In vivo assessment of new resorbable PEG–PPG–PEG copolymer/starch bone wax in bone healing and tissue reaction of bone defect in rabbit model

Suwanprateeb, Jintamai; Kiertkrittikhoon, Siripong; Kintarak, Jutatip; Suvannapruk, Waraporn; Thammarakcharoen, Faungchat; Rukskul, Pattaravit

doi:10.1007/s10856-014-5249-6

Cited by 16 publications

(12 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the 4th and 8th week, tibia specimens were examined by Micro-CT (SkyScan-1172 micro-CT scanner, Bruker, Switzerland). Oval areas 4-mm in diameter and 8-mm long were chosen as ROI ( Suwanprateeb et al, 2014 ; Dong et al, 2017 ). A reconstruction of the bitmap data set was obtained and used to build the 3D model.…”

Section: Methodsmentioning

confidence: 99%

Icariin Enhances Bone Repair in Rabbits with Bone Infection during Post-infection Treatment and Prevents Inhibition of Osteoblasts by Vancomycin

Zhang¹,

Shen

Mao³

et al. 2017

Front. Pharmacol.

View full text Add to dashboard Cite

Vancomycin is an effective antibiotic for treatment of bone infection caused by Staphylococcus aureus, however, a high local concentration of vancomycin might induce a delay in bone union. Icariin has been reported to suppress osteoclastogenes and promote osteogenesis. Our study aimed to investigate the effect of icariin on bone repair after anti-infection treatment in vivo and to explore the resisting effect of icariin on rat calvarial osteoblasts (ROBs) inhibited with high doses of vancomycin. Rabbits with bone infection of S. aureus were treated with implanted vancomycin-calcium sulfate (VCS) and icariin at 10.86 mg/kg/day for consecutive 8 weeks. Micro-CT, morphology, blood biochemistry were evaluated. In addition, ROBs were treated with vancomycin and icariin at different doses. Cell proliferation and differentiation capabilities, BMP2, Runx2, OPG, RANKL mRNA levels and protein expression were assessed. The results indicated that high dose of vancomycin significantly decreased bone mass and inhibited osteocalcin secretion; icariin increased these indicators compared with the single vancomycin treatment. Over 0.1 mg/mL of vancomycin inhibited the proliferation and differentiation of ROBs, while icariin resisted the inhibition of vancomycin by regulating cell cycle and promoting the Alkaline phosphatase (ALP) activity. Moreover, icariin promote bone formation by up-regulating BMP2/Runx2 and OPG/RANKL pathways. Icariin exhibited osteoplastic properties on osteoblasts that had been inhibited with high doses of vancomycin. Therefore, icariin is helpful for post-infection treatment of bone infection.

show abstract

Section: Methodsmentioning

confidence: 99%

Icariin Enhances Bone Repair in Rabbits with Bone Infection during Post-infection Treatment and Prevents Inhibition of Osteoblasts by Vancomycin

Zhang¹,

Shen

Mao³

et al. 2017

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…Bone wax is composed of beewax, paraffin, and isopropyl palmitate; refined beewax, which constitutes 88% of bone wax, is an insoluble material [5]. Because of the hydrophobic nature of bone wax [5], it is not absorbed into the application site and can cause numerous side-effects [6][7][8][9][10][11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Effects of poly (ethylene glycol-propylene glycol) copolymer on hemostasis and osteogenesis in a rat calvarial defect model

Kim

Yoon

Kim³

et al. 2020

Korean J Vet Res

View full text Add to dashboard Cite

This study aimed to evaluate the effects of a bioabsorbable bone hemostatic agent comprising poly (ethylene glycolpropylene glycol) copolymers (PEG-PPG) on hemostasis and osteogenesis. Bilateral 3 mm diameter calvarial defects were created in 99 male Sprague-Dawley rats. The defects were filled with PEG-PPG or bone wax. The defects of control group were left unfilled. Virtual autopsy was performed to evaluate bioabsorption. The calvaria were subjected to x-ray microtomography (microCT) and histological examination. Bone volume fraction (BV/TV) and bone mineral density (BMD) were measured using microCT; furthermore, white blood cell count and histological examination were performed. After application of PEG-PPG and bone wax, immediate hemostasis was achieved. Autopsy revealed that PEG-PPG disappeared within 48 h at the application site; in contrast, bone wax remained until 12 weeks. The PEG-PPG and control groups showed significantly more osteogenesis than the bone wax group with respect to BV/TV and BMD at 3, 6, and 12 weeks (p < 0.05). Histology revealed that the bone wax group exhibited little bone formation with inflammation. In contrast, PEG-PPG and control groups showed significantly more qualitative osteogenesis than the bone wax group (p < 0.01). In conclusion, PEG-PPG showed immediate hemostasis and was absorbed to allow progressive osteogenesis.

show abstract

“…The incorporation of pregelatinized starch provided better handling properties by increasing the stiffness, smoothness to the material and similar to bone wax it also prevents bleeding by acting as physical barrier. The in vivo analysis of the material in rabbit tibia defect models shows that it does not inhibit bone healing process and was resorbed from the sites within 2 days 72,73 . Another alternative to bone wax was PEG‐Calcium phosphate cement with pregelatinized starch which has the properties of hydrophilicity, malleability, and cohesiveness.…”

Section: Progress In Enhancing Sternal Healing Controlling Bleedingmentioning

confidence: 99%

Recent developments in controlling sternal wound infection after cardiac surgery and measures to enhance sternal healing

Pradeep

Rangasamy

Varma

2020

Medicinal Research Reviews

View full text Add to dashboard Cite

One of the major risks of cardiac surgery is the occurrence of infection at the sternal wound site. Sternal wound infections are primarily classified into superficial infection and deep sternal wound infection or mediastinitis. A patient is diagnosed with mediastinitis if microorganisms are present in their mediastinal tissue/fluid or with the observation of sternal wound infection during operation and with characteristic symptoms including chest pain, fever, and purulent drainage from the mediastinum. It is usually caused by Staphylococcal organisms in 75.8% of cases and the rest is caused by gram‐negative bacteria. Currently, in cardiac surgery, hemostasis is achieved using electrocautery and bone wax, and the sternum is closed using wire cerclage. Several studies show that bone wax can act as a nidus for initiation of infection and the oozing blood and hematoma at the site can promote the growth of infectious organisms. Many research groups have developed different types of biomaterials and reported on the prevention of infection and healing of the sternum. These materials are reported to have both positive and negative effects. In this review, we highlight the current clinical practices undertaken to prevent infection and bleeding as well as research progress in this field and their outcomes in controlling bleeding, infection, and enhancing sternal healing.

show abstract

In vivo assessment of new resorbable PEG–PPG–PEG copolymer/starch bone wax in bone healing and tissue reaction of bone defect in rabbit model

Cited by 16 publications

References 29 publications

Icariin Enhances Bone Repair in Rabbits with Bone Infection during Post-infection Treatment and Prevents Inhibition of Osteoblasts by Vancomycin

Icariin Enhances Bone Repair in Rabbits with Bone Infection during Post-infection Treatment and Prevents Inhibition of Osteoblasts by Vancomycin

Effects of poly (ethylene glycol-propylene glycol) copolymer on hemostasis and osteogenesis in a rat calvarial defect model

Recent developments in controlling sternal wound infection after cardiac surgery and measures to enhance sternal healing

Contact Info

Product

Resources

About