In vivo biocompatibility and vascularization of biodegradable porous polyurethane scaffolds for tissue engineering

Laschke, Matthias W.; Strohe, A.; Scheuer, Cláudia; Eglin, David; Verrier, Sophie; Alini, Mauro; Pohlemann, Tim; Menger, Michael D.

doi:10.1016/j.actbio.2009.02.006

Cited by 124 publications

(103 citation statements)

References 54 publications

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“…In the present study, to limit the degradation cascade, we sealed the end-plate tunnel using conductive scaffolds (Fig. 5d); [14,16,22]. The evaluation of the level of the tunnel's repair with an accurate histological analysis will be the goal of future studies.…”

Section: Discussionmentioning

confidence: 99%

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The transpedicular approach for the study of intervertebral disc regeneration strategies: in vivo characterization

et al. 2013

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Purpose To characterize in vivo the transpedicular approach (TA) as an alternative route to study intervertebral disc (IVD) regeneration strategies in a sheep model. Methods 48 IVD of 12 sheep were used. TA was performed under fluoroscopy, followed by nucleotomy (2-mm shaver resector). A polyurethane scaffold was used to repair the end-plate. X-ray and MRI images were acquired pre-, intra-and post-operatively (1, 3, 6 months). Complications were recorded. Results TA was feasible in all animals; nucleus pulposus (NP) from L1 to L5 was accessible. Nucleotomy followed by end-plate repair was achieved. Loss of NP signal intensity was shown in MRI images of the nucleotomy group. Conclusions TA is feasible in vivo, repeatable after only a short learning period and safely performed without significant morbidity. This animal model allows the study of IVD degeneration physiopathology and investigation of IVD regeneration techniques in vivo.

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Section: Discussionmentioning

confidence: 99%

“…3d. The elastomeric PU cylinder was synthesized as previously described [14][15][16]. The scaffold was sterilized by cold ethylene oxide process and stored at room temperature until the time of surgery.…”

Section: End-plate Repairmentioning

confidence: 99%

The transpedicular approach for the study of intervertebral disc regeneration strategies: in vivo characterization

et al. 2013

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“…Notably, it was found that the initial vascularisation of the scaffolds is crucially dependent on their biocompatibility. For instance, polyurethane scaffolds, which do not induce a leukocytic infl ammatory host tissue response, are only poorly vascularised throughout the fi rst 14 days after implantation (Laschke et al, 2009c(Laschke et al, , 2010a. In contrast, PLGA scaffolds, which induce a www.ecmjournal.org MW Laschke et al Biomaterials research with the skinfold chamber slight infl ammation, show a strong angiogenic reaction .…”

Section: Scaffolds For Tissue Engineeringmentioning

confidence: 99%

“…During the last few years, various scaffold types have been evaluated in terms of biocompatibility and vascularisation in dorsal skinfold chambers of mice. These scaffolds consisted of collagen (Ichioka et al, 2005), collagen-elastin (Ring et al, 2010a), collagen-chitosanhydroxyapatite hydrogel , acellular dentin (Rücker et al, 2008), hydroxyapatite (Rücker et al, 2008), β-tricalcium-phosphate , poly(ether ester) (Druecke et al, 2004), poly(L-lactideco-glycolide) (PLGA) , polyethylene glycol terephthalate/polybutylene terephthalate (PEGT/ PBT) (Ring et al, 2006a,b), lactocapromer terpolymer (Ring et al, 2010b(Ring et al, , 2011b, polyurethane (Laschke et al, 2009c(Laschke et al, , 2010a and supramolecular nanofi bres (Ghanaati et al, 2009). Notably, it was found that the initial vascularisation of the scaffolds is crucially dependent on their biocompatibility.…”

Section: Scaffolds For Tissue Engineeringmentioning

confidence: 99%

The dorsal skinfold chamber: window into the dynamic interaction of biomaterials with their surrounding host tissue

Laschke

Vollmar²,

Menger³

2011

eCM

146

128

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The implantation of biomaterials into the human body has become an indispensable part of almost all fi elds of modern medicine. Accordingly, there is an increasing need for appropriate approaches, which can be used to evaluate the suitability of different biomaterials for distinct clinical indications. The dorsal skinfold chamber is a sophisticated experimental model, which has been proven to be extremely valuable for the systematic in vivo analysis of the dynamic interaction of small biomaterial implants with the surrounding host tissue in rats, hamsters and mice. By means of intravital fl uorescence microscopy, this chronic model allows for repeated analyses of various cellular, molecular and microvascular mechanisms, which are involved in the early infl ammatory and angiogenic host tissue response to biomaterials during the initial 2-3 weeks after implantation. Therefore, the dorsal skinfold chamber has been broadly used during the last two decades to assess the in vivo performance of prosthetic vascular grafts, metallic implants, surgical meshes, bone substitutes, scaffolds for tissue engineering, as well as for locally or systemically applied drug delivery systems. These studies have contributed to identify basic material properties determining the biocompatibility of the implants and vascular ingrowth into their surface or internal structures. Thus, the dorsal skinfold chamber model does not only provide deep insights into the complex interactions of biomaterials with the surrounding soft tissues of the host but also represents an important tool for the future development of novel biomaterials aiming at an optimisation of their biofunctionality in clinical practice.

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“…They provide a porous interconnected environment for cells seeding and by-product circulation 25) . In addition, they have been demonstrated to be noncytotoxic in vitro and to exhibit a good in vivo biocompatibility without inducing a strong leukocytic inflammatory host tissue response after implantation 26) . In our previous study, we mixed SF and CS into a 3D scaffold provide unique chemical, structural, and mechanical properties, which could be utilized for bone tissue engineering and regeneration applications.…”

Section: Introductionmentioning

confidence: 99%

<i>In Vitro</i> Culture of BMSCs on VEGF-SF-CS Three-Dimensional Scaffolds for Bone Tissue Engineering

Tong

Xue

et al. 2015

J. Hard Tissue Biology.

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The objective of the study was to discuss the biocompatibility of VEGF-silk fibroin-chitosan (VEGF-SF-CS) three-dimensional scaffolds in order to offer an ideal scaffold for bone tissue engineering. Bone marrow derived mesenchymal stem cells (BMSCs) were seeded on the VEGF-SF-CS three-dimensional scaffolds and silk fibroin-chitosan (SF-CS) three-dimensional scaffolds. On VEGF-SF-CS and SF-CS three-dimensional scaffolds, the cell adhesion rate was increased as time went on. Scanning electron microscopy: The cells grew actively and had normal multiple fissions, granular and filamentous substrates could be seen around the cells, and cell microfilaments were closely connected with the scaffolds. The cells could not only show attached growth on surfaces of the scaffolds, but could also extend into the scaffolds. CCK-8 and ALP analysis proved that the vascular endothelial growth factor (VEGF) could significantly promote BMSCs growth and proliferation in the SF-CS scaffolds; however, the enhancement of BMSCs cell proliferation and activity by VEGF is dependent on time. Thus, VEGF-SF-CS composite scaffolds represent a promising new type of scaffold for bone tissue engineering.

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In vivo biocompatibility and vascularization of biodegradable porous polyurethane scaffolds for tissue engineering

Cited by 124 publications

References 54 publications

The transpedicular approach for the study of intervertebral disc regeneration strategies: in vivo characterization

The transpedicular approach for the study of intervertebral disc regeneration strategies: in vivo characterization

The dorsal skinfold chamber: window into the dynamic interaction of biomaterials with their surrounding host tissue

<i>In Vitro</i> Culture of BMSCs on VEGF-SF-CS Three-Dimensional Scaffolds for Bone Tissue Engineering

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