In-vivo and in-vitro testing to assess the bioaccessibility and the bioavailability of arsenic, selenium and mercury species in food samples

Moreda–Piñeiro, Jorge; Moreda–Piñeiro, Antonio; Romarís–Hortas, Vanessa; Moscoso-Pérez, Carmen; López-Mahı́a, Purificación; Muniategui-Lorenzo, Soledad; Bermejo-Barrera, Pilar; Prada‐Rodríguez, D.

doi:10.1016/j.trac.2010.09.008

Cited by 155 publications

(75 citation statements)

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“…The in vitro extraction can be strongly affected by various conditions, such as temperature, pH, agitation and extract composition, which should be similar to those in the human body during digestion. Simulated body fluid (SBF) tests have been used to study the bioaccessibility of several metals in food (Juhasz et al, 2006;Moreda-Pineiro et al, 2011), soil (Kientz et al, 2003), and sediment (Semple et al, 2004), in which the bioaccessible fraction was extracted by using simulated gastric fluid (SGF) or SGF and simulated intestinal fluid (SIF) together. Cabanero et al (2004) have used the SBF test to study the Hg bioaccessibility in fishes.…”

Section: Introductionmentioning

confidence: 99%

Estimation of bioaccessibility and potential human health risk of mercury in Chinese patent medicines

Yun

Jiang

2016

Journal of Environmental Sciences

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Section: Introductionmentioning

confidence: 99%

Estimation of bioaccessibility and potential human health risk of mercury in Chinese patent medicines

Yun

Jiang

2016

Journal of Environmental Sciences

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“…Estimating element bioavailability in food has been performed by in vivo (by supplementing animals and humans with target compounds) and in vitro methods [13]. The latter is often performed by simulating gastrointestinal conditions as those found in the human body and analysing the dialyzable fraction of the compound, which can dialyze through a semipermeable membrane with a specified pore size (dialyzate or bioavailable fraction).…”

Section: Introductionmentioning

confidence: 99%

Two-dimensional HPLC coupled to ICP-MS and electrospray ionisation (ESI)-MS/MS for investigating the bioavailability in vitro of arsenic species from edible seaweed

García-Sartal

Taebunpakul

Stokes³

et al. 2011

Anal Bioanal Chem

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Edible seaweed consumption is a route of exposure to arsenic. However, little attention has been paid to estimate the bioaccessibility and/or bioavailability of arsenosugars in edible seaweed and their possible degradation products during gastrointestinal digestion. This work presents first use of combined inductively coupled plasma mass spectroscopy (ICP-MS) with electrospray ionization tandem mass spectrometry (ESI-MS/MS) with two-dimensional HPLC (size exclusion followed by anion exchange) to compare the qualitative and quantitative arsenosugars speciation of different edible seaweed with that of their bioavailable fraction as obtained using an in vitro gastrointestinal digestion procedure. Optimal extraction conditions for As species from four seaweed namely kombu, wakame, nori and sea lettuce were selected as a compromise between As extraction efficiency and preservation of compound identity. For most investigated samples, the use of ammonium acetate buffer as extractant and 1 h sonication in a water bath followed by HPLC-ICP-MS resulted in 40-61% of the total As to be found in the buffered aqueous extract, of which 86-110% was present as arsenosugars (glycerol sugar, phosphate sugar and sulfonate sugar for wakame and kombu and glycerol sugar and phosphate sugar for nori). The exception was sea lettuce, for which the arsenosugar fraction (glycerol sugar, phosphate sugar) only comprised 44% of the total extracted As. Interestingly, the ratio of arsenobetaine and dimethylarsinic acid to arsenosugars in sea lettuce extracts seemed higher than that for the rest of investigated samples. After in vitro gastrointestinal digestion, approximately 11-16% of the total As in the solid sample was found in the dialyzates with arsenosugars comprising 93-120% and 41% of the dialyzable As fraction for kombu, wakame, nori and sea lettuce, respectively. Moreover, the relative As species distribution in seaweed-buffered extracts and dialyzates was found to be very similar. Collection of specific fractions from the size-exclusion column to be analysed using anion-exchange HPLC-ESI-MS/MS enabled improved chromatographic selectivity, particularly for the less retained arsenosugar (glycerol sugar), facilitating confirmation of the presence of arsenosugars in seaweed extracts and dialyzates. Using this approach, the presence of arsenobetaine in sea lettuce samples was also confirmed.

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“…In place of such processing, use is normally made of a homogenization step, though this does not create a bolus. In the case of liquid foods or isolated food components, the homogenization and bolus formation phase is not performed, though salivary amylase may be added (Moreda-Piñeiro et al 2011 ). For example, in studies on the hydrolysis of proteins there is no significant enzyme action in the mouth (Wickham et al 2009 ), although an oral phase has been applied for other components (carotenoids, plant sterols and minerals).…”

Section: Digestion Conditionsmentioning

confidence: 99%

Static Digestion Models: General Introduction

Alegrı́a

García‐Llatas

Cilla

2015

The Impact of Food Bioactives on Health

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Several in vitro methods have been developed to simulate the physiological conditions of the human gastrointestinal digestion, the simplest being the static methods. The following chapter clarifi es the concepts of bioaccessibility and dialyzability, and describes the conditions (pH, enzymes, agitation, etc.) to be applied in oral, gastric and intestinal phases when assessing a food component (nutrient, bioactive or toxin) or a food product, in a single or multi-phase model. The advantages and disadvantages of the static models vs. dynamic and in vivo models are discussed, and a review of specifi c conditions applied on nutrients (minerals, vitamins, proteins, fatty acids, etc.) and bioactive compounds (carotenoids, plant sterols, etc.) from recent studies is provided. Currently, it must be considered that, although the static digestion conditions must be adapted according to the component or food sample to be studied, a harmonization and standardization of the models are needed in order to establish suitable correlations among in vitro and in vivo assays, as it has been defi ned for some food components (carotenoids, proteins and minerals).

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In-vivo and in-vitro testing to assess the bioaccessibility and the bioavailability of arsenic, selenium and mercury species in food samples

Cited by 155 publications

References 45 publications

Estimation of bioaccessibility and potential human health risk of mercury in Chinese patent medicines

Estimation of bioaccessibility and potential human health risk of mercury in Chinese patent medicines

Two-dimensional HPLC coupled to ICP-MS and electrospray ionisation (ESI)-MS/MS for investigating the bioavailability in vitro of arsenic species from edible seaweed

Static Digestion Models: General Introduction

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