In vivo cellular imaging of lymphocyte trafficking by MRI: A tumor model approach to cell‐based anticancer therapy

Смирнов, П. Р.; Lavergne, Elise; Gazeau, Florence; Lewin, M.; Boissonnas, Alexandre; Doan, Bich‐Thuy; Gillet, Brigitte; Combadière, Christophe; Combadière, Béhazine; Clémеnt, Olivier

doi:10.1002/mrm.20996

Cited by 92 publications

(99 citation statements)

References 35 publications

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“…Moreover, to make the study more relevant, concentrations were selected that resembled doses at which these NPs are used for human applications. An IC 20 (concentrations that lead to 20% loss in viability) value for 24 h incubation was considered, as in all three cases, MCM-41 (100 µg/mL) 27,28 Fe 2 O 3 (100 µg/mL) 29,30 and ZnO (12.5 µg/ mL) 31,32 the concentrations chosen were within the range used for biomedical or other applications.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Toxicity and Gene Expression Changes Triggered by Oxide Nanoparticles

Dua¹,

Chaudhari²,

Lee³

et al. 2011

Bulletin of the Korean Chemical Society

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Several studies have demonstrated that nanoparticles (NPs) have toxic effects on cultured cell lines, yet there are no clear data describing the overall molecular changes induced by NPs currently in use for human applications. In this study, the in vitro cytotoxicity of three oxide NPs of around 100 nm size, namely, mesoporous silica (MCM-41), iron oxide (Fe 2 O 3 -NPs), and zinc oxide (ZnO-NPs), was evaluated in the human embryonic kidney cell line HEK293. Cell viability assays demonstrated that 100 µg/mL MCM-41, 100 µg/mL Fe 2 O 3 , and 12.5 µg/mL ZnO exhibited 20% reductions in HEK293 cell viability in 24 hrs. DNA microarray analysis was performed on cells treated with these oxide NPs and further validated by real time PCR to understand cytotoxic changes occurring at the molecular level. Microarray analysis of NP-treated cells identified a number of up-and down-regulated genes that were found to be associated with inflammation, stress, and the cell death and defense response. At both the cellular and molecular levels, the toxicity was observed in the following order: ZnO-NPs > Fe 2 O 3 -NPs > MCM-41. In conclusion, our study provides important information regarding the toxicity of these three commonly used oxide NPs, which should be useful in future biomedical applications of these nanoparticles.Key Words : MCM-41, Fe 2 O 3 nanoparticle, ZnO nanoparticle, HEK293, Microarray Introduction Nanoparticles (NPs) have increased surface area to weight ratios relative to the same materials in the non-nano size range. In addition to unusual physical properties, NPs are also more chemically reactive than larger particles, which can be either advantageous or harmful depending on their end use. The nanotech boom started around a decade ago and since then the use of NPs in consumer goods and biomedical applications has dramatically increased. NPs are increasingly applied as drug delivery vehicles, biosensors, imaging contrast reagents, and therapeutic agents. However, it is surprising that even today the scientific and industrial community has no sufficiently clear data on the overall effects of these NPs on human health.NPs are easily internalized into cells 1,2 and some NPs have even been shown to cross the blood brain barrier 3-5 where they alter biological processes and cause toxicity. Various in vitro and in vivo studies indicate that some NPs are associated with serious toxicity issues. The most commonly used in vitro toxicity tests focus on whether potentially toxic agents result in cell death. However, sublethal cellular changes may also exist that are not visible in such toxicity screens, but may significantly affect biological processes at the organismal level. Hence, it is important to identify overall cellular changes mediated by NP exposure. Identification of molecular signatures of toxicity at the genomic or proteomic levels provides comprehensive and reliable high throughput data. These data can then be used to compare, classify, and grade a NP on a scale of toxicity. In the present study we identified the m...

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Section: Resultsmentioning

confidence: 99%

Evaluation of Toxicity and Gene Expression Changes Triggered by Oxide Nanoparticles

Dua¹,

Chaudhari²,

Lee³

et al. 2011

Bulletin of the Korean Chemical Society

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“…Because nonspecifi c internalization by T cells is ineffi cient, contrast labeling of those cells requires tagging with internalization enhancers such as peptides or specifi c ligands that facilitate the internalization of the contrast agent without compromising cellular function. A variety of these internalization enhancers have been proposed and used effectively, including HIV tat peptides (which can increase T-cell uptake of liposomal 33 and iron oxide nanoparticles 34 100-fold compared with unlabeled contrast agent), anionic iron oxide nanoparticles, 35 and transfection reagents combined with the contrast agent. 36 But while supermagnetic iron oxide (SPIO) and gadoliniumbased agents are well-established, safe, and effective MR contrast agents, little has been achieved from the standpoint of incorporating these contrast agents into drug delivery vehicles for targeting specifi c T cells.…”

Section: Tracking T-cell Trafficking and Migrationmentioning

confidence: 99%

Nanosystems for simultaneous imaging and drug delivery to T cells

Fahmy

Fong

Park

et al. 2007

AAPS J

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“…However, we found the content of accumulated Fe in the grafts to be a little bit lower than expected. Smirnov et al reported 37 that PB staining stained only a fraction (Fe 3+ ) of the studied particles (Fe 3+ and Fe 2+ ) and that some labeled cells were probably undetected, but detectable by MRI. We think this might be the explanation of our results.…”

Section: Discussionmentioning

confidence: 96%

Bcl-2-functionalized ultrasmall superparamagnetic iron oxide nanoparticles coated with amphiphilic polymer enhance the labeling efficiency of islets for detection by magnetic resonance imaging

Yang¹,

Cai²,

Qin³

et al. 2013

IJN

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In vivo cellular imaging of lymphocyte trafficking by MRI: A tumor model approach to cell‐based anticancer therapy

Cited by 92 publications

References 35 publications

Evaluation of Toxicity and Gene Expression Changes Triggered by Oxide Nanoparticles

Evaluation of Toxicity and Gene Expression Changes Triggered by Oxide Nanoparticles

Nanosystems for simultaneous imaging and drug delivery to T cells

Bcl-2-functionalized ultrasmall superparamagnetic iron oxide nanoparticles coated with amphiphilic polymer enhance the labeling efficiency of islets for detection by magnetic resonance imaging

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