In vivo detection of chemiluminescence to monitor photodynamic threshold dose for tumor treatment

Abstract

During photodynamic therapy (PDT) it is important to monitor the treatment progress to achieve the intended therapeutic outcome. Chemiluminescence (CL) provides a sensitive and selective means for (1)O(2) detection. In our study, by comparing CL with the corresponding in situ PDT treatment protocols and the treatment effect, the results indicate that the treatment outcome for tumors was governed by a set of dosimetry factors with a rather complicated relationship, but there was a remarkable connection between tumor treatment effect and CL. In conclusion, CL can be used in in vivo PDT to determine the dose of tumor treatment and monitor the treatment threshold. By monitoring CL it is feasible to determine PDT dose to predict tumor treatment effect.

Effects of pulse width and repetition rate of pulsed laser on kinetics and production of singlet oxygen luminescence

“…detection. 18 The results indicate that a good correlation between the cumulative chemiluminescence and the PDT treatment outcome. 18 In addition, less 1 O 2 was produced at a higher light°uence rate.…”

“…18 The results indicate that a good correlation between the cumulative chemiluminescence and the PDT treatment outcome. 18 In addition, less 1 O 2 was produced at a higher light°uence rate. 18 One possible reason is rapid depletion of oxygen due to PDT photochemical reactions that induced a local hypoxia.…”

“…18 In addition, less 1 O 2 was produced at a higher light°uence rate. 18 One possible reason is rapid depletion of oxygen due to PDT photochemical reactions that induced a local hypoxia. In our study, 1 O 2 was directly measured by its luminescence at 1270 nm generated from photosensitization of RB solution in cuvette, which was exposed to room air at the top.…”

Ultrasound-Enhanced Chemiluminescence for Bioimaging

“…For example, the generation of firefly luciferin involves a chemical reaction involving luciferase, magnesium Dodeigne et al, 2000;Bedouhène et al, 2017;Jancinová et al, 2017;Liu et al, 2019 Coelenterazine Dubuisson et al, 2005;Mu, 2005;Diaz et al, 2018;Nishihara et al, 2018;Nomura et al, 2019 Peroxyoxalic acid and its derivatives CL is produced through energy transfer to a fluorescer from a fluorescent sensitizer, which is excited in a S1 state. Wang J. et al, 2002;Qin et al, 2005;Wei et al, 2011Wei et al, , 2016 and adenosine triphosphate (ATP), to yield luminescent luciferin and by-products like adenosine monophosphate, CO 2 , and pyrophosphate (Marques and Esteves da Silva, 2009;Kim et al, 2015). The mechanism of this reaction is addressed in Figure 1.…”

“…Researchers have developed a novel method to diagnose superficial tumors by photodynamic diagnosis mediated by CL probe containing FCLA (Wang J. et al, 2002). Wei et al (2011) utilized FCLA CL to monitor tumor necrosis in response to photodynamic therapy. First the FCLA was injected subcutaneously in mice, and light irradiation was provided after a 1 h. A near linear relationship was observed between the extent of damage from PDT, and the CL (Wei et al, 2011).…”