ObjectiveThis study evaluated experimentally-induced periapical bone loss sites using
digital radiographic and histopathologic parameters. Material and MethodsTwenty-seven Wistar rats were submitted to coronal opening of their mandibular
right first molars. They were radiographed at 2, 15 and 30 days after the
operative procedure by two digital radiographic storage phosphor plates
(Digora®). The images were analyzed by creating a region of interest
at the periapical region of each tooth (ImageJ) and registering the corresponding
pixel values. After the sacrifice, the specimens were submitted to microscopic
analysis in order to confirm the pulpal and periapical status of the tooth. ResultsThere was significant statistically difference between the control and test sides
in all the experimental periods regarding the pixel values (two-way ANOVA;
p<0.05). ConclusionsThe microscopic analysis proved that a periapical disease development occurred
during the experimental periods with an evolution from pulpal necrosis to
periapical bone resorption.