ AC133 þ lin À CD45 À and CXCR4 þ Sca-1 þ lin À CD45 À in humans and mice, respectively, (ii) display several markers of pluripotent stem cells (PSC) and (iii) as we envision are deposited in BM early in development. Thus, since BM contains versatile nonhematopoietic stem cells, previous studies on plasticity trans-dedifferentiation of BM-derived hematopoietic stem cells (HSC) that did not include proper controls to exclude this possibility could lead to wrong interpretations. Therefore, in this spotlight review we present this alternative explanation of 'plasticity' of BMderived stem cells based on the assumption that BM stem cells are heterogenous. We also discuss a potential relationship of TCSC/PSC identified by us with other BM-derived CD45 negative nonhematopoietic stem cells that were recently identified by other investigators (eg MSC, MAPC, USSC and MIAMI cells). Finally, we discuss perspectives and pitfalls in potential application of these cells in regenerative medicine.

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“…For instance, mesenchymal stem cells (MSC) can differentiate into blood, myogenic, vascular or neurogenic cells (Jiang et al, 2002;Muguruma et al, 2003;Kim et al, 2005); skin stem cells can differentiate into neurons, smooth muscle cells or adipose cells (Toma et al, 2001(Toma et al, , 2005 and adipose stem cells can create osteogenic, myogenic, or chondrogenic lineages (Zuk et al, 2002;Lin et al, 2005). These findings indicate that adult stem cells have the capability to differentiate into tissues other than the tissues from which they originated.…”

Section: Introductionmentioning

confidence: 94%

Isolation of neural precursor cells from skeletal muscle tissues and their differentiation into neuron-like cells

Park

Kim²,

Han³

et al. 2007

Exp Mol Med

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Skeletal muscle contains several precursor cells that generate muscle, bone, cartilage and blood cells. Although there are reports that skeletal muscle-derived cells can trans-differentiate into neural-lineage cells, methods for isolating precursor cells, and procedures for successful neural induction have not been fully established. Here, we show that the preplate cell isolation method, which separates cells based on their adhesion characteristics, permits separation of cells possessing neural precursor characteristics from other cells of skeletal muscle tissues. We term these isolated cells skeletal muscle-derived neural precursor cells (SMNPs). The isolated SMNPs constitutively expressed neural stem cell markers. In addition, we describe effective neural induction materials permitting the neuron-like cell differentiation of SMNPs. Treatment with retinoic acid or forskolin facilitated morphological changes in SMNPs; they differentiated into neuron-like cells that possessed specific neuronal markers. These results suggest that the preplate isolation method, and treatment with retinoic acid or forskolin, may provide vital assistance in the use of SMNPs in cell-based therapy of neuronal disease.

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In vivo and in vitro differentiation of myocytes from human bone marrow–derived multipotent progenitor cells

Cited by 92 publications

References 45 publications

Differentiation Potential of Human Postnatal Mesenchymal Stem Cells, Mesoangioblasts, and Multipotent Adult Progenitor Cells Reflected in Their Transcriptome and Partially Influenced by the Culture Conditions

Differentiation Potential of Human Postnatal Mesenchymal Stem Cells, Mesoangioblasts, and Multipotent Adult Progenitor Cells Reflected in Their Transcriptome and Partially Influenced by the Culture Conditions

Bone marrow as a home of heterogenous populations of nonhematopoietic stem cells

Isolation of neural precursor cells from skeletal muscle tissues and their differentiation into neuron-like cells

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