In vivo bioluminescence imaging for early detection and monitoring of disease progression in a murine model of neuroblastoma

Dickson, Paxton V.; Hamner, Blair; Ng, Catherine Y.; Hall, Marshall M.; Zhou, Junfang; Hargrove, Phillip W.; McCarville, M. Beth; Davidoff, Andrew M.

doi:10.1016/j.jpedsurg.2007.02.027

Cited by 57 publications

(51 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In cell tracking studies and for monitoring tumor burden, the intensity of the bioluminescent signal should primarily reflect the number of viable cells. Many validation studies in different tumor models have shown a good correlation between tumor cell number or volume and BLI signal intensity [4][5][6][7][8][9]. However this comparison/correlation should be assessed for every new reporter-transduced cell line and tumor model for possible confounding factors, such as the therapy itself or repeated injection of the substrate.…”

Section: Discussionmentioning

confidence: 99%

Plasma Protein Binding of Luciferase Substrates Influences Sensitivity and Accuracy of Bioluminescence Imaging

et al. 2010

View full text Add to dashboard Cite

Increasing concentrations of serum proteins showed an important negative effect on BLI signal intensity, both for D-luciferin and coelenterazine-dependent reactions. This was due to a decrease in the free fraction of the substrate in the presence of serum proteins of up to 88%. In vivo, hypoalbuminemia was associated with higher BLI signal intensity, although this was only significant in animals with a major decrease in albumin (<2 g/dL). Important kinetic differences, including earlier peak luminescence and a more rapid decline in luminescence, were observed for coelenterazine-dependent BLI under hypoalbuminemic conditions. Changes in protein concentrations can significantly influence BLI quantification and its presence decreases sensitivity in vitro. In vivo, effects on signal intensity and kinetics are only noted at severe hypoalbuminemia and can be neglected in most experimental designs. These findings again emphasize the need for careful interpretation of BLI quantification data.

show abstract

Section: Discussionmentioning

confidence: 99%

Plasma Protein Binding of Luciferase Substrates Influences Sensitivity and Accuracy of Bioluminescence Imaging

et al. 2010

View full text Add to dashboard Cite

show abstract

“…We have previously shown that the bioluminescent signal (photons per second) in untreated retroperitoneal neuroblastoma xenografts is directly proportional to the tumor burden (NB-1691 cell number) in vitro and in vivo (18). It was therefore surprising to observe that the effect of AAV IFN-h monotherapy on established retroperitoneal neuroblastomas, as assessed by bioluminescent imaging, seemed to be counter to the assessment by ultrasonography.…”

Section: Alterations Of the Intratumoral Vasculature With Aav Ifn-b Mmentioning

confidence: 99%

“…An orthotopic tumor model was included because previous studies have suggested that tumor location can have a significant effect on the angiogenic profile of a growing tumor (17). To study the effect of treatment noninvasively and in real time, these tumors were followed by both serial ultrasonography and bioluminescence imaging, the NB-1691 cells having been modified to stably express luciferase (18). Treatment of established retroperitoneal NB-1691 tumors was initiated 17 days following tumor cell inoculation, when tumors were a mean size of 0.13 F 0.03 cm 3 .…”

Section: Restricted Growth Of Established Tumors With Aav Ifn-b Monotmentioning

confidence: 99%

See 1 more Smart Citation

Continuous Delivery of IFN-β Promotes Sustained Maturation of Intratumoral Vasculature

Dickson¹,

Hamner²,

Streck³

et al. 2007

Molecular Cancer Research

Self Cite

View full text Add to dashboard Cite

IFNs have pleiotropic antitumor mechanisms of action. The purpose of this study was to further investigate the effects of IFN-B on the vasculature of human xenografts in immunodeficient mice. We found that continuous, systemic IFN-B delivery, established with liver-targeted adeno-associated virus vectors, led to sustained morphologic and functional changes of the tumor vasculature that were consistent with vessel maturation. These changes included increased smooth muscle cell coverage of tumor vessels, improved intratumoral blood flow, and decreased vessel permeability, tumor interstitial pressure, and intratumoral hypoxia. Although these changes in the tumor vasculature resulted in more efficient tumor perfusion, further tumor growth was restricted, as the mature vasculature seemed to be unable to expand to support further tumor growth. In addition, maturation of the intratumoral vasculature resulted in increased intratumoral penetration of systemically administered chemotherapy. Finally, molecular analysis revealed increased expression by treated tumors of angiopoietin-1, a cytokine known to promote vessel stabilization. Induction of angiopoietin-1 expression in response to IFN-B was broadly observed in different tumor lines but not in those with defects in IFN signaling. In addition, IFN-B -mediated vascular changes were prevented when angiopoietin signaling was blocked with a decoy receptor. Thus, we have identified an alternative approach for achieving sustained vascular remodeling-continuous delivery of IFN-B. In addition to restricting tumor growth by inhibiting further angiogenesis, maturation of the tumor vasculature also improved the efficiency of delivery of adjuvant therapy. These results have significant implications for the planning of combination anticancer therapy.

show abstract

“…This cell line was generated from a 10-year old female diagnosed with malignant glioma at St. Jude Children's Research Hospital (Memphis, TN). This tumor line has been modified to stably express firefly luciferase (Dickson et al, 2007). Luciferase-labeled SJG2 cell line was used to generate the cranial orthotopic tumor model.…”

Section: Tumor Linesmentioning

confidence: 99%

CNS Penetration of Tyrosine Kinase Inhibitors in Mouse Models

Elmeliegy¹

View full text Add to dashboard Cite

In vivo bioluminescence imaging for early detection and monitoring of disease progression in a murine model of neuroblastoma

Cited by 57 publications

References 20 publications

Plasma Protein Binding of Luciferase Substrates Influences Sensitivity and Accuracy of Bioluminescence Imaging

Plasma Protein Binding of Luciferase Substrates Influences Sensitivity and Accuracy of Bioluminescence Imaging

Continuous Delivery of IFN-β Promotes Sustained Maturation of Intratumoral Vasculature

CNS Penetration of Tyrosine Kinase Inhibitors in Mouse Models

Contact Info

Product

Resources

About