2020
DOI: 10.1038/s42003-020-1018-x
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In vivo dual RNA-seq reveals that neutrophil recruitment underlies differential tissue tropism of Streptococcus pneumoniae

Abstract: Streptococcus pneumoniae is a genetically diverse human-adapted pathogen commonly carried asymptomatically in the nasopharynx. We have recently shown that a single nucleotide polymorphism (SNP) in the raffinose pathway regulatory gene rafR accounts for a difference in the capacity of clonally-related strains to cause localised versus systemic infection. Using dual RNA-seq, we show that this SNP affects expression of bacterial genes encoding multiple sugar transporters, and fine-tunes carbohydrate metabolism, a… Show more

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Cited by 24 publications
(18 citation statements)
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“…Other RNA-sequencing (RNA-seq) studies have also focused on the host response within the lungs upon pneumococcal infection. These have identified key genes involved in neutrophil recruitment and response to diverse strains and mutants ( 12 , 13 ). Other forms of sequencing-based studies, such as transposon sequencing (Tn-seq) have also been performed on the pneumococcus in multiple contexts, such as in vivo survival or transmission, in multiple hosts ( 14 16 ).…”
mentioning
confidence: 99%
“…Other RNA-sequencing (RNA-seq) studies have also focused on the host response within the lungs upon pneumococcal infection. These have identified key genes involved in neutrophil recruitment and response to diverse strains and mutants ( 12 , 13 ). Other forms of sequencing-based studies, such as transposon sequencing (Tn-seq) have also been performed on the pneumococcus in multiple contexts, such as in vivo survival or transmission, in multiple hosts ( 14 16 ).…”
mentioning
confidence: 99%
“…Although this recruitment of LysM + appears to be too slow to account for the rapid fall in CFUs over the first 30 min in the dorsal skull preparation, after the subsequent rebound, the number of CFUs begins to fall again at less than 5h post-infection, as the LysM + population approaches its maximum. It appears therefore, that the LysM + population increases until the clearance of Sp is established 68,93 , then begins to fall. Intense immune reactions in the pachymeninx (rather than the leptomeninx) have been much studied for their occurrence in migraine 21 , and also observed in experimental autoimmune encephalitis 94,95 , trypanosomiasis 41 and infection by lymphocytic choriomeningitis virus 72 .…”
Section: Discussionmentioning
confidence: 98%
“…However, we do not know if the reduced efficacy of C27 in this model reflects an inability to prevent dissemination, and/or distinct features of the ST3 clinical strain, B2. Tissue specific differences in virulence have been identified for other STs (36,37), but further work is needed to dissect the roles that humAbs and ST3 strain specific differences may play in the reduced (which was not certified by peer review) is the author/funder. All rights reserved.…”
Section: Discussionmentioning
confidence: 99%