In vivo dynamic light scattering measurements of red blood cell aggregation

Fine, Ilya; Kaminsky, Alexander

doi:10.1117/12.699503

Cited by 5 publications

(3 citation statements)

References 3 publications

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“…From (8) and (9) Γ reaches the maximum for π θ = , which is the case of backscattering geometry. Apparently, the reflection measurement geometry appears to be associated with backscattering signal.…”

Section: Single Scattering By the Rbc'smentioning

confidence: 95%

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Speckle-based measurement of the light scattering by red blood cells in vivo

Fine

Kaminsky

2011

SPIE Proceedings

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Optical spectroscopy approach, using non-coherent light sources, has become an important tool for non-invasive analysis in vivo. It is based on the assumption that biochemical characteristics of biological system can be determined through the optical coefficients of blood and tissue particles. Thus, in the framework of this approach, the major concern is to express the obtained optical signals in terms the optical coefficients of the single particle of blood or tissue. However, since the light propagation in tissue is dominated by the multiple-scattering component, a direct measurement of single scattering characteristics turns to be a very difficult task. Practically, only the relative changes of absorption and scattering coefficients are measured. We suggested to adopt the dynamic light scattering (DLS) or speckle technique for the determination of the light scattering coefficients of the red blood cells under stasis conditions in vivo. We assumed that under zero flow conditions the RBC movement is driven mostly by the Brownian motion. It was shown, that under appropriate measurement geometry, the measured optical signal can be decomposed into a few major components. The most dominant components are ascribed to the single backscattering and forward scattering coefficients of the red blood cells. In-vitro and in vivo experimental tests have shown a good correspondence between the theoretically estimated and experientially measured results. The obtained results indicate that the DLS technique can be adopted for the determination of blood particles scattering characteristics in addition to the movement and effective viscosity parameters measurement in vivo.

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Section: Single Scattering By the Rbc'smentioning

confidence: 95%

“…If the diffusion light approximation is an applicable solution for semi-infinite media , the general expression (17) can be reduced into the measurable parameter 9 .…”

Section: Multiple Scatteringmentioning

confidence: 99%

Speckle-based measurement of the light scattering by red blood cells in vivo

Fine

Kaminsky

2011

SPIE Proceedings

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“…The principle behind dynamic light scattering is based on the Brownian motion of particles, which results in the fluctuation of scattered light intensity as the particles move. We have previously described for the first time the dynamics of the behavior of the DLS signal on a finger where blood flow was stopped [18],. We suggested that in the absence of blood flow, red blood cells gradually begin to behave like Brownian particles and the DLS signal reflects the dynamics of such particles.…”

Section: Introductionmentioning

confidence: 99%

A new non-invasive method for assessment of inflammation

Fine¹,

Kaminsky²,

Shenkman³

et al. 2023

Preprint

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This article examines the potential for non-invasive measurement of elevated levels of erythrocyte aggregation in vivo, which are linked to a higher risk of inflammatory processes. The study proposes the use of a dynamic light scattering approach to measure aggregability. The mDLS sensor modules consist of VICEL and two photodiodes, and two of these modules are placed on an inflatable transparent cuff. The cuff is then fitted to the subject's finger root, with one sensor module placed on each side of the root. Blood flow is temporarily halted for one minute using over-systolic inflation of the cuff, and signals from both sensors are recorded. Three groups of subjects were studied: a control group of 65 individuals, a group of 29 hospitalized COVID-19 patients, and a group of 34 hospitalized patients with inflammatory diseases. The experimental results demonstrated significant differences in signal kinetic behavior between the control group and the other two groups. These differences were attributed to the rate of RBC aggregation, which is associated with inflammation. The study underscores the potential for non-invasive diagnostic tools to assess inflammatory processes by analyzing RBC aggregation.

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A non-invasive method for the assessment of hemostasis in vivo by using dynamic light scattering

et al. 2011

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We present a new non invasive method for assessing hemostasis in vivo. This method is based on the analysis of the movement characteristics of red blood cells (RBCs) during blood stasis condition. Stasis is intermittently induced by occlusion of arterial blood flow at the finger root. We assumed that under zero flow conditions, RBC movement is driven mostly by Brownian motion, and we characterized the RBC movement by utilizing the dynamic light scattering (DLS) technique in vivo. We found that during the stasis the RBCs diffusion coefficient in plasma decreases. We speculate that the RBC diffusion coefficient is most strongly related to endothelial and hemostatic activity. This assumption is supported by our findings that RBC move ment, being expressed through the characteristics of the measured DLS signal, is correlative to the biological age and also is related to the coagulation factors. This new method can serve as a new diagnostic and research tool for the assessment of hemostasis and vascular function.

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In vivo dynamic light scattering measurements of red blood cell aggregation

Cited by 5 publications

References 3 publications

Speckle-based measurement of the light scattering by red blood cells in vivo

Speckle-based measurement of the light scattering by red blood cells in vivo

A new non-invasive method for assessment of inflammation

A non-invasive method for the assessment of hemostasis in vivo by using dynamic light scattering

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