In vivo decreased expression of CD25 (p55 chain of IL-2 receptor) on CD3+ T cells correlates with low in vitro responsiveness to Plasmodium falciparum antigen in subjects living in a malaria endemic area

Chizzolini, Carlo; Nicholson, Janet K. A.; Geinoz, Anne; Olsen-Rasmussen, Melissa; Schrijvers, Dirk

doi:10.1016/0090-1229(91)90064-h

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…In addition, we do not yet know the frequency of antigen-reacting T cells. A lower proliferative response in malaria-infected individuals to purified antigen or peptides than to whole-parasite lysate was reported (5).…”

Section: Discussionmentioning

confidence: 97%

T-cell recognition of a cross-reactive antigen(s) in erythrocyte stages of Plasmodium falciparum and Plasmodium yoelii: inhibition of parasitemia by this antigen(s)

et al. 1993

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In the current study, we investigated the presence of a cross-reactive antigen(s) in the erythrocyte stage from Plasmodium yoelii (265 BY strain) and Plasmodium falciparum through recognition by T cells primed in vivo with antigens from each of these parasites. BALB/c mice are naturally resistant to P. falciparum but are susceptible to P. yoelii infection. Mice that had recovered from P. yoelii primary infection became resistant to a second infection. A higher in vitro proliferative response to a soluble blood stage preparation ofP. falciparum was observed in splenic cells from immune animals than in those from mice with a patent P. yoelii infection.The antigen-induced proliferative response was enhanced when animals were exposed to a secondary infection. Animals exposed to a challenge infection were treated with anti-CD4 or anti-CD8 monoclonal antibodies to deplete the corresponding subset of T cells. There was a marked diminution in P. falciparum antigen-induced proliferative response in the total splenic cell populations from CD8-depleted but not from CD4-depleted mice. In CD8-depleted and nondepleted animals, the antigen-induced proliferation in the total cell populations was markedly lower than in the T-cell-rich populations, indicating inhibitory activities of B cells and/or macrophages. There was no such difference in the stimulation between total and T-enriched cell populations from CD4-depleted animals. Flow cytometry analysis demonstrated the presence of an almost equal percentage of CD8+ (59.6%) and CD4+ (64%) T cells in the spleen preparations following in vivo depletion of CD4and CD8-bearing T cells, respectively. When cultured with P. yoelii blood stage antigen, splenocytes from animals immunized with P. fakiparum antigen displayed a significant proliferative response which was markedly diminished by treatment with anti-Thy-1.2 antibody plus complement. Animals immunized with P. falciparum antigen and then challenged with P. yoelii blood stage parasites displayed about a 50%o lower level of parasitemia. These results demonstrated the existence of a cross-reactive antigen(s) between a murine and a human Plasmodium species, as determined from both in vivo and in vitro biological assays, and indicated the

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Section: Discussionmentioning

confidence: 97%

T-cell recognition of a cross-reactive antigen(s) in erythrocyte stages of Plasmodium falciparum and Plasmodium yoelii: inhibition of parasitemia by this antigen(s)

et al. 1993

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Immune Escape Mechanisms are Plasmodium ’ s Secret Weapons Foiling the Success of Potent and Persistently Efficacious Malaria Vaccines

Farooq

Bergmann‐Leitner

2015

Clinical Immunology

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Immunological markers of childhood fevers in an area of intense and perennial malaria transmission

Hurt

Thein

Smith

et al. 1995

Clinical and Experimental Immunology

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SUMMARYIn order to describe presumed paediatric malaria on a cell-immunological basis, the soluble receptors of IL-2 {sIL-2R) and tumour necrosis factor (sTNF-R55 and sTNF-R75) were quantified in highly exposed young Tanzanian children. Sera were obtained from 66 acute and 72 reported febrile patients during health post consultations and follow-ups and from 68 community controls. Levels of sIL-2R, sTNF-R55 and sTNF-R75 were significantly elevated during fever attacks, especially in very young children. Soluble TNF-R75 levels were most stable and those of sTNF-R55 least. Levels of sTNF-R55 were related to the magnitude of fever and thus appeared to reflect attack severity. Levels of sTNF-R75 were highly significantly associated with parasite density, indicating that this response is malaria-specific. The present study indicates that sTNF-R75 levels could become a useful immunological tool in malaria intervention studies, as they reflect changes in ma I aria-specific immune responses. Future studies should validate this potential in different endemic settings.

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In vivo decreased expression of CD25 (p55 chain of IL-2 receptor) on CD3+ T cells correlates with low in vitro responsiveness to Plasmodium falciparum antigen in subjects living in a malaria endemic area

Cited by 3 publications

References 19 publications

T-cell recognition of a cross-reactive antigen(s) in erythrocyte stages of Plasmodium falciparum and Plasmodium yoelii: inhibition of parasitemia by this antigen(s)

T-cell recognition of a cross-reactive antigen(s) in erythrocyte stages of Plasmodium falciparum and Plasmodium yoelii: inhibition of parasitemia by this antigen(s)

Immune Escape Mechanisms are Plasmodium ’ s Secret Weapons Foiling the Success of Potent and Persistently Efficacious Malaria Vaccines

Immunological markers of childhood fevers in an area of intense and perennial malaria transmission

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