In Vivo Anti-Inflammatory, Analgesic, Sedative, Muscle Relaxant Activities and Molecular Docking Analysis of Phytochemicals from Euphorbia pulcherrima

Abstract

Euphorbia pulcherrima is an important medicinal plant that is used in a traditional system for its curative properties such as analgesic potency, antipyretic, anti-inflammatory, sedation potential, and antidepressant and cure of diseases such as skin diseases. This study deals with the isolation of two flavonoids namely spinacetin (1) and patuletin (2) from chloroform fraction of Euphorbia pulcherrima. The isolated compound spinacetin (1) and patuletin (2) were screened for in vivo anti-inflammatory, analgesic, sedative, and muscle relaxant effects. Compounds 1 and 2 were assessed against hot plate-induced noxious stimuli at various doses which showed excellent ( p < 0.05 ) analgesic effect in a dose-dependent manner. The muscle relaxant activity was determined by traction and inclined screening model, both compounds showed significant muscle relaxant activity with time. The sedative potential of isolated compounds 1 and 2 was determined by the open field model, both compounds showed good sedation ( p < 0.05 ) at 20 mg/kg. The anti-inflammatory potential of compound 1 was recorded by histamine-induced paw edema and carrageen paw edema model, and in both models, compounds 1 and 2 showed strong effect at 20 mg/kg. Binding orientations, binding energy values, and computed inhibition constants (Ki) values revealed that the studied compounds have a good to excellent inhibition potential against μ-opioid receptors and COX-2.

Analgesic, Anti-inflammatory, Antipyretic, and In Silico Measurements of Sonneratia caseolaris (L.) Fruits from Sundarbans, Bangladesh

“…Different mu (μ) receptors in our brain are responsible for central pain sensation. In a previous article, Aljohani et al conducted an in silico analysis of the central analgesic activity by taking the 5C1M protein of μ receptors [58].…”

“…Different mu ( μ ) receptors in our brain are responsible for central pain sensation. In a previous article, Aljohani et al conducted an in silico analysis of the central analgesic activity by taking the 5C1M protein of μ receptors [ 58 ]. We also took that 5C1M protein, and it was docked with the above-mentioned ligands and the standard drug tramadol.…”

Literature Review of the Pharmacological Activities of the Euphorbiaceae Family Plants

“…According to Singh et al, (2012), poinsettia extract can provide an anticonvulsant effect but does not affect the coordination of motor nerve cells and anxiety. The content of the flavonoids spinacetin and patuletin in poinsettia is thought to be able to have an excellent effect as an antiinflammatory, as evidenced by the decrease in oedema in rats' feet, besides that poinsettia also provides analgesic and sedative effects, and believed to have a muscle relaxant effect (Aljohani et al, 2022).…”

Enhancing Multiple Sequence Alignment with Genetic Algorithms: A Bioinformatics Approach in Biomedical Engineering

“…Due to the increasing scientific research in medicine-based informatics, the genetic algorithm has been tested using a set of genetic sequences from a group of vegetables of particular interest in medicine, such as Euphorbia pulcherrima, which has Anti-Inflammatory, Analgesic, Sedative, and Muscle-Relaxant Activities [29] . Preventing health complications is a significant motivation for studying the properties of vegetables.…”