1992
DOI: 10.1182/blood.v80.12.3044.bloodjournal80123044
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In vivo and in vitro stem cell function of c-kit- and Sca-1-positive murine hematopoietic cells

Abstract: c-kit is expressed on hematopoietic stem cells and progenitor cells, but not on lymphohematopoietic differentiated cells. Lineage marker- negative, c-kit-positive (Lin-c-kit+) bone marrow cells were fractionated by means of Ly6A/E or Sca-1 expression. Lin-c-kit+Sca-1+ cells, which consisted of 0.08% of bone marrow nucleated cells, did not contain day-8 colony-forming units-spleen (CFU-S), but 80% were day-12 CFU-S. One hundred cells rescued the lethally irradiated mice and reconstituted hematopoiesis. On the o… Show more

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Cited by 79 publications
(79 citation statements)
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“…During differentiation, c-Kit expression progressively declines in most cell types, however, it remains very prominent through all stages of mast cell development including their progenitors and precursors in spleen and fetal blood [28,29]. In fact, c-Kit cell surface expression levels are 5.5-fold while its mRNA levels are almost 60-fold higher in mature peritoneal mast cells compared to hematopoietic stem cells that are present in the bone marrow lin − Sca-1 + c-Kit + (LSK) population [30] (Fig. 1A).…”
Section: Resultsmentioning
confidence: 99%
“…During differentiation, c-Kit expression progressively declines in most cell types, however, it remains very prominent through all stages of mast cell development including their progenitors and precursors in spleen and fetal blood [28,29]. In fact, c-Kit cell surface expression levels are 5.5-fold while its mRNA levels are almost 60-fold higher in mature peritoneal mast cells compared to hematopoietic stem cells that are present in the bone marrow lin − Sca-1 + c-Kit + (LSK) population [30] (Fig. 1A).…”
Section: Resultsmentioning
confidence: 99%
“…Commonly used markers include Thy1.1, c-Kit and Sca1. Thus, multipotent hematopoietic progenitors have been purified as Lin − Thy1.1 lo c-Kit + Sca-1 + cells that make up <0.1% of nucleated BM cells [897][898][899][900]. Although this population contains all multipotent progenitors in mice, it is still heterogeneous, containing transiently reconstituting multipotent progenitors in addition to long-term reconstituting HSCs.…”
Section: Introductionmentioning
confidence: 99%
“…23,24 Although the proportion of K + S + cells in the lineage-negative fraction (K + S + L − ) was maintained in G2 fGN/fGN mice ( Figure 3D), the cells were still heterogeneous and mixed with lineage-primed multipotent progenitors and short-term and longterm HSCs. 25 Therefore, we used the Hoechst-effluxing side population (SP) assay because long-term repopulating HSCs are known to be highly concentrated in the SP fraction. 26 We found that SP cells were decreased considerably in the K + S + L − cell population from the BM of G2 fGN/fGN mice ( Figure 3E).…”
Section: Hematopoietic Progenitors Are Increased In G2 Fgn/fgn Micementioning
confidence: 99%