In vivo antimalarial activity of crude aqueous leaf extract of Pyrenacantha staudtii against Plasmodium berghei (NK65) in infected mice

Olorunniyi,, O. F; O., A. Morenikeji

doi:10.5897/ajpp2013.3950

Cited by 9 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could be supported by the observation that oleuropein and its metabolite (hydroxytyrosol), chief constituents of the leaf of O. europaea , are rapidly absorbed after oral administration with a maximum plasma concentration occurring 2 h after administration and rapidly distributed and excreted in urine [33, 34]. This is in agreement with other studies where crude extracts had less effect on established infection than early infection [35, 36].…”

Section: Discussionsupporting

confidence: 80%

Evaluation of the anti-malarial activity of crude extract and solvent fractions of the leaves of Olea europaea (Oleaceae) in mice

Misganaw

Engidawork

Nedi

2019

BMC Complement Altern Med

View full text Add to dashboard Cite

Background Drug resistance poses a challenge to malaria control measures. This calls for discovery & development of new chemotherapeutic agents. This study therefore was initiated to investigate the antimalarial activity of Olea europaea against P lasmodium berghei infected mice and to further ascertain in which fraction (s) the constituents responsible for anti-malarial activity are concentrated. Methods The leaves of Olea europaea were extracted by maceration using 80% methanol and the crude extract was then successively fractionated with solvents of differing polarity (chloroform, n-butanol and water). The anti-malarial activity of various doses of the extract and fractions (200, 400 and 600 mg/kg) was evaluated using chemo-suppressive, curative, and repository tests. Parameters, including parasitemia, rectal temperature, body weight, and packed cell volume were determined to establish the activity. Results The acute oral toxicity test result revealed that the LD50 values of the extract and fractions were greater than 2000 mg/kg in mice. The crude extract significantly reduced parasitemia ( p < 0.001) and prolonged survival time ( p < 0.001), in a dose-dependent manner, in all tests, as compared to the negative control group. Higher parasitemia suppression (58%) was achieved with the larger dose (600 mg/kg) in the 4-day suppressive test, suggesting that the crude extract has largely a chemo-suppressive activity. Parasitemia was significantly reduced ( p < 0.001) by all fractions in all doses used when compared to the negative controls, with the rank order of n-butanol (51%) > chloroform>aqueous (21%) fractions. Larger (600 mg/kg) and middle (400 mg/kg) doses of the crude extract as well as the fractions ameliorated all the other parameters in a consistent manner, with the crude being more active than the fractions. Preliminary phytochemical analysis revealed the presence of secondary metabolites that were differentially distributed in the fractions. Conclusion The findings collectively indicate that the plant is endowed with antimalarial activity, the activity being more in the crude extract than the fractions, owing to the presence of secondary metabolites that act independently or in synergy. The varying degree of antimalarial activity in the fractions suggests that non-polar and medium polar principles could be responsible for the observed activity.

show abstract

Section: Discussionsupporting

confidence: 80%

Evaluation of the anti-malarial activity of crude extract and solvent fractions of the leaves of Olea europaea (Oleaceae) in mice

Misganaw

Engidawork

Nedi

2019

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…Moreover, significant parasitemia suppression of the fraction was commenced after the initial dose, compared with the negative control (Figure 1). This indicates that the plant had profound rapid and sustained antimalarial effects in early and late infections in a dose-dependent manner, supported by similar studies 7,46 and supports the in vitro antiplasmodial activity of F. angolensis as well as its ethnobotanical uses. [12][13][14] On the other hand, prevention of body weight loss, amelioration of anemia, and stabilization of temperature in infected mice are important actions of a potent antimalarial drug.…”

supporting

confidence: 65%

Antimalarial Activity of Fagaropsis angolensis (Rutaceae) Crude Extracts and Solvent Fractions of Its Stem Bark Against Plasmodium berghei in Mice

Alemu

Misganaw

2021

JEP

View full text Add to dashboard Cite

“…Onyesom et al 17 and Dapper et al 28 demonstrated the antiplasmodial activity of P. amarus. Several other medicinal plants such as Pyrenacantha staudtii, 29 Aspilia africana, 30 Amaranthus spinosus L. and Andrographis paniculata Burm. f./Nees, 31 Piper betle 32 and Acacia auriculiformis 33 have also been shown to possess antimalarial activity.…”

Section: Resultsmentioning

confidence: 99%

Blood Schizonticidal Activity of Phyllanthus amarus Enhances Testovarian Antioxidant Defense Capacity in Plasmodium berghei Infected Mice

Ezedom¹,

Opajobi²,

Onyesom³

et al. 2018

TJNPR

View full text Add to dashboard Cite

Malaria is an infectious disease caused by a single-celled parasite belonging to the genus Plasmodium. There are more than 100 different species but Plasmodium falciparum is the deadliest among humans. 1 Malaria threatens the lives of more than one-third of the world's population and it remains a major killer of humans worldwide. The disease exists mainly in the tropical areas of Asia, Africa, and America, where it infects millions of people. Each year, 350 to 500 million cases of malaria occur worldwide 2 and more than 1 million of its victims, mostly young children and pregnant women, die annually. 2,3 It is known that malarial infection is accompanied by increased production of reactive oxygen species (ROS) and the malarial parasites are sensitive to oxidative damage. 3 The univalent reduction of oxygen results in a series of cytotoxic oxygen species such as superoxide anions (O2-) , hydrogen peroxide (H2O2) and hydroxyl radicals (OH-). 4 These highly ROS can cause a wide-spectrum of cell damage including lipid peroxidation, inactivation of enzymes, alteration of intracellular oxidation-reduction state and damage to DNA. 5-7

show abstract

In vivo antimalarial activity of crude aqueous leaf extract of Pyrenacantha staudtii against Plasmodium berghei (NK65) in infected mice

Cited by 9 publications

References 13 publications

Evaluation of the anti-malarial activity of crude extract and solvent fractions of the leaves of Olea europaea (Oleaceae) in mice

Evaluation of the anti-malarial activity of crude extract and solvent fractions of the leaves of Olea europaea (Oleaceae) in mice

Antimalarial Activity of Fagaropsis angolensis (Rutaceae) Crude Extracts and Solvent Fractions of Its Stem Bark Against Plasmodium berghei in Mice

Blood Schizonticidal Activity of Phyllanthus amarus Enhances Testovarian Antioxidant Defense Capacity in Plasmodium berghei Infected Mice

Contact Info

Product

Resources

About