In vivo bone formation by human bone marrow cells: effect of osteogenic culture supplements and cell densities

Mendes, Sofia Silva; Brink, I. Van Den; Bruijn, Joost D. de; Blitterswijk, Clemens A. van

doi:10.1023/a:1008904413859

Cited by 43 publications

(33 citation statements)

References 20 publications

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“…During the culturing of cells on the scaffolds in order to produce constructs for the implantation, BGF and DEX were added into culturing medium, which is suggested to stimulate osteogenic differentiation [29,30]. After 7 days of culture, the extracellular matrix was formed in the scaffolds.…”

Section: Article In Pressmentioning

confidence: 99%

Biological performance in goats of a porous titanium alloy–biphasic calcium phosphate composite

Li¹,

Habibović²,

Yuan³

et al. 2007

Biomaterials

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Section: Article In Pressmentioning

confidence: 99%

Biological performance in goats of a porous titanium alloy–biphasic calcium phosphate composite

Li¹,

Habibović²,

Yuan³

et al. 2007

Biomaterials

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“…[13][14][15] The addition of proliferation (basic fibroblast growth factor, bFGF) and differentiation [recombinant human (rh)BMP-2] stimulating agents during culture has been shown to enhance the in vivo osteogenic potential. 16,17 The osteogenic potential of cellloaded scaffolds can further be increased by modifying the conditions during seeding, optimizing the number of loaded cells, [17][18][19][20] and culturing, for example, static versus dynamic culturing. [21][22][23] Examples of static methods are droplet and cell suspension seeding.…”

Section: Introductionmentioning

confidence: 99%

Bone formation in CaP‐coated and noncoated titanium fiber mesh

Vehof¹,

Dolder²,

Ruijter³

et al. 2003

J Biomedical Materials Res

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The osteogenic activity of calcium phosphate (CaP)-coated and noncoated porous titanium (Ti) fiber mesh loaded with cultured syngeneic osteogenic cells after prolonged in situ culturing was compared in a syngeneic rat ectopic assay model. Rat bone marrow (RBM) cells were loaded onto the CaP-coated and noncoated Ti scaffolds using either a droplet or a suspension loading method. After loading, the RBM cells were cultured for 8 days in vitro. Thereafter, implants were subcutaneously placed in 39 syngeneic rats. The rats were euthanized and the implants retrieved at 2, 4, and 8 weeks postoperatively. Further, in the 8 week group fluorochrome bone markers were injected at 2, 4, and 6 weeks. Histological analysis demonstrated that only the CaP-coated meshes supported bone formation. The amount of newly formed bone varied between single and multiple spheres to filling a significant part of the mesh porosity. In the newly formed bone, osteocytes embedded in a mineralized matrix could be observed clearly. On the other hand, in the noncoated titanium implants, abundant deposition of calcium-containing material was seen. This deposit lacked a bonelike tissue organization. Further analysis revealed that the cell-loading method did not influence the final amount of bone formation. In CaP-coated implants the accumulation sequence of the fluorochrome markers showed that bone formation started on the mesh fibers. In conclusion, our results prove that the combination of a thin CaP coating, Ti-mesh, and RBM cells can indeed generate ectopic bone formation after prolonged in vitro culturing. No effect of the loading method was observed on the final amount of bone.

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“…7 Evidently, for the final bone formation, the osteogenic potential of the cells as seeded in the scaffolds is very important. To enhance the osteogenic potential of the construct, several conditions can be modified during and after seeding by optimizing the number of loaded cells [8][9][10] and conditions during culturing. [11][12][13] Also, the addition of factors such as dexamethasone and BMP is known to direct the differentiation of mesenchymal stromal cells into the osteoblast lineage.…”

Section: Introductionmentioning

confidence: 99%

Bone formation by rat bone marrow cells cultured on titanium fiber mesh: Effect of in vitro culture time

Dolder

Vehof

Spauwen³

et al. 2002

J. Biomed. Mater. Res.

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The objective of this study was to examine the effect of cell culture time on bone formation by rat bone marrow cells seeded in titanium fiber mesh. As a seeding technique, a high cell suspension was used (3 x 10(6) cells/mL). Therefore, 30 meshes were repeatedly rotated in a 10 mL tube (containing 30 x 10(6) cells) on a rotation plate (2 rpm) for 3 h. Osteogenic cells were cultured for 1, 4, and 8 days on titanium fiber mesh and finally implanted subcutaneously in rats. Meshes without cells were also implanted subcutaneously in rats. DNA and scanning electron microscopy (SEM) analyses and calcium measurements determined cellular proliferation and differentiation during the in vitro incubation period of the mesh implants. Four weeks after implant insertion, the animals were sacrificed. The implants, with their surrounding tissue, were retrieved and prepared for histologic evaluation and calcium measurements. DNA analysis of the in vitro experiment showed a lag phase from day 1 through day 4, but a 42% increase in DNA between days 4 and 8. SEM and calcium measurements indicated an increase in calcium from day 1 to day 4, yet only a small but significant increase from days 4 to 8. Histologic analysis demonstrated that bone was formed in all day 1 and day 4 implants, and that the bone-like tissue was present uniformly through the meshes. The bony tissue was morphologically characterized by osteocytes embedded in a mineralized matrix, with a layer of osteoid and osteoblasts at the surface. The day 8 implants showed only calcium phosphate deposition in the titanium fiber mesh. Calcium measurements of the implants revealed that calcification in day 1 implants was significantly higher (p < 0.05) compared to day 4 and day 8 implants. No significant difference in calcium content existed between day 4 and day 8 implants. On the basis of our results, we conclude that 1) bone formation was generated more effectively in osteogenic cells by a short culture time after seeding in titanium fiber mesh; 2) dynamic cell seeding is probably more effective than static cell seeding; and 3) selection of the right cells from the heterogenous bone marrow population remains a problem.

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In vivo bone formation by human bone marrow cells: effect of osteogenic culture supplements and cell densities

Cited by 43 publications

References 20 publications

Biological performance in goats of a porous titanium alloy–biphasic calcium phosphate composite

Biological performance in goats of a porous titanium alloy–biphasic calcium phosphate composite

Bone formation in CaP‐coated and noncoated titanium fiber mesh

Bone formation by rat bone marrow cells cultured on titanium fiber mesh: Effect of in vitro culture time

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