In vivo Characteristics of a Transplantable Marek's Disease Lymphoblastoid Cell Line, MDCC-MSB1-41C

Matsuda, Haruo; Okamoto, Junya; Sekiya, Yuji; Yamada, Masao; Uno, Fumio; Murata, Masayoshi; Nii, Shiro

doi:10.2307/1590200

Cited by 4 publications

(4 citation statements)

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“…However, very little is k n o w n about the replication of M D V group in M D t u m o r cell lines. The present study was conducted to characterize the D N A structure of herpes-type viruses isolated from two independent cultures of MSB1-41C cells, a non-virus producing subline of M D C C -M S B 1 [18].…”

Section: Introductionmentioning

confidence: 99%

Replicating Marek's disease virus (MDV) serotype 2 DNA with inserted MDV serotype 1 DNA sequences in a Marek's disease lymphoblastoid cell line MSB1-41C

Hirai

Yamada

Arao

et al. 1990

Archives of Virology

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We characterized the properties of herpes-type viruses which grew well in a Marek's disease lymphoblastoid cell line, MSB1-41C, inducing cytopathic effect characterized by the formation of syncytial giant cells. Examination of the infectious virus by field inversion gel electrophoresis revealed the presence of DNA of about 180 kbp in both the culture fluid and cell fractions of the infected MSB-41C cells. The DNA was found to consist of Marek's disease virus (MDV) serotype 2 (MDV2) and MDV serotype 1 (MDV1) DNA by Southern blot hybridization. The MDV1 DNA consisted of sequences mainly from the long inverted repeats including multiple copies of 132 bp direct tandem repeats. Molecular cloning of BamHI digests of the MDV2 DNA revealed a fragment of MDV1 DNA and MDV2 DNA fused together, indicating that the recombinant MDV2 DNA had been generated by genetic recombination with the latent MDV1 DNA.

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Section: Introductionmentioning

confidence: 99%

Replicating Marek's disease virus (MDV) serotype 2 DNA with inserted MDV serotype 1 DNA sequences in a Marek's disease lymphoblastoid cell line MSB1-41C

Hirai

Yamada

Arao

et al. 1990

Archives of Virology

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“…Important differences between these two tumor cell lines are the capacity to develop tumors and to adhere to the culture dish [16]. In this report, we have contrasted the biochemical properties of adherent MSB1-41C and non adherent MSB1 cell lines.…”

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confidence: 97%

“…A Marek's disease (MD) lymphoblastoid T cell line, MDCC-MSB1-41C (MSB1-41C), was highly transplantable and metastatic in vivo, compared with the parental cell line MDCC-MSB1 (MSB1) [16]. Important differences between these two tumor cell lines are the capacity to develop tumors and to adhere to the culture dish [16].…”

mentioning

confidence: 99%

“…Four chicken lymphoblastoid cell line, adherent MSB1-41C [16,21] and 1104 X5 [12] lines and non-adherent MSB1 [1] and RP9 [17] lines, were cultured in RPMI 1640 medium (RPMI), supplemented with 10% heat-inactivated fetal bovine serum, glutamine (2 mM), penicillin (100 U/ml) and streptomycin (100 µg/ml). CEF cells free from endogenous virus were prepared from fertile eggs obtained from Nippon Institute of Biological Science, Yamanashi, Japan.…”

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Analysis of Extracellular Matrix Proteins in Malignant Chicken Cell Lines

Koyama

Nakajima

Miura

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. The adherent chicken cell line, MDCC-MSB1-41C, was highly transplantable and metastatic in vivo, compared with the parental non-adherent cell line MDCC-MSB1 from Marek's disease (MD) lymphoblastoid tumor. For clarification of differences in extracellular matrix proteins in MSB1-41C and MSB1 cells, examination was made of various components of extracellular matrix proteins. A detachment experiment indicated the protein(s) recognizing the Arg-Gly-Asp (RGD) sequence, the minimum structure required for recognition by the cell-surface receptors, is essential for the adherent character. Immunoblot assay using antibodies showed increased expression of fibronectin, fibronectin receptors, and vinculin on MSB1-41C cell lines. RGD-directed integrins mediate important cell-cell adhesive interaction and these interactions with extracellular matrix proteins may thus possibly be requisite for migration, proliferation and metastatic dissemination of MDCC-MSB1-41C cells. The RGD-containing peptide in the culture medium could cause detachment of cultured adhesive lymphoid leukosis LSCC-1104X5 cells from the dish too. -KEY WORDS: chicken cell line, fibronectin, vinculin.

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Genetic Resistance to a Marek's Disease Transplantable Tumor Cell Line in Chicken Lines Selected for Different Immunological Characters

et al. 1991

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The tumor incidence and mortality of Marek's disease (MD) were determined for 471 progeny from four pairs of lines two-way selected for different immunological characters: graft versus host reaction (GVHR) competence, IgG levels, antibody response to rabbit serum albumin (RSA), and anaphylactic shock to BSA. All chicks were inoculated at 1 wk of age with 1 to 2 x 10(6) viable cells of a MD lymphoblastoid cell line, MDCC-MSB1-41C (41 C). Tumor incidence differed significantly between the high (H) and low (L) lines of each GVHR-, IgG-, RSA-, or BSA-selected group. There were also significant differences in mortality rates between H and L lines of all selected groups. The IgG-H, BSA-H, and RSA-H lines were more resistant than their respective L lines. The H line of the GVHR-selected lines was more susceptible than the L line. Tumor regression could be detected only in the IgG- and RSA-selected lines. No definite correlation could be found between genetic resistance to 41C and genetic resistance to MD virus.

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In vivo Characteristics of a Transplantable Marek's Disease Lymphoblastoid Cell Line, MDCC-MSB1-41C

Cited by 4 publications

References 10 publications

Replicating Marek's disease virus (MDV) serotype 2 DNA with inserted MDV serotype 1 DNA sequences in a Marek's disease lymphoblastoid cell line MSB1-41C

Replicating Marek's disease virus (MDV) serotype 2 DNA with inserted MDV serotype 1 DNA sequences in a Marek's disease lymphoblastoid cell line MSB1-41C

Analysis of Extracellular Matrix Proteins in Malignant Chicken Cell Lines

Genetic Resistance to a Marek's Disease Transplantable Tumor Cell Line in Chicken Lines Selected for Different Immunological Characters

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