In vivo anti-<b><i>Trichophyton</i></b> Activities of Seed Oil Obtained from <b><i>Caragana</i></b><b><i>korshinskii</i></b> Kom.

Abstract

Aim: The objective of this study was to evaluate the effects of seed oil of Caragana korshinskii Kom. against Trichophyton mentagrophytes on an in vivo guinea pig model of dermatophytosis. Methods: The skin of albino guinea pigs was infected with T. mentagrophytes, and the animals were divided into five groups: negative control (NC group), positive control (PC group), vehicle control, CK50% group (received topical 50% seed oil of C.korshinskii), and CK100% group (received topical 100% seed oil of C.korshinskii). Evaluation of clinical efficacy was performed 72 h after the completion of a 10-day treatment regimen. Skin biopsy samples were processed for histopathological examination. Results: The infected untreated control guinea pigs showed patches of hair loss and ulcerated or scaly skin. Lower clinical scores indicate improved efficacy compared with NC. The lesion scores significantly declined in the CK50%, CK100%, and PC groups in comparison with the NC group. The CK50% group (45.31%) and the CK100% group (75%) showed clinical efficacy compared with the PC group (78.13%). In addition, no fungal elements, inflammation, or tissue destruction was observed in any of the PAS-stained sections of the infected skin in the groups treated with CK100% or 1% terbinafine. Conclusion: Seed oil of C.korshinskii demonstrated high antifungal efficacy in experimental dermatophytosis.

Preparation, and Assessment of Antidermatophyte Activity of Miconazole–Urea Water-Soluble Film

“…Similar findings were reported by Garvey et al (2015) who stated that VT-1161 compound improved the onychomycosis clinically as well as mycologically, supporting the clinical development of VT-1161 as an antifungal agent. Zhou et al (2016) mentioned that analysis of skin sections of guinea pig infected untreated group revealed the presence of fungal elements, occasional inflammation, tissue destruction, and acanthosis that were clearly observed in PAS as well as H&E-stained sections. On the other hand, complete absence of fungal elements and tissue recovery were observed in the treatment group using CK100% formulation.…”

Antidermatophyte Activity of the Gentiopicroside-Rich n-Butanol Fraction from <b><i>Gentiana siphonantha</i></b> Maxim. Root on a Guinea Pig Model of Dermatophytosis

“…The animals were kept in rooms maintained at 24-28 °C, 70% humidity, 12 h/12 h light/dark cycle, and fed pelleted food and water ad libitum [38,39]. According to the procedure of our previous research, the guinea pig model of dermatophytosis was induced by the following method [40]. …”

“…The clinical evaluation consisted of a semi-quantitative score where the inoculated areas were evaluated and compared with negative-control animals according to the methodology described previously [38,39,40]. Briefly, the infected area on the back of each guinea pig was divided into 4 equal quadrants.…”